Experiment: Cholinium glutamate 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcW and ylcI are separated by 164 nucleotides ylcI and nohB are separated by 388 nucleotides nohB and ylcF overlap by 26 nucleotides ylcF and tfaD overlap by 3 nucleotides tfaD and ybcY are separated by 54 nucleotides
b0559: ybcW - DLP12 prophage; predicted protein (NCBI), at 579,103 to 579,309
ybcW
b4589: ylcI - hypothetical protein (RefSeq), at 579,474 to 579,668
ylcI
b0560: nohB - DLP12 prophage; DNA packaging protein (NCBI), at 580,057 to 580,602
nohB
b4634: ylcF - no description, at 580,577 to 580,885
ylcF
b0561: tfaD - orf, hypothetical protein (VIMSS), at 580,883 to 581,320
tfaD
b0562: ybcY - orf, hypothetical protein (VIMSS), at 581,375 to 582,029
ybcY
Position (kb) 580
581 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 579.081 kb on - strand at 579.083 kb on - strand at 579.085 kb on + strand at 579.086 kb on + strand at 579.093 kb on - strand at 579.175 kb on + strand, within ybcW at 579.175 kb on + strand, within ybcW at 579.177 kb on + strand, within ybcW at 579.177 kb on + strand, within ybcW at 579.178 kb on + strand, within ybcW at 579.178 kb on + strand, within ybcW at 579.183 kb on + strand, within ybcW at 579.183 kb on + strand, within ybcW at 579.185 kb on - strand, within ybcW at 579.185 kb on - strand, within ybcW at 579.185 kb on - strand, within ybcW at 579.188 kb on + strand, within ybcW at 579.188 kb on + strand, within ybcW at 579.196 kb on - strand, within ybcW at 579.196 kb on - strand, within ybcW at 579.214 kb on - strand, within ybcW at 579.214 kb on - strand, within ybcW at 579.217 kb on - strand, within ybcW at 579.217 kb on - strand, within ybcW at 579.217 kb on - strand, within ybcW at 579.217 kb on - strand, within ybcW at 579.219 kb on - strand, within ybcW at 579.237 kb on - strand, within ybcW at 579.237 kb on - strand, within ybcW at 579.250 kb on - strand, within ybcW at 579.259 kb on + strand, within ybcW at 579.293 kb on - strand at 579.293 kb on - strand at 579.306 kb on - strand at 579.308 kb on + strand at 579.309 kb on - strand at 579.311 kb on + strand at 579.311 kb on + strand at 579.313 kb on + strand at 579.318 kb on + strand at 579.321 kb on - strand at 579.321 kb on - strand at 579.327 kb on - strand at 579.342 kb on + strand at 579.342 kb on + strand at 579.342 kb on + strand at 579.345 kb on - strand at 579.345 kb on - strand at 579.353 kb on + strand at 579.355 kb on - strand at 579.365 kb on + strand at 579.365 kb on + strand at 579.367 kb on - strand at 579.377 kb on - strand at 579.377 kb on - strand at 579.377 kb on - strand at 579.409 kb on - strand at 579.409 kb on - strand at 579.412 kb on - strand at 579.442 kb on - strand at 579.452 kb on - strand at 579.452 kb on - strand at 579.453 kb on + strand at 579.461 kb on + strand at 579.461 kb on + strand at 579.479 kb on + strand at 579.535 kb on - strand, within ylcI at 579.545 kb on - strand, within ylcI at 579.549 kb on - strand, within ylcI at 580.052 kb on + strand at 580.071 kb on - strand at 580.215 kb on - strand, within nohB at 580.215 kb on - strand, within nohB at 580.303 kb on - strand, within nohB at 580.605 kb on - strand at 580.642 kb on + strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.944 kb on - strand, within tfaD at 580.973 kb on + strand, within tfaD at 580.981 kb on - strand, within tfaD at 580.981 kb on - strand, within tfaD at 581.152 kb on + strand, within tfaD at 581.171 kb on + strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.252 kb on + strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.262 kb on + strand, within tfaD at 581.269 kb on - strand, within tfaD at 581.285 kb on + strand at 581.307 kb on + strand at 581.307 kb on + strand at 581.343 kb on + strand at 581.344 kb on + strand at 581.344 kb on + strand at 581.352 kb on - strand at 581.404 kb on + strand at 581.411 kb on + strand at 581.443 kb on + strand, within ybcY at 581.450 kb on + strand, within ybcY at 581.453 kb on - strand, within ybcY at 581.460 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.572 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.595 kb on + strand, within ybcY
Per-strain Table
Position Strand Gene LocusTag Fraction Cholinium glutamate 10 vol% remove 579,081 - -0.7 579,083 - -0.6 579,085 + +1.4 579,086 + +2.0 579,093 - +0.4 579,175 + ybcW b0559 0.35 -0.5 579,175 + ybcW b0559 0.35 -1.2 579,177 + ybcW b0559 0.36 -1.2 579,177 + ybcW b0559 0.36 -0.7 579,178 + ybcW b0559 0.36 +0.2 579,178 + ybcW b0559 0.36 -0.4 579,183 + ybcW b0559 0.39 -0.6 579,183 + ybcW b0559 0.39 +0.8 579,185 - ybcW b0559 0.40 +0.5 579,185 - ybcW b0559 0.40 +0.6 579,185 - ybcW b0559 0.40 -0.7 579,188 + ybcW b0559 0.41 -1.0 579,188 + ybcW b0559 0.41 +1.8 579,196 - ybcW b0559 0.45 -0.4 579,196 - ybcW b0559 0.45 -1.9 579,214 - ybcW b0559 0.54 +0.4 579,214 - ybcW b0559 0.54 -0.6 579,217 - ybcW b0559 0.55 -0.2 579,217 - ybcW b0559 0.55 +0.4 579,217 - ybcW b0559 0.55 -0.2 579,217 - ybcW b0559 0.55 +1.0 579,219 - ybcW b0559 0.56 -0.3 579,237 - ybcW b0559 0.65 -0.2 579,237 - ybcW b0559 0.65 -0.2 579,250 - ybcW b0559 0.71 -2.5 579,259 + ybcW b0559 0.75 -0.1 579,293 - -0.4 579,293 - -0.2 579,306 - +0.2 579,308 + +0.7 579,309 - -0.2 579,311 + +0.4 579,311 + -0.9 579,313 + -1.2 579,318 + -0.2 579,321 - +0.0 579,321 - +1.2 579,327 - +0.6 579,342 + +1.4 579,342 + -0.8 579,342 + -0.4 579,345 - +0.2 579,345 - -0.3 579,353 + +0.4 579,355 - -0.1 579,365 + -1.2 579,365 + +0.8 579,367 - +0.1 579,377 - +0.9 579,377 - +0.8 579,377 - +0.2 579,409 - +0.4 579,409 - -0.7 579,412 - +0.5 579,442 - -0.1 579,452 - +0.2 579,452 - +0.8 579,453 + -1.7 579,461 + -0.6 579,461 + +1.2 579,479 + -2.4 579,535 - ylcI b4589 0.31 +0.6 579,545 - ylcI b4589 0.36 +0.4 579,549 - ylcI b4589 0.38 +0.2 580,052 + +0.6 580,071 - +0.2 580,215 - nohB b0560 0.29 +0.6 580,215 - nohB b0560 0.29 +0.2 580,303 - nohB b0560 0.45 +0.6 580,605 - -1.3 580,642 + ylcF b4634 0.21 +0.1 580,668 - ylcF b4634 0.29 +3.4 580,668 - ylcF b4634 0.29 +0.4 580,733 + ylcF b4634 0.50 +1.7 580,733 + ylcF b4634 0.50 -0.1 580,733 + ylcF b4634 0.50 -0.2 580,944 - tfaD b0561 0.14 +0.2 580,973 + tfaD b0561 0.21 -0.3 580,981 - tfaD b0561 0.22 +0.1 580,981 - tfaD b0561 0.22 +0.5 581,152 + tfaD b0561 0.61 +1.4 581,171 + tfaD b0561 0.66 -0.4 581,192 - tfaD b0561 0.71 -0.4 581,192 - tfaD b0561 0.71 +2.4 581,252 + tfaD b0561 0.84 +0.8 581,260 - tfaD b0561 0.86 -0.6 581,260 - tfaD b0561 0.86 +3.0 581,262 + tfaD b0561 0.87 +0.1 581,269 - tfaD b0561 0.88 +0.1 581,285 + +0.0 581,307 + +0.5 581,307 + +0.1 581,343 + -0.9 581,344 + -0.0 581,344 + +0.1 581,352 - +1.4 581,404 + +1.3 581,411 + -0.3 581,443 + ybcY b0562 0.10 -0.4 581,450 + ybcY b0562 0.11 -0.6 581,453 - ybcY b0562 0.12 +0.2 581,460 + ybcY b0562 0.13 -0.8 581,524 + ybcY b0562 0.23 +0.8 581,524 + ybcY b0562 0.23 -2.3 581,572 + ybcY b0562 0.30 -0.6 581,574 + ybcY b0562 0.30 +1.1 581,574 + ybcY b0562 0.30 -0.0 581,576 + ybcY b0562 0.31 +2.6 581,576 + ybcY b0562 0.31 +0.0 581,584 - ybcY b0562 0.32 -0.2 581,584 - ybcY b0562 0.32 -0.3 581,595 + ybcY b0562 0.34 +1.7
Or see this region's nucleotide sequence