Strain Fitness in Escherichia coli BW25113 around b3448

Experiment: Cholinium glutamate 10 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Cholinium glutamate 10 vol%
3,583,981	+	ggt	b3447	0.50	-0.8
3,583,981	+	ggt	b3447	0.50	-0.7
3,583,993	-	ggt	b3447	0.51	-1.3
3,584,026	-	ggt	b3447	0.53	+0.5
3,584,032	+	ggt	b3447	0.53	-0.2
3,584,032	+	ggt	b3447	0.53	+0.6
3,584,042	-	ggt	b3447	0.54	-0.4
3,584,052	-	ggt	b3447	0.54	+0.5
3,584,367	-	ggt	b3447	0.72	-1.1
3,584,403	-	ggt	b3447	0.75	+0.5
3,584,462	-	ggt	b3447	0.78	+1.2
3,584,552	-	ggt	b3447	0.83	+0.6
3,584,574	-	ggt	b3447	0.84	+0.8
3,584,659	+	ggt	b3447	0.89	-0.1
3,584,659	+	ggt	b3447	0.89	+0.0
3,584,701	-				-1.0
3,584,701	-				-0.1
3,584,735	+				+0.6
3,584,735	+				+0.5
3,584,735	+				+0.2
3,584,741	-				+0.4
3,584,804	+				+0.4
3,584,861	+				+0.3
3,584,867	+				-0.7
3,584,874	+				+0.2
3,584,894	-				-0.5
3,584,901	+				+0.5
3,584,901	+				-0.1
3,584,993	+				+0.0
3,585,047	-	yhhA	b3448	0.18	-0.0
3,585,047	-	yhhA	b3448	0.18	-0.4
3,585,069	+	yhhA	b3448	0.23	+0.5
3,585,099	-	yhhA	b3448	0.30	+0.1
3,585,164	-	yhhA	b3448	0.45	+0.4
3,585,166	+	yhhA	b3448	0.45	+0.7
3,585,166	+	yhhA	b3448	0.45	+0.3
3,585,316	+	yhhA	b3448	0.79	+1.5
3,585,366	-				-0.1
3,585,369	-				-0.6
3,585,383	+				+0.0
3,585,431	+				-0.6
3,585,436	+				-1.4
3,585,436	+				-0.2
3,585,436	+				+0.4
3,585,462	-				-1.0
3,585,485	-	ugpQ	b3449	0.12	+0.4
3,585,485	-	ugpQ	b3449	0.12	+0.4
3,585,503	+	ugpQ	b3449	0.15	-0.4
3,585,508	-	ugpQ	b3449	0.15	+0.0
3,585,508	-	ugpQ	b3449	0.15	-0.3
3,585,531	-	ugpQ	b3449	0.19	-0.0
3,585,547	+	ugpQ	b3449	0.21	+0.2
3,585,673	+	ugpQ	b3449	0.38	+0.4
3,585,673	+	ugpQ	b3449	0.38	+0.1
3,585,702	-	ugpQ	b3449	0.42	+0.3
3,585,702	-	ugpQ	b3449	0.42	+0.2
3,585,838	-	ugpQ	b3449	0.60	+0.9
3,585,838	-	ugpQ	b3449	0.60	-0.0
3,585,859	-	ugpQ	b3449	0.63	+0.1
3,586,093	-				-0.2
3,586,198	-				-0.7
3,586,198	-				+0.1
3,586,312	-	ugpC	b3450	0.17	+0.1
3,586,320	-	ugpC	b3450	0.17	-0.4
3,586,396	+	ugpC	b3450	0.25	+0.2
3,586,396	+	ugpC	b3450	0.25	+0.0