Strain Fitness in Escherichia coli BW25113 around b3622

Experiment: Cholinium glutamate 10 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Cholinium glutamate 10 vol%
3,793,984	-				+1.0
3,794,047	-				+1.7
3,794,050	+				+1.7
3,794,050	+				+0.5
3,794,054	+				-0.9
3,794,054	+				-2.6
3,794,107	+	rfaC	b3621	0.11	+0.6
3,794,120	+	rfaC	b3621	0.12	+0.6
3,794,120	+	rfaC	b3621	0.12	-1.1
3,794,128	-	rfaC	b3621	0.13	+0.6
3,794,279	+	rfaC	b3621	0.29	+0.5
3,794,287	-	rfaC	b3621	0.30	+0.1
3,794,322	-	rfaC	b3621	0.33	-0.4
3,794,322	-	rfaC	b3621	0.33	+0.3
3,794,335	-	rfaC	b3621	0.35	-1.2
3,794,335	-	rfaC	b3621	0.35	-0.4
3,794,346	-	rfaC	b3621	0.36	-0.9
3,794,365	+	rfaC	b3621	0.38	+1.3
3,794,365	+	rfaC	b3621	0.38	-2.4
3,794,402	+	rfaC	b3621	0.42	+2.5
3,794,402	+	rfaC	b3621	0.42	+3.7
3,794,428	-	rfaC	b3621	0.44	-0.3
3,794,493	-	rfaC	b3621	0.51	+1.1
3,794,519	+	rfaC	b3621	0.54	+0.1
3,794,520	+	rfaC	b3621	0.54	+0.6
3,794,570	+	rfaC	b3621	0.59	-0.7
3,794,597	+				+0.9
3,794,597	+	rfaC	b3621	0.62	+0.0
3,794,612	+	rfaC	b3621	0.64	-2.1
3,794,713	-	rfaC	b3621	0.74	-0.9
3,794,713	-	rfaC	b3621	0.74	-0.7
3,794,728	+	rfaC	b3621	0.76	-0.2
3,794,728	+	rfaC	b3621	0.76	+1.1
3,794,729	+	rfaC	b3621	0.76	+0.2
3,794,729	+	rfaC	b3621	0.76	+0.3
3,794,730	+	rfaC	b3621	0.76	-1.0
3,794,765	+	rfaC	b3621	0.79	-0.9
3,794,765	+	rfaC	b3621	0.79	-0.1
3,794,765	+	rfaC	b3621	0.79	+0.6
3,794,765	+	rfaC	b3621	0.79	-0.2
3,794,773	+	rfaC	b3621	0.80	-0.1
3,794,773	+	rfaC	b3621	0.80	-1.6
3,794,778	+	rfaC	b3621	0.81	-2.1
3,794,781	+	rfaC	b3621	0.81	+1.4
3,794,809	+	rfaC	b3621	0.84	+1.3
3,794,809	+	rfaC	b3621	0.84	-0.1
3,794,809	+	rfaC	b3621	0.84	+0.2
3,794,814	+	rfaC	b3621	0.85	+1.4
3,794,814	+	rfaC	b3621	0.85	-0.7
3,794,814	+	rfaC	b3621	0.85	+1.1
3,794,814	+	rfaC	b3621	0.85	-0.4
3,794,820	+	rfaC	b3621	0.85	+1.3
3,794,820	+	rfaC	b3621	0.85	-0.9
3,794,860	+	rfaC	b3621	0.89	+1.9
3,794,860	+	rfaC	b3621	0.89	+1.3
3,794,867	+				-0.4
3,794,867	+				-1.2
3,794,867	+				+0.6
3,794,867	+				+0.7
3,794,867	+				+0.3
3,794,868	+				+0.9
3,794,868	+				+0.7
3,794,868	+				+0.3
3,794,868	+				+0.6
3,794,877	+				+1.5
3,794,879	+				-0.1
3,794,879	+				+0.5
3,794,879	+				+0.2
3,794,886	+				-1.4
3,794,886	+				+1.9
3,794,886	+				+0.8
3,794,887	+				+0.3
3,794,887	+				+1.6
3,794,887	+				-0.5
3,794,887	+				-0.1
3,794,887	+				-1.7
3,794,887	+				+0.3
3,794,887	+				+0.6
3,794,887	+				+0.6
3,794,887	+				+1.7
3,794,887	+				-0.9
3,794,887	+				-0.9
3,794,887	+				+1.0
3,794,887	+				+0.1
3,794,889	+				-0.6
3,794,889	+				+1.1
3,794,889	+				-0.2
3,794,889	+				-2.1
3,794,891	+				+0.2
3,794,917	+				-0.1
3,794,917	+				+1.3
3,794,917	+				+0.5
3,794,919	+				+1.6
3,794,927	+				+0.1
3,794,938	+				+0.5
3,794,943	-				+1.9
3,794,953	+				+2.2
3,795,003	+				+1.9
3,795,003	+				+1.3
3,795,003	+				+0.5
3,795,005	+				+1.6
3,795,146	-	rfaL	b3622	0.14	+1.9
3,795,275	-	rfaL	b3622	0.24	+1.9
3,795,373	+	rfaL	b3622	0.32	+1.9
3,795,423	+	rfaL	b3622	0.36	+1.9
3,795,423	+	rfaL	b3622	0.36	+1.9
3,795,463	+	rfaL	b3622	0.39	+1.9
3,795,463	+	rfaL	b3622	0.39	+1.9
3,795,472	-	rfaL	b3622	0.40	+1.9
3,795,474	+	rfaL	b3622	0.40	+1.9
3,795,489	+	rfaL	b3622	0.41	+1.9
3,795,489	+	rfaL	b3622	0.41	+1.9
3,795,489	+	rfaL	b3622	0.41	+0.9
3,795,494	+	rfaL	b3622	0.42	+1.9
3,795,494	+	rfaL	b3622	0.42	+1.9
3,795,514	+	rfaL	b3622	0.43	+1.9
3,795,521	-	rfaL	b3622	0.44	+1.9
3,795,522	-	rfaL	b3622	0.44	+1.9
3,795,597	+	rfaL	b3622	0.50	+1.9
3,795,609	+	rfaL	b3622	0.51	+1.9
3,795,609	+	rfaL	b3622	0.51	+1.9
3,795,627	-	rfaL	b3622	0.52	+1.9
3,795,628	-	rfaL	b3622	0.52	+1.9
3,795,706	-	rfaL	b3622	0.58	+1.9
3,795,747	-	rfaL	b3622	0.62	+1.9
3,795,773	-	rfaL	b3622	0.64	+1.9
3,795,792	-	rfaL	b3622	0.65	+1.9
3,795,792	-	rfaL	b3622	0.65	+1.9
3,795,794	+	rfaL	b3622	0.65	+1.9
3,795,801	-	rfaL	b3622	0.66	+0.3
3,795,801	-	rfaL	b3622	0.66	+1.9
3,795,818	+	rfaL	b3622	0.67	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,835	-	rfaL	b3622	0.69	+1.9
3,795,841	-	rfaL	b3622	0.69	+1.9
3,795,893	+	rfaL	b3622	0.73	+1.9
3,795,901	-	rfaL	b3622	0.74	+1.9
3,795,901	-	rfaL	b3622	0.74	+1.9
3,795,901	-	rfaL	b3622	0.74	+1.9
3,795,936	+	rfaL	b3622	0.77	-0.1
3,795,938	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+2.9
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,939	+	rfaL	b3622	0.77	+0.9
3,795,939	+	rfaL	b3622	0.77	+1.9
3,795,942	-	rfaL	b3622	0.77	+0.9
3,795,943	+	rfaL	b3622	0.77	+0.3
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,944	+	rfaL	b3622	0.77	+1.9
3,795,945	+	rfaL	b3622	0.77	-0.4
3,795,945	+	rfaL	b3622	0.77	-0.1
3,795,950	+	rfaL	b3622	0.78	+2.4
3,795,950	+	rfaL	b3622	0.78	+1.9
3,795,955	+	rfaL	b3622	0.78	+1.9
3,795,955	+	rfaL	b3622	0.78	+1.9
3,795,970	+	rfaL	b3622	0.79	+1.3
3,795,970	+	rfaL	b3622	0.79	+1.9
3,795,971	+	rfaL	b3622	0.79	+1.9
3,795,971	+	rfaL	b3622	0.79	+0.9
3,795,978	+	rfaL	b3622	0.80	+0.1
3,795,978	-	rfaL	b3622	0.80	-0.7
3,795,981	+	rfaL	b3622	0.80	-0.2
3,795,997	-	rfaL	b3622	0.81	+1.9
3,795,997	-	rfaL	b3622	0.81	+1.9
3,796,005	+	rfaL	b3622	0.82	+1.2
3,796,015	+	rfaL	b3622	0.83	+0.7
3,796,059	+	rfaL	b3622	0.86	+0.3
3,796,107	+				+1.0
3,796,117	+				+0.8
3,796,117	+				-0.1
3,796,121	+				-0.7
3,796,122	+				-0.2
3,796,126	+				+0.1
3,796,130	+				-1.0
3,796,130	+				+1.1
3,796,131	+				-0.5
3,796,135	+				-0.1
3,796,135	+				-0.5
3,796,136	+				-0.7
3,796,139	-				+2.9
3,796,140	+				-1.2
3,796,140	+				+0.8
3,796,140	+				+0.8
3,796,140	+				+0.2
3,796,140	+				-0.4
3,796,140	+				+0.3
3,796,140	+				+1.4
3,796,142	+				+0.3
3,796,146	-				+1.9
3,796,149	+				+0.6
3,796,171	+				+0.1
3,796,171	+				+0.4
3,796,186	+				-1.1
3,796,261	-				+1.9
3,796,265	+				+1.9
3,796,296	-				+0.1
3,796,339	-				+0.4
3,796,339	-				-0.2
3,796,340	-				+0.3
3,796,346	-				-1.9
3,796,346	-				+1.9
3,796,405	-	rfaK	b3623	0.13	-0.1
3,796,481	-	rfaK	b3623	0.20	+0.9
3,796,526	-	rfaK	b3623	0.25	+0.3
3,796,529	-	rfaK	b3623	0.25	+1.9
3,796,529	-	rfaK	b3623	0.25	+0.3
3,796,552	-	rfaK	b3623	0.27	+0.9
3,796,667	-	rfaK	b3623	0.38	+1.9
3,796,667	-	rfaK	b3623	0.38	+0.3
3,796,701	-	rfaK	b3623	0.41	+1.9
3,796,705	-	rfaK	b3623	0.41	+0.9
3,796,830	-	rfaK	b3623	0.53	+1.9
3,796,889	-	rfaK	b3623	0.58	+0.9
3,796,955	+	rfaK	b3623	0.65	+1.9
3,796,981	-	rfaK	b3623	0.67	+1.9
3,796,981	-	rfaK	b3623	0.67	+0.9
3,796,981	-	rfaK	b3623	0.67	+1.9
3,796,991	-	rfaK	b3623	0.68	+0.9
3,796,999	-	rfaK	b3623	0.69	+1.9
3,796,999	-	rfaK	b3623	0.69	+2.9
3,797,015	-	rfaK	b3623	0.70	-1.2
3,797,015	-	rfaK	b3623	0.70	+1.9
3,797,015	-	rfaK	b3623	0.70	+0.6
3,797,015	-	rfaK	b3623	0.70	+2.5
3,797,015	-	rfaK	b3623	0.70	-0.1
3,797,015	-	rfaK	b3623	0.70	+0.9
3,797,017	-	rfaK	b3623	0.70	+1.9
3,797,025	-	rfaK	b3623	0.71	+0.9
3,797,190	-	rfaK	b3623	0.86	+0.9
3,797,190	-	rfaK	b3623	0.86	+1.9