Experiment: Cholinium glutamate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yojL and ompC are separated by 111 nucleotides ompC and micF are separated by 334 nucleotides micF and rcsD are separated by 311 nucleotides
b2214: yojL - predicted thiamine biosynthesis lipoprotein (NCBI), at 2,308,501 to 2,309,556
yojL
b2215: ompC - outer membrane porin protein C (NCBI), at 2,309,668 to 2,310,771
ompC
b4439: micF - regulatory sRNA (NCBI), at 2,311,106 to 2,311,198
micF
b2216: rcsD - phosphotransfer intermediate protein in two-component regulatory system with RcsBC (NCBI), at 2,311,510 to 2,314,182
rcsD
Position (kb)
2309
2310
2311 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2308.745 kb on - strand, within yojL at 2308.784 kb on - strand, within yojL at 2308.796 kb on - strand, within yojL at 2308.916 kb on + strand, within yojL at 2308.916 kb on + strand, within yojL at 2308.964 kb on - strand, within yojL at 2308.964 kb on - strand, within yojL at 2309.070 kb on - strand, within yojL at 2309.100 kb on + strand, within yojL at 2309.123 kb on - strand, within yojL at 2309.286 kb on - strand, within yojL at 2309.286 kb on - strand, within yojL at 2309.711 kb on - strand at 2309.963 kb on + strand, within ompC at 2309.981 kb on + strand, within ompC at 2310.013 kb on + strand, within ompC at 2310.044 kb on + strand, within ompC at 2310.052 kb on - strand, within ompC at 2310.052 kb on - strand at 2310.081 kb on - strand, within ompC at 2310.091 kb on - strand, within ompC at 2310.155 kb on + strand, within ompC at 2310.192 kb on + strand, within ompC at 2310.277 kb on + strand, within ompC at 2310.340 kb on + strand, within ompC at 2310.362 kb on - strand, within ompC at 2310.362 kb on - strand, within ompC at 2310.362 kb on - strand, within ompC at 2310.442 kb on + strand, within ompC at 2310.442 kb on + strand, within ompC at 2310.490 kb on + strand, within ompC at 2310.531 kb on + strand, within ompC at 2310.532 kb on + strand, within ompC at 2310.538 kb on - strand, within ompC at 2310.538 kb on - strand, within ompC at 2310.562 kb on - strand, within ompC at 2310.599 kb on + strand, within ompC at 2310.601 kb on + strand, within ompC at 2310.602 kb on + strand, within ompC at 2310.602 kb on + strand, within ompC at 2310.602 kb on + strand, within ompC at 2310.602 kb on + strand, within ompC at 2310.607 kb on + strand, within ompC at 2310.607 kb on + strand, within ompC at 2310.608 kb on - strand, within ompC at 2310.610 kb on - strand, within ompC at 2310.615 kb on - strand, within ompC at 2310.629 kb on - strand, within ompC at 2310.672 kb on + strand at 2310.680 kb on - strand at 2310.680 kb on - strand at 2310.700 kb on + strand at 2310.700 kb on + strand at 2310.721 kb on + strand at 2310.725 kb on + strand at 2310.734 kb on - strand at 2310.736 kb on + strand at 2310.737 kb on + strand at 2310.743 kb on - strand at 2310.746 kb on + strand at 2310.746 kb on + strand at 2310.748 kb on + strand at 2310.765 kb on - strand at 2310.765 kb on - strand at 2310.787 kb on - strand at 2310.787 kb on - strand at 2310.821 kb on + strand at 2310.824 kb on + strand at 2310.824 kb on - strand at 2310.824 kb on - strand at 2310.824 kb on - strand at 2310.824 kb on - strand at 2310.826 kb on + strand at 2310.829 kb on - strand at 2310.834 kb on + strand at 2310.835 kb on + strand at 2310.835 kb on + strand at 2310.842 kb on - strand at 2310.843 kb on - strand at 2310.883 kb on - strand at 2310.888 kb on - strand at 2310.889 kb on + strand at 2310.928 kb on + strand at 2311.091 kb on + strand at 2311.204 kb on + strand at 2311.233 kb on + strand at 2311.245 kb on + strand at 2311.250 kb on - strand at 2311.342 kb on - strand at 2311.347 kb on + strand at 2311.347 kb on + strand at 2311.347 kb on + strand at 2311.399 kb on - strand at 2311.409 kb on - strand at 2311.421 kb on + strand at 2311.428 kb on - strand at 2311.428 kb on - strand at 2311.434 kb on - strand at 2311.435 kb on - strand at 2311.438 kb on - strand at 2311.438 kb on - strand at 2311.453 kb on - strand at 2311.453 kb on - strand at 2311.453 kb on - strand at 2311.457 kb on + strand at 2311.457 kb on + strand at 2311.458 kb on + strand at 2311.459 kb on + strand at 2311.459 kb on + strand at 2311.459 kb on + strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.467 kb on - strand at 2311.478 kb on - strand at 2311.552 kb on + strand at 2311.552 kb on + strand at 2311.586 kb on + strand at 2311.613 kb on + strand at 2311.613 kb on + strand at 2311.613 kb on - strand at 2311.687 kb on + strand at 2311.687 kb on + strand at 2311.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium glutamate 10 vol% remove 2,308,745 - yojL b2214 0.23 +0.3 2,308,784 - yojL b2214 0.27 +0.4 2,308,796 - yojL b2214 0.28 -0.2 2,308,916 + yojL b2214 0.39 +0.9 2,308,916 + yojL b2214 0.39 -0.0 2,308,964 - yojL b2214 0.44 +0.2 2,308,964 - yojL b2214 0.44 -0.7 2,309,070 - yojL b2214 0.54 -1.5 2,309,100 + yojL b2214 0.57 +0.3 2,309,123 - yojL b2214 0.59 +0.3 2,309,286 - yojL b2214 0.74 +0.7 2,309,286 - yojL b2214 0.74 +1.2 2,309,711 - +1.4 2,309,963 + ompC b2215 0.27 +0.4 2,309,981 + ompC b2215 0.28 +0.6 2,310,013 + ompC b2215 0.31 +1.8 2,310,044 + ompC b2215 0.34 -0.6 2,310,052 - ompC b2215 0.35 +1.3 2,310,052 - +0.8 2,310,081 - ompC b2215 0.37 +0.2 2,310,091 - ompC b2215 0.38 -0.5 2,310,155 + ompC b2215 0.44 +1.4 2,310,192 + ompC b2215 0.47 -1.4 2,310,277 + ompC b2215 0.55 -0.4 2,310,340 + ompC b2215 0.61 +0.0 2,310,362 - ompC b2215 0.63 +0.4 2,310,362 - ompC b2215 0.63 +0.9 2,310,362 - ompC b2215 0.63 +0.8 2,310,442 + ompC b2215 0.70 -0.7 2,310,442 + ompC b2215 0.70 +0.2 2,310,490 + ompC b2215 0.74 +1.0 2,310,531 + ompC b2215 0.78 +0.3 2,310,532 + ompC b2215 0.78 -1.2 2,310,538 - ompC b2215 0.79 +0.3 2,310,538 - ompC b2215 0.79 +0.3 2,310,562 - ompC b2215 0.81 +0.2 2,310,599 + ompC b2215 0.84 +1.2 2,310,601 + ompC b2215 0.85 +1.4 2,310,602 + ompC b2215 0.85 +0.2 2,310,602 + ompC b2215 0.85 +2.8 2,310,602 + ompC b2215 0.85 +0.1 2,310,602 + ompC b2215 0.85 -0.4 2,310,607 + ompC b2215 0.85 +3.8 2,310,607 + ompC b2215 0.85 -1.4 2,310,608 - ompC b2215 0.85 +0.4 2,310,610 - ompC b2215 0.85 +0.3 2,310,615 - ompC b2215 0.86 +0.5 2,310,629 - ompC b2215 0.87 +0.1 2,310,672 + +0.2 2,310,680 - +0.6 2,310,680 - +0.7 2,310,700 + +0.6 2,310,700 + +0.6 2,310,721 + -0.2 2,310,725 + -0.1 2,310,734 - +0.5 2,310,736 + -0.8 2,310,737 + +1.2 2,310,743 - -0.4 2,310,746 + +1.5 2,310,746 + +0.2 2,310,748 + -1.7 2,310,765 - +0.1 2,310,765 - -0.5 2,310,787 - +0.5 2,310,787 - +0.4 2,310,821 + -0.3 2,310,824 + +1.4 2,310,824 - -0.7 2,310,824 - +0.5 2,310,824 - +0.1 2,310,824 - -0.1 2,310,826 + +1.8 2,310,829 - -0.0 2,310,834 + -0.4 2,310,835 + +0.6 2,310,835 + -0.6 2,310,842 - +0.5 2,310,843 - +0.3 2,310,883 - -0.0 2,310,888 - +0.7 2,310,889 + +0.2 2,310,928 + -0.8 2,311,091 + +0.1 2,311,204 + +0.3 2,311,233 + +0.2 2,311,245 + +2.2 2,311,250 - -0.6 2,311,342 - +0.2 2,311,347 + +0.6 2,311,347 + +2.8 2,311,347 + -0.4 2,311,399 - +0.2 2,311,409 - +0.4 2,311,421 + +0.4 2,311,428 - -1.2 2,311,428 - +0.1 2,311,434 - -1.4 2,311,435 - +0.8 2,311,438 - +0.5 2,311,438 - +0.1 2,311,453 - +0.4 2,311,453 - -0.4 2,311,453 - -1.2 2,311,457 + +0.2 2,311,457 + +0.6 2,311,458 + -1.4 2,311,459 + +1.2 2,311,459 + +0.6 2,311,459 + +0.3 2,311,467 - -0.6 2,311,467 - +1.2 2,311,467 - +0.8 2,311,467 - +0.1 2,311,467 - -1.2 2,311,467 - +0.6 2,311,467 - +0.6 2,311,467 - -0.2 2,311,478 - -0.1 2,311,552 + -0.2 2,311,552 + -0.2 2,311,586 + +0.6 2,311,613 + +0.3 2,311,613 + -0.4 2,311,613 - -0.2 2,311,687 + +1.2 2,311,687 + -0.5 2,311,737 - -0.1
Or see this region's nucleotide sequence