Experiment: Cholinium glutamate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt narH and narJ overlap by 4 nucleotides narJ and narI overlap by 1 nucleotides narI and rttR are separated by 539 nucleotides rttR and tpr overlap by 150 nucleotides tpr and tyrV are separated by 67 nucleotides
b1225: narH - nitrate reductase 1, beta (Fe-S) subunit (NCBI), at 1,282,827 to 1,284,365
narH
b1226: narJ - molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1 (NCBI), at 1,284,362 to 1,285,072
narJ
b1227: narI - nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI), at 1,285,072 to 1,285,749
narI
b4425: rttR - regulatory sRNA (NCBI), at 1,286,289 to 1,286,459
rttR
b1229: tpr - predicted protamine-like protein (RefSeq), at 1,286,310 to 1,286,399
tpr
b1230: tyrV - tRNA-Tyr (NCBI), at 1,286,467 to 1,286,551
tyrV
Position (kb)
1285
1286 Strain fitness (log2 ratio)
-1
0
1
2 at 1284.099 kb on + strand, within narH at 1284.152 kb on + strand, within narH at 1284.436 kb on + strand, within narJ at 1284.436 kb on + strand, within narJ at 1284.480 kb on + strand, within narJ at 1284.554 kb on - strand, within narJ at 1284.634 kb on + strand, within narJ at 1284.728 kb on - strand, within narJ at 1284.809 kb on + strand, within narJ at 1284.809 kb on + strand, within narJ at 1285.100 kb on + strand at 1285.167 kb on + strand, within narI at 1285.352 kb on - strand, within narI at 1285.352 kb on - strand, within narI at 1285.476 kb on + strand, within narI at 1285.509 kb on - strand, within narI at 1285.518 kb on + strand, within narI at 1285.518 kb on + strand, within narI at 1285.526 kb on - strand, within narI at 1285.605 kb on - strand, within narI at 1285.670 kb on - strand, within narI at 1285.686 kb on + strand at 1285.694 kb on - strand at 1285.707 kb on + strand at 1286.125 kb on + strand at 1286.316 kb on - strand at 1286.316 kb on - strand at 1286.690 kb on - strand at 1286.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium glutamate 10 vol% remove 1,284,099 + narH b1225 0.83 +0.3 1,284,152 + narH b1225 0.86 -0.0 1,284,436 + narJ b1226 0.10 -0.2 1,284,436 + narJ b1226 0.10 -0.5 1,284,480 + narJ b1226 0.17 +0.7 1,284,554 - narJ b1226 0.27 +0.1 1,284,634 + narJ b1226 0.38 -0.3 1,284,728 - narJ b1226 0.51 +0.4 1,284,809 + narJ b1226 0.63 +1.2 1,284,809 + narJ b1226 0.63 +0.1 1,285,100 + -0.1 1,285,167 + narI b1227 0.14 +0.5 1,285,352 - narI b1227 0.41 +1.8 1,285,352 - narI b1227 0.41 -0.8 1,285,476 + narI b1227 0.60 +0.7 1,285,509 - narI b1227 0.64 +0.2 1,285,518 + narI b1227 0.66 -0.1 1,285,518 + narI b1227 0.66 +0.8 1,285,526 - narI b1227 0.67 +0.0 1,285,605 - narI b1227 0.79 +0.5 1,285,670 - narI b1227 0.88 +0.4 1,285,686 + +0.9 1,285,694 - +1.0 1,285,707 + -0.7 1,286,125 + +0.1 1,286,316 - -0.6 1,286,316 - -0.6 1,286,690 - +0.7 1,286,690 - +0.0
Or see this region's nucleotide sequence