Experiment: e-Amino-N-Caproic Acid 10 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgF and lysT are separated by 164 nucleotides lysT and valT are separated by 135 nucleotides valT and lysW are separated by 2 nucleotides lysW and valZ are separated by 149 nucleotides valZ and lysY are separated by 3 nucleotides lysY and lysZ are separated by 146 nucleotides lysZ and lysQ are separated by 132 nucleotides lysQ and nadA are separated by 432 nucleotides
b0742: ybgF - hypothetical protein (NCBI), at 778,821 to 779,612
ybgF
b0743: lysT - tRNA-Lys (NCBI), at 779,777 to 779,852
lysT
b0744: valT - tRNA-Val (NCBI), at 779,988 to 780,063
valT
b0745: lysW - tRNA-Lys (NCBI), at 780,066 to 780,141
lysW
b0746: valZ - tRNA-Val (NCBI), at 780,291 to 780,366
valZ
b0747: lysY - tRNA-Lys (NCBI), at 780,370 to 780,445
lysY
b0748: lysZ - tRNA-Lys (NCBI), at 780,592 to 780,667
lysZ
b0749: lysQ - tRNA-Lys (NCBI), at 780,800 to 780,875
lysQ
b0750: nadA - quinolinate synthetase (NCBI), at 781,308 to 782,351
nadA
Position (kb) 780
781 Strain fitness (log2 ratio) -1
0
1 at 779.342 kb on + strand, within ybgF at 779.342 kb on + strand, within ybgF at 779.392 kb on - strand, within ybgF at 779.428 kb on - strand, within ybgF at 779.510 kb on + strand, within ybgF at 779.544 kb on - strand at 779.544 kb on - strand at 779.565 kb on + strand at 779.610 kb on - strand at 779.619 kb on + strand at 780.222 kb on + strand at 780.222 kb on + strand at 780.232 kb on + strand at 780.232 kb on + strand at 780.242 kb on + strand at 780.245 kb on - strand at 780.274 kb on - strand at 780.357 kb on + strand, within valZ at 780.357 kb on + strand, within valZ at 780.536 kb on + strand at 780.536 kb on + strand at 780.583 kb on + strand at 780.703 kb on - strand at 780.705 kb on + strand at 780.708 kb on - strand at 780.729 kb on - strand at 781.165 kb on + strand at 781.257 kb on + strand at 781.257 kb on + strand at 781.262 kb on + strand at 781.262 kb on + strand at 781.319 kb on - strand at 781.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction e-Amino-N-Caproic Acid 10 vol% remove 779,342 + ybgF b0742 0.66 +0.2 779,342 + ybgF b0742 0.66 -0.1 779,392 - ybgF b0742 0.72 +1.2 779,428 - ybgF b0742 0.77 -1.2 779,510 + ybgF b0742 0.87 +0.5 779,544 - -0.7 779,544 - +0.3 779,565 + -0.1 779,610 - -0.0 779,619 + +0.6 780,222 + -0.8 780,222 + +1.3 780,232 + +1.0 780,232 + +0.0 780,242 + +0.3 780,245 - +1.7 780,274 - +0.0 780,357 + valZ b0746 0.87 +0.8 780,357 + valZ b0746 0.87 +0.2 780,536 + -0.8 780,536 + +0.0 780,583 + +0.2 780,703 - +1.0 780,705 + +0.0 780,708 - +0.0 780,729 - +0.1 781,165 + -0.2 781,257 + -0.6 781,257 + -0.3 781,262 + +0.5 781,262 + +0.1 781,319 - +0.2 781,319 - -0.5
Or see this region's nucleotide sequence