Experiment: Cholinium hydrogen phosphate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and ssuB are separated by 42 nucleotides ssuB and ycbM overlap by 4 nucleotides ycbM and ssuD are separated by 10 nucleotides
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
Position (kb) 993
994
995 Strain fitness (log2 ratio) -2
-1
0
1 at 992.402 kb on - strand at 992.438 kb on + strand at 992.582 kb on - strand, within ssuB at 992.676 kb on + strand, within ssuB at 992.681 kb on + strand, within ssuB at 992.712 kb on + strand, within ssuB at 992.928 kb on - strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.056 kb on - strand, within ssuB at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand at 993.526 kb on + strand, within ycbM at 993.729 kb on - strand, within ycbM at 993.913 kb on + strand, within ycbM at 994.024 kb on + strand at 994.033 kb on - strand at 994.089 kb on + strand at 994.089 kb on + strand at 994.335 kb on - strand, within ssuD at 994.356 kb on + strand, within ssuD at 994.364 kb on - strand, within ssuD at 994.597 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.723 kb on + strand, within ssuD at 994.869 kb on + strand, within ssuD at 994.869 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.962 kb on + strand, within ssuD at 995.043 kb on - strand, within ssuD
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium hydrogen phosphate 10 vol% remove 992,402 - -0.5 992,438 + +1.2 992,582 - ssuB b0933 0.11 -0.7 992,676 + ssuB b0933 0.23 +0.4 992,681 + ssuB b0933 0.24 +0.4 992,712 + ssuB b0933 0.28 -0.7 992,928 - ssuB b0933 0.56 -2.1 993,003 + ssuB b0933 0.65 -0.2 993,003 + ssuB b0933 0.65 +0.3 993,056 - ssuB b0933 0.72 -0.6 993,078 - ssuB b0933 0.75 +1.3 993,103 + ssuB b0933 0.79 +1.0 993,239 - -0.2 993,526 + ycbM b0934 0.33 +1.5 993,729 - ycbM b0934 0.59 +0.8 993,913 + ycbM b0934 0.82 -1.0 994,024 + +0.1 994,033 - -1.8 994,089 + +0.1 994,089 + +0.5 994,335 - ssuD b0935 0.23 -0.9 994,356 + ssuD b0935 0.25 -0.1 994,364 - ssuD b0935 0.26 +0.3 994,597 - ssuD b0935 0.46 -1.2 994,710 - ssuD b0935 0.56 +0.3 994,710 - ssuD b0935 0.56 -0.4 994,723 + ssuD b0935 0.57 -0.6 994,869 + ssuD b0935 0.70 +0.4 994,869 - ssuD b0935 0.70 -0.8 994,900 - ssuD b0935 0.73 -0.5 994,900 - ssuD b0935 0.73 +0.3 994,962 + ssuD b0935 0.78 +0.3 995,043 - ssuD b0935 0.85 +0.1
Or see this region's nucleotide sequence