Strain Fitness in Escherichia coli BW25113 around b3029

Experiment: 1-Pentanol 0.5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygiZ and mdaB are separated by 318 nucleotidesmdaB and ygiN are separated by 30 nucleotidesygiN and parE are separated by 47 nucleotides b3027: ygiZ - conserved inner membrane protein (NCBI), at 3,169,901 to 3,170,233 ygiZ b3028: mdaB - NADPH quinone reductase (NCBI), at 3,170,552 to 3,171,133 mdaB b3029: ygiN - quinol monooxygenase (NCBI), at 3,171,164 to 3,171,478 ygiN b3030: parE - DNA topoisomerase IV subunit B (NCBI), at 3,171,526 to 3,173,418 parE Position (kb) 3171 3172Strain fitness (log2 ratio) -1 0 1 2 3at 3170.288 kb on - strandat 3170.356 kb on - strandat 3170.390 kb on - strandat 3170.395 kb on - strandat 3170.422 kb on - strandat 3170.422 kb on - strandat 3170.430 kb on - strandat 3170.438 kb on + strandat 3170.438 kb on + strandat 3170.446 kb on - strandat 3170.446 kb on - strandat 3170.521 kb on - strandat 3170.521 kb on - strandat 3170.521 kb on - strandat 3170.521 kb on - strandat 3170.596 kb on + strandat 3170.596 kb on + strandat 3170.601 kb on + strandat 3170.748 kb on - strand, within mdaBat 3170.748 kb on - strand, within mdaBat 3170.750 kb on + strand, within mdaBat 3170.761 kb on + strand, within mdaBat 3170.781 kb on - strand, within mdaBat 3170.828 kb on + strand, within mdaBat 3170.838 kb on - strand, within mdaBat 3170.894 kb on - strand, within mdaBat 3170.894 kb on - strand, within mdaBat 3170.978 kb on + strand, within mdaBat 3170.978 kb on + strand, within mdaBat 3170.986 kb on - strand, within mdaBat 3170.986 kb on - strand, within mdaBat 3170.988 kb on + strand, within mdaBat 3171.004 kb on + strand, within mdaBat 3171.098 kb on - strandat 3171.132 kb on + strandat 3171.132 kb on + strandat 3171.163 kb on - strandat 3171.209 kb on - strand, within ygiNat 3171.209 kb on - strand, within ygiNat 3171.223 kb on + strand, within ygiNat 3171.300 kb on + strand, within ygiNat 3171.473 kb on + strandat 3171.473 kb on + strandat 3171.486 kb on + strandat 3171.486 kb on + strandat 3171.494 kb on - strandat 3171.519 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-Pentanol 0.5 vol%
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3,170,288 - +1.4
3,170,356 - +0.8
3,170,390 - -0.1
3,170,395 - -0.9
3,170,422 - -0.6
3,170,422 - +0.4
3,170,430 - +0.3
3,170,438 + +0.4
3,170,438 + +1.4
3,170,446 - -0.3
3,170,446 - +0.1
3,170,521 - +1.4
3,170,521 - -0.1
3,170,521 - +0.3
3,170,521 - -0.5
3,170,596 + +0.2
3,170,596 + +0.1
3,170,601 + +0.0
3,170,748 - mdaB b3028 0.34 -0.3
3,170,748 - mdaB b3028 0.34 -0.6
3,170,750 + mdaB b3028 0.34 -0.1
3,170,761 + mdaB b3028 0.36 +0.3
3,170,781 - mdaB b3028 0.39 -0.3
3,170,828 + mdaB b3028 0.47 +0.1
3,170,838 - mdaB b3028 0.49 +0.1
3,170,894 - mdaB b3028 0.59 +3.2
3,170,894 - mdaB b3028 0.59 -0.2
3,170,978 + mdaB b3028 0.73 -0.3
3,170,978 + mdaB b3028 0.73 +0.4
3,170,986 - mdaB b3028 0.75 +0.1
3,170,986 - mdaB b3028 0.75 -0.4
3,170,988 + mdaB b3028 0.75 -0.5
3,171,004 + mdaB b3028 0.78 -0.4
3,171,098 - +0.1
3,171,132 + -0.4
3,171,132 + -1.3
3,171,163 - +0.8
3,171,209 - ygiN b3029 0.14 +0.8
3,171,209 - ygiN b3029 0.14 -0.9
3,171,223 + ygiN b3029 0.19 +1.0
3,171,300 + ygiN b3029 0.43 +0.3
3,171,473 + -0.1
3,171,473 + +0.4
3,171,486 + -0.0
3,171,486 + +0.1
3,171,494 - +0.1
3,171,519 - -0.1

Or see this region's nucleotide sequence