Experiment: 1-Pentanol 0.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliF and fliG overlap by 8 nucleotides fliG and fliH overlap by 8 nucleotides fliH and fliI overlap by 1 nucleotides
b1938: fliF - flagellar M-ring protein (NCBI), at 2,011,253 to 2,012,911
fliF
b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899
fliG
b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578
fliH
b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951
fliI
Position (kb)
2013
2014
2015 Strain fitness (log2 ratio)
-1
0
1 at 2012.922 kb on + strand at 2012.979 kb on + strand at 2012.979 kb on + strand at 2013.108 kb on + strand, within fliG at 2013.108 kb on + strand, within fliG at 2013.149 kb on - strand, within fliG at 2013.444 kb on + strand, within fliG at 2014.036 kb on + strand, within fliH at 2014.099 kb on - strand, within fliH at 2014.120 kb on + strand, within fliH at 2014.214 kb on + strand, within fliH at 2014.240 kb on - strand, within fliH at 2014.246 kb on + strand, within fliH at 2014.318 kb on - strand, within fliH at 2014.336 kb on + strand, within fliH at 2014.404 kb on - strand, within fliH at 2014.562 kb on + strand at 2014.562 kb on + strand at 2014.829 kb on + strand, within fliI at 2014.829 kb on + strand, within fliI at 2014.917 kb on - strand, within fliI at 2014.920 kb on + strand, within fliI at 2014.920 kb on + strand, within fliI at 2015.057 kb on - strand, within fliI at 2015.057 kb on - strand at 2015.259 kb on - strand, within fliI at 2015.261 kb on - strand, within fliI at 2015.261 kb on - strand, within fliI at 2015.499 kb on - strand, within fliI at 2015.549 kb on + strand, within fliI at 2015.549 kb on + strand, within fliI at 2015.560 kb on + strand, within fliI
Per-strain Table
Position Strand Gene LocusTag Fraction 1-Pentanol 0.5 vol% remove 2,012,922 + +0.4 2,012,979 + +0.1 2,012,979 + -0.3 2,013,108 + fliG b1939 0.20 -0.0 2,013,108 + fliG b1939 0.20 +0.5 2,013,149 - fliG b1939 0.25 -0.4 2,013,444 + fliG b1939 0.54 -0.3 2,014,036 + fliH b1940 0.21 +0.3 2,014,099 - fliH b1940 0.30 -0.1 2,014,120 + fliH b1940 0.33 +0.2 2,014,214 + fliH b1940 0.47 +0.4 2,014,240 - fliH b1940 0.51 -0.2 2,014,246 + fliH b1940 0.52 +1.1 2,014,318 - fliH b1940 0.62 +1.1 2,014,336 + fliH b1940 0.65 +0.6 2,014,404 - fliH b1940 0.75 +0.6 2,014,562 + -1.2 2,014,562 + -0.3 2,014,829 + fliI b1941 0.18 -0.3 2,014,829 + fliI b1941 0.18 -0.6 2,014,917 - fliI b1941 0.25 -0.4 2,014,920 + fliI b1941 0.25 +0.0 2,014,920 + fliI b1941 0.25 -0.1 2,015,057 - fliI b1941 0.35 +1.4 2,015,057 - -0.3 2,015,259 - fliI b1941 0.50 -0.2 2,015,261 - fliI b1941 0.50 +1.7 2,015,261 - fliI b1941 0.50 -0.2 2,015,499 - fliI b1941 0.67 +0.5 2,015,549 + fliI b1941 0.71 -0.5 2,015,549 + fliI b1941 0.71 -0.7 2,015,560 + fliI b1941 0.71 +0.3
Or see this region's nucleotide sequence