Strain Fitness in Escherichia coli BW25113 around b4481

Experiment: Cholinium glutamate 10 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Cholinium glutamate 10 vol%
3,974,569	+	wzxE	b3792	0.21	-0.2
3,974,630	-	wzxE	b3792	0.26	-0.8
3,974,630	-	wzxE	b3792	0.26	+0.6
3,974,641	+	wzxE	b3792	0.27	+1.0
3,974,641	+	wzxE	b3792	0.27	+2.0
3,974,644	+	wzxE	b3792	0.27	-0.6
3,974,687	+	wzxE	b3792	0.31	+1.5
3,974,687	+	wzxE	b3792	0.31	+2.0
3,974,688	+	wzxE	b3792	0.31	+0.2
3,974,693	+	wzxE	b3792	0.31	+1.0
3,974,706	-	wzxE	b3792	0.32	+0.2
3,974,708	-	wzxE	b3792	0.33	+0.2
3,974,789	+	wzxE	b3792	0.39	-1.4
3,974,795	-	wzxE	b3792	0.39	-0.5
3,974,820	-	wzxE	b3792	0.41	-0.8
3,974,879	+	wzxE	b3792	0.46	+2.5
3,974,882	-	wzxE	b3792	0.46	-0.5
3,974,933	+	wzxE	b3792	0.51	-1.7
3,974,935	-	wzxE	b3792	0.51	+1.7
3,974,988	+	wzxE	b3792	0.55	+1.0
3,975,038	-	wzxE	b3792	0.59	+0.2
3,975,041	-	wzxE	b3792	0.59	+4.3
3,975,041	-	wzxE	b3792	0.59	-0.6
3,975,043	-				+2.5
3,975,043	-	wzxE	b3792	0.59	+1.0
3,975,053	-	wzxE	b3792	0.60	+2.0
3,975,053	-	wzxE	b3792	0.60	-0.6
3,975,076	+	wzxE	b3792	0.62	-0.4
3,975,088	-	wzxE	b3792	0.63	-0.2
3,975,118	+	wzxE	b3792	0.65	-1.1
3,975,118	+	wzxE	b3792	0.65	+1.4
3,975,118	+	wzxE	b3792	0.65	-0.2
3,975,126	-	wzxE	b3792	0.66	-0.0
3,975,144	-	wzxE	b3792	0.67	-0.2
3,975,162	+	wzxE	b3792	0.69	-0.8
3,975,174	+	wzxE	b3792	0.70	+0.4
3,975,177	-	wzxE	b3792	0.70	+0.2
3,975,186	-	wzxE	b3792	0.71	-0.8
3,975,223	+	wzxE	b3792	0.74	+1.0
3,975,322	-	wzxE	b3792	0.82	-1.7
3,975,333	-	wzxE	b3792	0.82	+2.0
3,975,353	-	wzxE	b3792	0.84	-0.8
3,975,362	-	wzxE	b3792	0.85	+1.4
3,975,364	+	wzxE	b3792	0.85	+1.0
3,975,364	+	wzxE	b3792	0.85	+0.4
3,975,393	-	wzxE	b3792	0.87	+2.7
3,975,393	-	wzxE	b3792	0.87	+0.2
3,975,435	-				+0.8
3,975,435	-				+2.0
3,975,447	-				+1.1
3,975,447	-				+1.0
3,975,456	+				-1.1
3,975,462	-				+1.8
3,975,462	-				-0.6
3,975,476	+				+2.0
3,975,476	+				-0.8
3,975,479	+				-1.5
3,975,498	-				-1.2
3,975,498	-				-1.4
3,975,544	+				-0.7
3,975,546	+				+0.4
3,975,549	+				-0.4
3,975,549	+				+0.2
3,975,549	+				+0.0
3,975,551	+				-0.2
3,975,554	+				-0.7
3,975,554	+				-0.0
3,975,554	+				+1.0
3,975,562	-				-0.2
3,975,562	-				-1.3
3,975,562	-				-0.4
3,975,572	+				-1.0
3,975,572	+				+0.5
3,975,585	-				+0.6
3,975,585	-				+2.9
3,975,590	-				+2.1
3,975,665	+	rffT	b4481	0.11	+1.1
3,975,679	+	rffT	b4481	0.12	+0.4
3,975,679	+	rffT	b4481	0.12	-0.8
3,975,681	-	rffT	b4481	0.12	+0.7
3,975,694	-	rffT	b4481	0.14	+1.0
3,975,715	+	rffT	b4481	0.15	-0.6
3,975,715	+	rffT	b4481	0.15	-0.2
3,975,749	+	rffT	b4481	0.19	+0.9
3,975,760	+	rffT	b4481	0.20	+0.8
3,975,807	+	rffT	b4481	0.24	+0.8
3,975,807	+	rffT	b4481	0.24	+0.4
3,975,815	+	rffT	b4481	0.25	+0.7
3,975,815	-	rffT	b4481	0.25	-0.3
3,975,827	-	rffT	b4481	0.26	+0.8
3,975,827	-	rffT	b4481	0.26	+0.0
3,975,832	-	rffT	b4481	0.26	+0.7
3,975,848	-	rffT	b4481	0.28	-0.0
3,975,849	-	rffT	b4481	0.28	+0.2
3,975,849	-	rffT	b4481	0.28	+2.2
3,975,884	+	rffT	b4481	0.31	-0.2
3,976,051	-	rffT	b4481	0.47	+1.2
3,976,057	+	rffT	b4481	0.47	-0.0
3,976,057	+	rffT	b4481	0.47	-0.4
3,976,148	+	rffT	b4481	0.56	+0.7
3,976,148	+	rffT	b4481	0.56	+1.6
3,976,253	+	rffT	b4481	0.65	-1.1
3,976,317	+	rffT	b4481	0.71	+0.3
3,976,396	+	rffT	b4481	0.79	-0.9
3,976,447	+	rffT	b4481	0.83	+0.0
3,976,480	-	rffT	b4481	0.86	+0.7
3,976,538	+				+0.6
3,976,608	+				-0.8
3,976,608	+				-0.3
3,976,626	-				+1.7