Strain Fitness in Escherichia coli BW25113 around b3621

Experiment: Cholinium hydrogen phosphate 10 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Cholinium hydrogen phosphate 10 vol%
3,793,011	+				+1.2
3,793,021	+				+0.2
3,793,021	+				+0.1
3,793,021	+				+0.4
3,793,034	+				+1.2
3,793,124	-	rfaF	b3620	0.16	-1.5
3,793,169	+	rfaF	b3620	0.21	+0.4
3,793,234	-	rfaF	b3620	0.27	+1.0
3,793,259	-	rfaF	b3620	0.29	+0.5
3,793,305	-	rfaF	b3620	0.34	+0.3
3,793,307	+	rfaF	b3620	0.34	+1.8
3,793,307	+	rfaF	b3620	0.34	+3.0
3,793,307	+	rfaF	b3620	0.34	-0.2
3,793,307	-	rfaF	b3620	0.34	-1.2
3,793,311	+	rfaF	b3620	0.34	+0.5
3,793,328	-	rfaF	b3620	0.36	-0.0
3,793,350	+				-0.8
3,793,353	+	rfaF	b3620	0.38	+0.7
3,793,358	-	rfaF	b3620	0.39	+1.3
3,793,358	-	rfaF	b3620	0.39	+0.4
3,793,393	+	rfaF	b3620	0.42	-0.9
3,793,393	+	rfaF	b3620	0.42	-0.1
3,793,393	+	rfaF	b3620	0.42	+0.3
3,793,513	+	rfaF	b3620	0.54	+0.9
3,793,543	+	rfaF	b3620	0.56	+1.5
3,793,546	+	rfaF	b3620	0.57	+1.2
3,793,554	-	rfaF	b3620	0.57	-0.8
3,793,554	-	rfaF	b3620	0.57	+1.2
3,793,589	+	rfaF	b3620	0.61	+1.9
3,793,589	+	rfaF	b3620	0.61	+0.3
3,793,595	-	rfaF	b3620	0.61	+2.3
3,793,600	+	rfaF	b3620	0.62	+0.8
3,793,663	+	rfaF	b3620	0.68	-1.2
3,793,725	-	rfaF	b3620	0.74	-0.6
3,793,725	-	rfaF	b3620	0.74	+4.0
3,793,734	-	rfaF	b3620	0.75	+1.7
3,793,734	-	rfaF	b3620	0.75	+1.6
3,793,765	-	rfaF	b3620	0.78	-2.3
3,793,775	+	rfaF	b3620	0.79	+2.8
3,793,775	+	rfaF	b3620	0.79	+0.6
3,793,813	+	rfaF	b3620	0.82	+2.8
3,793,813	+				-1.0
3,793,821	-	rfaF	b3620	0.83	-1.3
3,793,857	-	rfaF	b3620	0.86	+0.3
3,793,857	-	rfaF	b3620	0.86	+1.3
3,793,916	+				+0.8
3,793,921	-				+0.7
3,793,921	-				+1.4
3,793,926	-				+1.4
3,793,984	-				-0.6
3,794,047	-				-1.6
3,794,050	+				+2.4
3,794,050	+				+0.3
3,794,054	+				-0.8
3,794,054	+				-0.9
3,794,107	+	rfaC	b3621	0.11	+0.7
3,794,120	+	rfaC	b3621	0.12	+1.3
3,794,120	+	rfaC	b3621	0.12	+0.6
3,794,128	-	rfaC	b3621	0.13	+0.7
3,794,279	+	rfaC	b3621	0.29	-0.2
3,794,287	-	rfaC	b3621	0.30	+0.9
3,794,322	-	rfaC	b3621	0.33	+0.7
3,794,322	-	rfaC	b3621	0.33	+0.4
3,794,335	-	rfaC	b3621	0.35	+0.4
3,794,335	-	rfaC	b3621	0.35	-0.3
3,794,346	-	rfaC	b3621	0.36	+0.2
3,794,365	+	rfaC	b3621	0.38	+1.1
3,794,365	+	rfaC	b3621	0.38	-0.0
3,794,402	+	rfaC	b3621	0.42	+2.4
3,794,402	+	rfaC	b3621	0.42	+0.4
3,794,428	-	rfaC	b3621	0.44	+1.6
3,794,493	-	rfaC	b3621	0.51	-0.8
3,794,519	+	rfaC	b3621	0.54	+0.9
3,794,520	+	rfaC	b3621	0.54	+2.0
3,794,570	+	rfaC	b3621	0.59	+1.4
3,794,597	+				+3.0
3,794,597	+	rfaC	b3621	0.62	-0.3
3,794,612	+	rfaC	b3621	0.64	-0.0
3,794,713	-	rfaC	b3621	0.74	-0.8
3,794,713	-	rfaC	b3621	0.74	+0.4
3,794,728	+	rfaC	b3621	0.76	-0.2
3,794,728	+	rfaC	b3621	0.76	+1.9
3,794,729	+	rfaC	b3621	0.76	+0.9
3,794,729	+	rfaC	b3621	0.76	+1.4
3,794,730	+	rfaC	b3621	0.76	+0.1
3,794,765	+	rfaC	b3621	0.79	+2.6
3,794,765	+	rfaC	b3621	0.79	-1.6
3,794,765	+	rfaC	b3621	0.79	+1.3
3,794,765	+	rfaC	b3621	0.79	+2.9
3,794,773	+	rfaC	b3621	0.80	-1.0
3,794,773	+	rfaC	b3621	0.80	+0.7
3,794,778	+	rfaC	b3621	0.81	-2.0
3,794,781	+	rfaC	b3621	0.81	+1.8
3,794,809	+	rfaC	b3621	0.84	+2.0
3,794,809	+	rfaC	b3621	0.84	-1.0
3,794,809	+	rfaC	b3621	0.84	-0.3
3,794,814	+	rfaC	b3621	0.85	-0.4
3,794,814	+	rfaC	b3621	0.85	-0.6
3,794,814	+	rfaC	b3621	0.85	+1.6
3,794,814	+	rfaC	b3621	0.85	+0.4
3,794,820	+	rfaC	b3621	0.85	+0.8
3,794,820	+	rfaC	b3621	0.85	+0.4
3,794,860	+	rfaC	b3621	0.89	+1.7
3,794,860	+	rfaC	b3621	0.89	-1.2
3,794,867	+				-0.3
3,794,867	+				-0.2
3,794,867	+				+2.7
3,794,867	+				+0.8
3,794,867	+				+0.7
3,794,868	+				+0.7
3,794,868	+				+1.0
3,794,868	+				+1.6
3,794,868	+				-1.7
3,794,877	+				-0.0
3,794,879	+				+1.0
3,794,879	+				+1.3
3,794,879	+				-1.3
3,794,886	+				-1.3
3,794,886	+				+1.0
3,794,886	+				+2.0
3,794,887	+				+0.8
3,794,887	+				+0.7
3,794,887	+				+1.9
3,794,887	+				+2.3
3,794,887	+				-0.6
3,794,887	+				+0.4
3,794,887	+				-0.1
3,794,887	+				+2.0
3,794,887	+				+0.5
3,794,887	+				+0.9
3,794,887	+				+0.8
3,794,887	+				+0.1
3,794,887	+				-0.5
3,794,889	+				+0.6
3,794,889	+				-0.2
3,794,889	+				-0.7
3,794,889	+				-0.0
3,794,891	+				-0.1
3,794,917	+				-1.0
3,794,917	+				+1.4
3,794,917	+				+0.8
3,794,919	+				+0.9
3,794,927	+				+0.8
3,794,938	+				+0.4
3,794,943	-				+2.0
3,794,953	+				+0.7
3,795,003	+				+1.0
3,795,003	+				-1.0
3,795,003	+				-0.5
3,795,005	+				-0.3
3,795,146	-	rfaL	b3622	0.14	+2.0
3,795,275	-	rfaL	b3622	0.24	+2.0
3,795,373	+	rfaL	b3622	0.32	+2.0
3,795,423	+	rfaL	b3622	0.36	+2.0
3,795,423	+	rfaL	b3622	0.36	+2.0
3,795,463	+	rfaL	b3622	0.39	+2.0
3,795,463	+	rfaL	b3622	0.39	+2.0
3,795,472	-	rfaL	b3622	0.40	+2.0
3,795,474	+	rfaL	b3622	0.40	+2.0
3,795,489	+	rfaL	b3622	0.41	+2.0
3,795,489	+	rfaL	b3622	0.41	+2.0
3,795,489	+	rfaL	b3622	0.41	+1.0
3,795,494	+	rfaL	b3622	0.42	+2.0
3,795,494	+	rfaL	b3622	0.42	+2.0
3,795,514	+	rfaL	b3622	0.43	+2.0
3,795,521	-	rfaL	b3622	0.44	+2.0
3,795,522	-	rfaL	b3622	0.44	+2.0
3,795,597	+	rfaL	b3622	0.50	+2.0
3,795,609	+	rfaL	b3622	0.51	+2.0
3,795,609	+	rfaL	b3622	0.51	+2.0
3,795,627	-	rfaL	b3622	0.52	+2.0
3,795,628	-	rfaL	b3622	0.52	+2.0
3,795,706	-	rfaL	b3622	0.58	+2.0
3,795,747	-	rfaL	b3622	0.62	+2.0
3,795,773	-	rfaL	b3622	0.64	+2.0
3,795,792	-	rfaL	b3622	0.65	+2.0
3,795,792	-	rfaL	b3622	0.65	+2.0
3,795,794	+	rfaL	b3622	0.65	+2.0
3,795,801	-	rfaL	b3622	0.66	+0.4
3,795,801	-	rfaL	b3622	0.66	+2.0
3,795,818	+	rfaL	b3622	0.67	+2.0
3,795,835	-	rfaL	b3622	0.69	+2.0
3,795,835	-	rfaL	b3622	0.69	+2.0
3,795,835	-	rfaL	b3622	0.69	+2.0
3,795,835	-	rfaL	b3622	0.69	+2.0
3,795,841	-	rfaL	b3622	0.69	+2.0
3,795,893	+	rfaL	b3622	0.73	+2.0
3,795,901	-	rfaL	b3622	0.74	+2.0
3,795,901	-	rfaL	b3622	0.74	+2.0
3,795,901	-	rfaL	b3622	0.74	+2.0
3,795,936	+	rfaL	b3622	0.77	-0.0
3,795,938	+	rfaL	b3622	0.77	+2.0
3,795,939	+	rfaL	b3622	0.77	+2.0
3,795,939	+	rfaL	b3622	0.77	+2.0
3,795,939	+	rfaL	b3622	0.77	+2.0
3,795,939	+	rfaL	b3622	0.77	+1.0
3,795,939	+	rfaL	b3622	0.77	+0.4
3,795,942	-	rfaL	b3622	0.77	+1.0
3,795,943	+	rfaL	b3622	0.77	+0.4
3,795,944	+	rfaL	b3622	0.77	+2.0
3,795,944	+	rfaL	b3622	0.77	+2.0
3,795,944	+	rfaL	b3622	0.77	+2.0
3,795,944	+	rfaL	b3622	0.77	+2.0
3,795,944	+	rfaL	b3622	0.77	+2.0
3,795,945	+	rfaL	b3622	0.77	+0.7
3,795,945	+	rfaL	b3622	0.77	+1.3
3,795,950	+	rfaL	b3622	0.78	-0.3
3,795,950	+	rfaL	b3622	0.78	+2.0
3,795,955	+	rfaL	b3622	0.78	+2.0
3,795,955	+	rfaL	b3622	0.78	+2.0