Strain Fitness in Escherichia coli BW25113 around b3199

Experiment: Cholinium hydrogen phosphate 10 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdsD and kdsC are separated by 20 nucleotideskdsC and yrbK overlap by 4 nucleotidesyrbK and yhbN overlap by 32 nucleotidesyhbN and yhbG are separated by 6 nucleotides b3197: kdsD - D-arabinose 5-phosphate isomerase (NCBI), at 3,339,288 to 3,340,274 kdsD b3198: kdsC - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI), at 3,340,295 to 3,340,861 kdsC b3199: yrbK - hypothetical protein (NCBI), at 3,340,858 to 3,341,433 yrbK b3200: yhbN - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 3,341,402 to 3,341,959 yhbN b3201: yhbG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 3,341,966 to 3,342,691 yhbG Position (kb) 3340 3341 3342Strain fitness (log2 ratio) -1 0 1 2 3at 3339.864 kb on + strand, within kdsDat 3339.868 kb on - strand, within kdsDat 3339.876 kb on + strand, within kdsDat 3339.879 kb on - strand, within kdsDat 3339.879 kb on - strand, within kdsDat 3339.924 kb on + strand, within kdsDat 3339.974 kb on + strand, within kdsDat 3340.032 kb on + strand, within kdsDat 3340.105 kb on - strand, within kdsDat 3340.129 kb on + strand, within kdsDat 3340.129 kb on + strand, within kdsDat 3340.203 kb on + strandat 3340.203 kb on + strandat 3340.231 kb on + strandat 3340.269 kb on + strandat 3340.326 kb on + strandat 3340.326 kb on + strandat 3340.326 kb on + strandat 3340.374 kb on + strand, within kdsCat 3340.374 kb on + strand, within kdsCat 3340.391 kb on + strand, within kdsCat 3340.391 kb on + strand, within kdsCat 3340.736 kb on + strand, within kdsCat 3341.218 kb on + strand, within yrbKat 3341.218 kb on + strand, within yrbKat 3341.256 kb on + strand, within yrbKat 3341.256 kb on + strand, within yrbKat 3341.388 kb on + strandat 3341.388 kb on + strandat 3341.944 kb on + strandat 3341.944 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholinium hydrogen phosphate 10 vol%
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3,339,864 + kdsD b3197 0.58 +0.4
3,339,868 - kdsD b3197 0.59 +0.0
3,339,876 + kdsD b3197 0.60 +0.1
3,339,879 - kdsD b3197 0.60 +0.2
3,339,879 - kdsD b3197 0.60 -0.5
3,339,924 + kdsD b3197 0.64 -0.6
3,339,974 + kdsD b3197 0.70 +0.2
3,340,032 + kdsD b3197 0.75 +0.1
3,340,105 - kdsD b3197 0.83 +2.8
3,340,129 + kdsD b3197 0.85 +0.3
3,340,129 + kdsD b3197 0.85 +0.9
3,340,203 + +0.4
3,340,203 + +0.1
3,340,231 + -0.5
3,340,269 + +0.3
3,340,326 + +0.5
3,340,326 + +0.4
3,340,326 + -0.1
3,340,374 + kdsC b3198 0.14 -0.1
3,340,374 + kdsC b3198 0.14 +1.0
3,340,391 + kdsC b3198 0.17 +1.2
3,340,391 + kdsC b3198 0.17 -0.4
3,340,736 + kdsC b3198 0.78 -0.9
3,341,218 + yrbK b3199 0.62 +1.0
3,341,218 + yrbK b3199 0.62 +2.8
3,341,256 + yrbK b3199 0.69 +1.0
3,341,256 + yrbK b3199 0.69 -0.0
3,341,388 + +1.0
3,341,388 + -0.0
3,341,944 + +0.9
3,341,944 + +0.0

Or see this region's nucleotide sequence