Experiment: Cholinium hydrogen phosphate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltA and sdhC are separated by 708 nucleotides sdhC and sdhD overlap by 7 nucleotides sdhD and sdhA overlap by 1 nucleotides
b0720: gltA - citrate synthase (NCBI), at 752,408 to 753,691
gltA
b0721: sdhC - succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI), at 754,400 to 754,789
sdhC
b0722: sdhD - succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI), at 754,783 to 755,130
sdhD
b0723: sdhA - succinate dehydrogenase flavoprotein subunit (NCBI), at 755,130 to 756,896
sdhA
Position (kb) 754
755 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 753.449 kb on - strand, within gltA at 753.567 kb on - strand at 753.621 kb on + strand at 753.621 kb on + strand at 753.688 kb on - strand at 753.813 kb on + strand at 753.846 kb on + strand at 753.944 kb on + strand at 754.017 kb on - strand at 754.126 kb on + strand at 754.174 kb on + strand at 754.209 kb on + strand at 754.305 kb on + strand at 754.305 kb on + strand at 754.385 kb on + strand at 754.477 kb on - strand, within sdhC at 754.507 kb on - strand, within sdhC at 754.512 kb on + strand, within sdhC at 754.550 kb on + strand, within sdhC at 754.636 kb on - strand, within sdhC at 754.663 kb on - strand, within sdhC at 754.715 kb on + strand, within sdhC at 754.759 kb on - strand at 754.775 kb on - strand at 754.802 kb on + strand at 754.809 kb on + strand at 754.909 kb on - strand, within sdhD at 754.950 kb on + strand, within sdhD at 754.966 kb on - strand, within sdhD at 754.977 kb on + strand, within sdhD at 754.990 kb on + strand, within sdhD at 755.014 kb on - strand, within sdhD at 755.019 kb on + strand, within sdhD at 755.045 kb on + strand, within sdhD at 755.083 kb on - strand, within sdhD at 755.098 kb on + strand at 755.115 kb on + strand at 755.115 kb on + strand at 755.116 kb on - strand at 755.290 kb on + strand at 755.301 kb on + strand at 755.320 kb on + strand, within sdhA at 755.323 kb on - strand, within sdhA at 755.344 kb on - strand, within sdhA at 755.353 kb on + strand, within sdhA at 755.366 kb on - strand, within sdhA at 755.368 kb on - strand, within sdhA at 755.382 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.481 kb on - strand, within sdhA at 755.491 kb on - strand, within sdhA at 755.554 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.564 kb on + strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.572 kb on + strand, within sdhA at 755.625 kb on + strand, within sdhA at 755.626 kb on - strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.663 kb on + strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.697 kb on - strand, within sdhA at 755.711 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium hydrogen phosphate 10 vol% remove 753,449 - gltA b0720 0.81 +1.7 753,567 - +0.1 753,621 + -0.3 753,621 + -1.1 753,688 - -1.3 753,813 + +1.5 753,846 + -0.5 753,944 + +0.2 754,017 - -0.3 754,126 + +0.1 754,174 + -0.1 754,209 + +0.1 754,305 + +3.0 754,305 + -0.7 754,385 + +0.0 754,477 - sdhC b0721 0.20 +1.3 754,507 - sdhC b0721 0.27 +1.3 754,512 + sdhC b0721 0.29 +0.2 754,550 + sdhC b0721 0.38 +0.2 754,636 - sdhC b0721 0.61 +1.3 754,663 - sdhC b0721 0.67 +0.6 754,715 + sdhC b0721 0.81 -0.2 754,759 - +1.4 754,775 - +1.5 754,802 + +1.6 754,809 + +1.2 754,909 - sdhD b0722 0.36 +1.3 754,950 + sdhD b0722 0.48 -0.4 754,966 - sdhD b0722 0.53 +0.3 754,977 + sdhD b0722 0.56 +1.0 754,990 + sdhD b0722 0.59 -1.5 755,014 - sdhD b0722 0.66 +1.5 755,019 + sdhD b0722 0.68 +0.8 755,045 + sdhD b0722 0.75 +0.2 755,083 - sdhD b0722 0.86 +1.1 755,098 + +0.4 755,115 + +0.4 755,115 + -0.1 755,116 - +1.1 755,290 + +0.3 755,301 + +0.4 755,320 + sdhA b0723 0.11 -0.3 755,323 - sdhA b0723 0.11 +1.4 755,344 - sdhA b0723 0.12 +1.4 755,353 + sdhA b0723 0.13 +0.4 755,366 - sdhA b0723 0.13 +0.7 755,368 - sdhA b0723 0.13 +1.3 755,382 + sdhA b0723 0.14 +1.1 755,424 + sdhA b0723 0.17 +0.6 755,424 + sdhA b0723 0.17 +1.2 755,461 - sdhA b0723 0.19 +0.2 755,461 - sdhA b0723 0.19 +0.9 755,481 - sdhA b0723 0.20 +1.1 755,491 - sdhA b0723 0.20 +1.4 755,554 + sdhA b0723 0.24 +0.2 755,562 + sdhA b0723 0.24 +0.8 755,562 + sdhA b0723 0.24 +0.5 755,562 + sdhA b0723 0.24 +0.3 755,564 + sdhA b0723 0.25 +0.3 755,570 - sdhA b0723 0.25 +0.8 755,570 - sdhA b0723 0.25 +1.0 755,570 - sdhA b0723 0.25 +0.9 755,572 + sdhA b0723 0.25 -1.6 755,625 + sdhA b0723 0.28 +0.7 755,626 - sdhA b0723 0.28 +0.6 755,658 + sdhA b0723 0.30 +0.1 755,658 + sdhA b0723 0.30 +0.7 755,663 + sdhA b0723 0.30 -0.6 755,676 - sdhA b0723 0.31 +1.1 755,676 - sdhA b0723 0.31 +1.1 755,676 - sdhA b0723 0.31 +1.0 755,697 - sdhA b0723 0.32 +0.2 755,711 + sdhA b0723 0.33 -0.1 755,759 + sdhA b0723 0.36 -2.7 755,759 + sdhA b0723 0.36 -0.8 755,764 + sdhA b0723 0.36 +0.1 755,764 + sdhA b0723 0.36 -0.2
Or see this region's nucleotide sequence