Experiment: Cholinium hydrogen phosphate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhA and sdhB are separated by 15 nucleotides sdhB and sucA are separated by 300 nucleotides
b0723: sdhA - succinate dehydrogenase flavoprotein subunit (NCBI), at 755,130 to 756,896
sdhA
b0724: sdhB - succinate dehydrogenase, FeS subunit (NCBI), at 756,912 to 757,628
sdhB
b0726: sucA - alpha-ketoglutarate decarboxylase (NCBI), at 757,929 to 760,730
sucA
Position (kb) 756
757
758 Strain fitness (log2 ratio) -1
0
1
2 at 755.961 kb on - strand, within sdhA at 756.184 kb on + strand, within sdhA at 756.390 kb on - strand, within sdhA at 756.390 kb on - strand, within sdhA at 756.455 kb on - strand, within sdhA at 756.455 kb on - strand, within sdhA at 756.500 kb on + strand, within sdhA at 756.500 kb on + strand, within sdhA at 756.642 kb on + strand, within sdhA at 756.677 kb on + strand, within sdhA at 756.680 kb on - strand, within sdhA at 756.685 kb on - strand at 756.839 kb on + strand at 756.856 kb on - strand at 757.053 kb on + strand, within sdhB at 757.138 kb on - strand, within sdhB at 757.143 kb on - strand, within sdhB at 757.184 kb on - strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.229 kb on - strand, within sdhB at 757.233 kb on + strand, within sdhB at 757.241 kb on - strand, within sdhB at 757.381 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.488 kb on + strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.536 kb on + strand at 757.536 kb on + strand, within sdhB at 757.536 kb on - strand, within sdhB at 757.544 kb on + strand, within sdhB at 757.544 kb on - strand, within sdhB at 757.566 kb on - strand at 757.566 kb on - strand at 757.673 kb on + strand at 757.681 kb on - strand at 757.703 kb on + strand at 757.713 kb on - strand at 757.713 kb on - strand at 757.791 kb on + strand at 757.929 kb on - strand at 758.303 kb on + strand, within sucA at 758.313 kb on + strand, within sucA
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium hydrogen phosphate 10 vol% remove 755,961 - sdhA b0723 0.47 +0.2 756,184 + sdhA b0723 0.60 +0.2 756,390 - sdhA b0723 0.71 +1.3 756,390 - sdhA b0723 0.71 +1.4 756,455 - sdhA b0723 0.75 +1.0 756,455 - sdhA b0723 0.75 +1.4 756,500 + sdhA b0723 0.78 -1.5 756,500 + sdhA b0723 0.78 +1.1 756,642 + sdhA b0723 0.86 -0.0 756,677 + sdhA b0723 0.88 +1.2 756,680 - sdhA b0723 0.88 +0.8 756,685 - +2.0 756,839 + -0.6 756,856 - +1.8 757,053 + sdhB b0724 0.20 +1.5 757,138 - sdhB b0724 0.32 +1.6 757,143 - sdhB b0724 0.32 -1.6 757,184 - sdhB b0724 0.38 -0.3 757,198 + sdhB b0724 0.40 -0.1 757,198 + sdhB b0724 0.40 -0.1 757,229 - sdhB b0724 0.44 +0.9 757,233 + sdhB b0724 0.45 +0.6 757,241 - sdhB b0724 0.46 +1.0 757,381 - sdhB b0724 0.65 +2.3 757,392 - sdhB b0724 0.67 +1.5 757,392 - sdhB b0724 0.67 +0.5 757,488 + sdhB b0724 0.80 +0.0 757,517 - sdhB b0724 0.84 +0.3 757,517 - sdhB b0724 0.84 +1.5 757,536 + -0.6 757,536 + sdhB b0724 0.87 +1.1 757,536 - sdhB b0724 0.87 +0.9 757,544 + sdhB b0724 0.88 -0.6 757,544 - sdhB b0724 0.88 +1.0 757,566 - +1.8 757,566 - +0.9 757,673 + +0.5 757,681 - +0.6 757,703 + +0.2 757,713 - -0.6 757,713 - +0.3 757,791 + +0.8 757,929 - +0.1 758,303 + sucA b0726 0.13 +1.6 758,313 + sucA b0726 0.14 +1.8
Or see this region's nucleotide sequence