Strain Fitness in Escherichia coli BW25113 around b0143

Experiment: Cholinium hydrogen phosphate 10 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyadN and folK are separated by 369 nucleotidesfolK and pcnB overlap by 4 nucleotidespcnB and yadB are separated by 59 nucleotides b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883 yadN b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732 folK b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126 pcnB b0144: yadB - glutamyl-Q tRNA(Asp) synthetase (RefSeq), at 159,186 to 160,112 yadB Position (kb) 157 158 159 160Strain fitness (log2 ratio) -2 -1 0 1 2at 156.734 kb on + strand, within yadNat 156.735 kb on + strand, within yadNat 156.735 kb on + strand, within yadNat 156.744 kb on + strand, within yadNat 156.755 kb on + strand, within yadNat 156.757 kb on + strand, within yadNat 156.759 kb on + strand, within yadNat 156.786 kb on + strand, within yadNat 156.789 kb on + strand, within yadNat 156.793 kb on + strand, within yadNat 156.804 kb on + strand, within yadNat 156.823 kb on + strand, within yadNat 156.823 kb on + strand, within yadNat 156.825 kb on + strandat 156.827 kb on + strandat 156.827 kb on + strandat 156.833 kb on - strandat 156.834 kb on + strandat 156.849 kb on + strandat 156.849 kb on + strandat 156.849 kb on + strandat 156.850 kb on + strandat 156.854 kb on + strandat 156.854 kb on + strandat 157.218 kb on - strandat 157.279 kb on - strandat 157.291 kb on - strandat 157.301 kb on - strand, within folKat 157.745 kb on + strandat 157.870 kb on - strand, within pcnBat 157.906 kb on - strand, within pcnBat 157.906 kb on - strand, within pcnBat 157.923 kb on - strand, within pcnBat 158.143 kb on + strand, within pcnBat 158.156 kb on + strand, within pcnBat 158.227 kb on - strand, within pcnBat 158.249 kb on - strand, within pcnBat 158.249 kb on - strand, within pcnBat 158.312 kb on - strand, within pcnBat 158.342 kb on - strand, within pcnBat 158.378 kb on - strand, within pcnBat 158.400 kb on + strand, within pcnBat 158.408 kb on - strand, within pcnBat 158.408 kb on - strand, within pcnBat 158.426 kb on - strand, within pcnBat 158.442 kb on - strand, within pcnBat 158.442 kb on - strand, within pcnBat 158.447 kb on - strand, within pcnBat 158.516 kb on + strand, within pcnBat 158.522 kb on - strand, within pcnBat 158.759 kb on - strand, within pcnBat 158.759 kb on - strand, within pcnBat 158.759 kb on - strand, within pcnBat 158.864 kb on - strand, within pcnBat 159.038 kb on - strandat 159.093 kb on - strandat 159.093 kb on - strandat 159.133 kb on + strandat 159.347 kb on - strand, within yadBat 159.347 kb on - strand, within yadBat 159.352 kb on - strand, within yadBat 159.352 kb on - strand, within yadBat 159.581 kb on - strand, within yadBat 159.655 kb on + strand, within yadBat 159.699 kb on + strand, within yadBat 159.722 kb on - strand, within yadBat 159.722 kb on - strand, within yadBat 159.743 kb on - strand, within yadBat 159.754 kb on - strand, within yadBat 159.754 kb on - strand, within yadBat 159.770 kb on + strand, within yadBat 159.770 kb on + strand, within yadBat 159.803 kb on + strand, within yadBat 159.808 kb on + strand, within yadBat 159.905 kb on - strand, within yadBat 159.947 kb on + strand, within yadBat 160.046 kb on + strandat 160.068 kb on + strandat 160.068 kb on - strandat 160.068 kb on - strandat 160.070 kb on - strandat 160.078 kb on + strandat 160.078 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholinium hydrogen phosphate 10 vol%
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156,734 + yadN b0141 0.74 -2.0
156,735 + yadN b0141 0.75 -0.4
156,735 + yadN b0141 0.75 -0.4
156,744 + yadN b0141 0.76 -0.7
156,755 + yadN b0141 0.78 +1.9
156,757 + yadN b0141 0.78 +1.6
156,759 + yadN b0141 0.79 -0.2
156,786 + yadN b0141 0.83 +1.6
156,789 + yadN b0141 0.84 -2.2
156,793 + yadN b0141 0.84 -0.6
156,804 + yadN b0141 0.86 +1.9
156,823 + yadN b0141 0.90 +1.6
156,823 + yadN b0141 0.90 -1.2
156,825 + +0.2
156,827 + +0.6
156,827 + +1.8
156,833 - +1.6
156,834 + +0.6
156,849 + +0.0
156,849 + +0.4
156,849 + +2.0
156,850 + +0.5
156,854 + +1.4
156,854 + +0.0
157,218 - +1.6
157,279 - -1.4
157,291 - -1.0
157,301 - folK b0142 0.10 +1.0
157,745 + -0.0
157,870 - pcnB b0143 0.10 +0.5
157,906 - pcnB b0143 0.13 +0.0
157,906 - pcnB b0143 0.13 +0.6
157,923 - pcnB b0143 0.14 -0.2
158,143 + pcnB b0143 0.30 +1.6
158,156 + pcnB b0143 0.31 +0.7
158,227 - pcnB b0143 0.36 +0.0
158,249 - pcnB b0143 0.37 +0.0
158,249 - pcnB b0143 0.37 -0.1
158,312 - pcnB b0143 0.42 +0.3
158,342 - pcnB b0143 0.44 +1.3
158,378 - pcnB b0143 0.46 +0.7
158,400 + pcnB b0143 0.48 -0.7
158,408 - pcnB b0143 0.49 -1.0
158,408 - pcnB b0143 0.49 +0.5
158,426 - pcnB b0143 0.50 +0.0
158,442 - pcnB b0143 0.51 -0.7
158,442 - pcnB b0143 0.51 +2.2
158,447 - pcnB b0143 0.51 +1.8
158,516 + pcnB b0143 0.56 +0.6
158,522 - pcnB b0143 0.57 -1.0
158,759 - pcnB b0143 0.74 +0.9
158,759 - pcnB b0143 0.74 +0.8
158,759 - pcnB b0143 0.74 +1.0
158,864 - pcnB b0143 0.81 +0.4
159,038 - +0.6
159,093 - +0.6
159,093 - +1.1
159,133 + +0.3
159,347 - yadB b0144 0.17 -0.0
159,347 - yadB b0144 0.17 -1.0
159,352 - yadB b0144 0.18 +0.6
159,352 - yadB b0144 0.18 +0.0
159,581 - yadB b0144 0.43 +0.2
159,655 + yadB b0144 0.51 -1.4
159,699 + yadB b0144 0.55 -0.1
159,722 - yadB b0144 0.58 -0.2
159,722 - yadB b0144 0.58 -0.4
159,743 - yadB b0144 0.60 -0.3
159,754 - yadB b0144 0.61 -0.5
159,754 - yadB b0144 0.61 -0.6
159,770 + yadB b0144 0.63 -0.0
159,770 + yadB b0144 0.63 -0.2
159,803 + yadB b0144 0.67 +0.3
159,808 + yadB b0144 0.67 +0.0
159,905 - yadB b0144 0.78 +0.7
159,947 + yadB b0144 0.82 -0.6
160,046 + +0.1
160,068 + -0.5
160,068 - -0.7
160,068 - -1.1
160,070 - +0.4
160,078 + -1.2
160,078 + +0.3

Or see this region's nucleotide sequence