Strain Fitness in Escherichia coli BW25113 around b0032

Experiment: Cholinium glutamate 10 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Cholinium glutamate 10 vol%
29,587	+				-0.5
29,661	-				+1.2
29,661	-				+0.8
29,841	+	carA	b0032	0.17	+0.8
29,872	+	carA	b0032	0.19	+0.0
29,931	+	carA	b0032	0.24	+0.2
29,931	+	carA	b0032	0.24	+1.3
30,037	-	carA	b0032	0.34	+1.2
30,104	+	carA	b0032	0.39	+0.6
30,154	+	carA	b0032	0.44	+0.5
30,156	-	carA	b0032	0.44	+1.5
30,156	-	carA	b0032	0.44	+0.7
30,320	+	carA	b0032	0.58	+1.1
30,364	+	carA	b0032	0.62	-0.1
30,364	+	carA	b0032	0.62	+0.6
30,588	+	carA	b0032	0.82	+0.8
30,768	+				-0.1
30,770	+				+1.1
30,773	+				-0.0
30,786	-				+0.9
30,858	-				+1.1
30,868	+				+1.4
30,868	+				+1.3
30,889	-				+1.3
30,917	+				+1.4
30,917	+				+1.4
31,041	-				+1.4
31,099	+				+1.0
31,099	+				-0.5
31,099	+				+1.2
31,099	+				+0.3
31,101	+				+1.7
31,107	-				+1.2
31,144	-	carB	b0033	0.10	+1.1
31,151	+	carB	b0033	0.10	+1.2
31,157	-	carB	b0033	0.11	+0.9
31,157	-	carB	b0033	0.11	+1.0
31,161	+	carB	b0033	0.11	+1.3
31,188	-	carB	b0033	0.12	+0.8
31,190	+	carB	b0033	0.12	+1.2
31,242	-	carB	b0033	0.13	+1.1
31,259	-	carB	b0033	0.14	-0.2
31,327	+	carB	b0033	0.16	+0.5
31,327	+	carB	b0033	0.16	+1.3
31,341	+	carB	b0033	0.16	+1.2
31,359	+	carB	b0033	0.17	+1.0
31,359	+	carB	b0033	0.17	+1.5
31,360	+	carB	b0033	0.17	+1.0
31,425	-	carB	b0033	0.19	+1.2
31,466	+	carB	b0033	0.20	+0.1
31,643	+	carB	b0033	0.26	+1.2
31,726	-	carB	b0033	0.28	+1.8
31,748	+	carB	b0033	0.29	+1.3