Experiment: Cholinium glutamate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydB and ybgT are separated by 14 nucleotides ybgT and ybgE overlap by 1 nucleotides ybgE and ybgC are separated by 149 nucleotides ybgC and tolQ overlap by 4 nucleotides tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides
b0734: cydB - cytochrome d terminal oxidase, subunit II (NCBI), at 772,265 to 773,404
cydB
b4515: ybgT - hypothetical protein (NCBI), at 773,419 to 773,532
ybgT
b0735: ybgE - conserved inner membrane protein (NCBI), at 773,532 to 773,825
ybgE
b0736: ybgC - predicted acyl-CoA thioesterase (NCBI), at 773,975 to 774,379
ybgC
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
b0738: tolR - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 775,072 to 775,500
tolR
b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830
tolA
Position (kb) 774
775
776 Strain fitness (log2 ratio) -1
0
1
2
3 at 773.380 kb on + strand at 773.486 kb on + strand, within ybgT at 773.572 kb on + strand, within ybgE at 773.617 kb on - strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.857 kb on + strand at 773.857 kb on + strand at 773.900 kb on - strand at 773.900 kb on - strand at 773.968 kb on + strand at 773.974 kb on + strand at 773.974 kb on + strand at 774.007 kb on + strand at 774.007 kb on + strand at 774.103 kb on + strand, within ybgC at 774.103 kb on + strand, within ybgC at 774.256 kb on + strand, within ybgC at 774.265 kb on - strand, within ybgC at 774.299 kb on - strand, within ybgC at 774.336 kb on + strand, within ybgC at 774.390 kb on - strand at 774.561 kb on + strand, within tolQ at 774.571 kb on + strand, within tolQ at 774.649 kb on + strand, within tolQ at 774.668 kb on - strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.809 kb on - strand, within tolQ at 774.813 kb on - strand, within tolQ at 774.818 kb on - strand, within tolQ at 774.839 kb on + strand, within tolQ at 774.905 kb on + strand, within tolQ at 774.956 kb on + strand, within tolQ at 775.063 kb on + strand at 775.063 kb on + strand at 775.070 kb on - strand at 775.071 kb on - strand at 775.071 kb on - strand at 775.091 kb on - strand at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.146 kb on + strand, within tolR at 775.152 kb on - strand, within tolR at 775.194 kb on - strand, within tolR at 775.258 kb on + strand, within tolR at 775.265 kb on + strand, within tolR at 775.275 kb on + strand, within tolR at 775.280 kb on + strand, within tolR at 775.288 kb on - strand, within tolR at 775.288 kb on - strand, within tolR at 775.298 kb on - strand, within tolR at 775.333 kb on + strand, within tolR at 775.333 kb on + strand, within tolR at 775.357 kb on - strand, within tolR at 775.526 kb on - strand at 775.533 kb on + strand at 775.565 kb on + strand at 775.628 kb on + strand at 775.651 kb on + strand at 775.659 kb on - strand at 775.672 kb on + strand at 775.748 kb on + strand, within tolA at 775.753 kb on + strand, within tolA at 775.840 kb on + strand, within tolA at 775.930 kb on - strand, within tolA at 775.932 kb on + strand, within tolA at 775.947 kb on + strand, within tolA
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium glutamate 10 vol% remove 773,380 + +1.3 773,486 + ybgT b4515 0.59 +0.2 773,572 + ybgE b0735 0.14 +0.1 773,617 - ybgE b0735 0.29 +0.7 773,693 + ybgE b0735 0.55 +0.0 773,693 + ybgE b0735 0.55 +0.0 773,702 + ybgE b0735 0.58 +0.4 773,702 + ybgE b0735 0.58 +0.2 773,857 + +0.4 773,857 + +0.3 773,900 - -0.4 773,900 - +0.0 773,968 + -0.1 773,974 + +0.4 773,974 + -0.8 774,007 + +0.3 774,007 + -0.4 774,103 + ybgC b0736 0.32 +0.7 774,103 + ybgC b0736 0.32 -0.1 774,256 + ybgC b0736 0.69 -0.3 774,265 - ybgC b0736 0.72 +1.1 774,299 - ybgC b0736 0.80 -0.1 774,336 + ybgC b0736 0.89 -0.3 774,390 - +0.7 774,561 + tolQ b0737 0.27 +0.7 774,571 + tolQ b0737 0.28 +0.1 774,649 + tolQ b0737 0.39 +0.7 774,668 - tolQ b0737 0.42 +1.3 774,723 + tolQ b0737 0.50 +1.6 774,723 + tolQ b0737 0.50 +1.9 774,809 - tolQ b0737 0.62 +1.1 774,813 - tolQ b0737 0.63 -0.2 774,818 - tolQ b0737 0.64 -1.0 774,839 + tolQ b0737 0.67 -1.3 774,905 + tolQ b0737 0.76 +1.6 774,956 + tolQ b0737 0.84 +1.9 775,063 + -0.4 775,063 + -0.3 775,070 - +0.6 775,071 - +0.1 775,071 - +0.5 775,091 - -0.2 775,121 + tolR b0738 0.11 +0.1 775,121 + tolR b0738 0.11 -1.5 775,121 + tolR b0738 0.11 +0.3 775,146 + tolR b0738 0.17 +0.8 775,152 - tolR b0738 0.19 +2.1 775,194 - tolR b0738 0.28 +0.3 775,258 + tolR b0738 0.43 +0.3 775,265 + tolR b0738 0.45 +2.1 775,275 + tolR b0738 0.47 +0.3 775,280 + tolR b0738 0.48 +1.9 775,288 - tolR b0738 0.50 +0.9 775,288 - tolR b0738 0.50 +2.9 775,298 - tolR b0738 0.53 -0.5 775,333 + tolR b0738 0.61 +0.6 775,333 + tolR b0738 0.61 +1.1 775,357 - tolR b0738 0.66 -0.0 775,526 - -0.5 775,533 + -0.5 775,565 + -0.0 775,628 + -0.7 775,651 + +0.6 775,659 - +1.3 775,672 + +0.5 775,748 + tolA b0739 0.14 +1.7 775,753 + tolA b0739 0.15 +1.6 775,840 + tolA b0739 0.22 -0.0 775,930 - tolA b0739 0.29 +0.5 775,932 + tolA b0739 0.29 -0.2 775,947 + tolA b0739 0.30 +2.0
Or see this region's nucleotide sequence