Experiment: Caprolactam 7 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaJ and yeaK are separated by 42 nucleotides yeaK and b1788 are separated by 0 nucleotides b1788 and yeaL are separated by 169 nucleotides yeaL and yeaM overlap by 44 nucleotides yeaM and yeaN are separated by 96 nucleotides
b1786: yeaJ - orf, hypothetical protein (VIMSS), at 1,870,065 to 1,871,555
yeaJ
b1787: yeaK - hypothetical protein (NCBI), at 1,871,598 to 1,872,101
yeaK
b1788: b1788 - orf, hypothetical protein (VIMSS), at 1,872,102 to 1,872,206
b1788
b1789: yeaL - conserved inner membrane protein (NCBI), at 1,872,376 to 1,872,822
yeaL
b1790: yeaM - predicted DNA-binding transcriptional regulator (NCBI), at 1,872,779 to 1,873,600
yeaM
b1791: yeaN - predicted transporter (NCBI), at 1,873,697 to 1,874,878
yeaN
Position (kb)
1872
1873 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1871.390 kb on + strand, within yeaJ at 1871.396 kb on - strand, within yeaJ at 1871.414 kb on + strand at 1871.414 kb on + strand at 1871.436 kb on + strand at 1871.483 kb on + strand at 1871.495 kb on + strand at 1871.495 kb on + strand at 1871.502 kb on - strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.551 kb on + strand at 1871.551 kb on + strand at 1871.581 kb on + strand at 1871.581 kb on + strand at 1871.734 kb on + strand, within yeaK at 1871.741 kb on - strand, within yeaK at 1871.746 kb on - strand, within yeaK at 1871.748 kb on + strand, within yeaK at 1871.748 kb on + strand, within yeaK at 1871.754 kb on - strand, within yeaK at 1871.754 kb on - strand, within yeaK at 1871.759 kb on + strand, within yeaK at 1871.790 kb on - strand, within yeaK at 1871.804 kb on - strand, within yeaK at 1871.823 kb on - strand, within yeaK at 1871.909 kb on - strand, within yeaK at 1871.909 kb on - strand, within yeaK at 1871.978 kb on - strand, within yeaK at 1872.285 kb on - strand at 1872.399 kb on - strand at 1872.443 kb on - strand, within yeaL at 1872.584 kb on + strand, within yeaL at 1872.606 kb on - strand, within yeaL at 1872.672 kb on - strand, within yeaL at 1872.672 kb on - strand, within yeaL at 1872.680 kb on + strand, within yeaL at 1872.680 kb on + strand, within yeaL at 1873.148 kb on + strand, within yeaM at 1873.370 kb on + strand, within yeaM at 1873.391 kb on - strand, within yeaM at 1873.472 kb on + strand, within yeaM at 1873.472 kb on + strand, within yeaM at 1873.510 kb on + strand, within yeaM at 1873.559 kb on - strand at 1873.744 kb on - strand at 1873.791 kb on + strand at 1873.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Caprolactam 7 vol% remove 1,871,390 + yeaJ b1786 0.89 +0.9 1,871,396 - yeaJ b1786 0.89 +0.1 1,871,414 + +0.5 1,871,414 + -0.6 1,871,436 + +0.1 1,871,483 + -0.4 1,871,495 + +0.1 1,871,495 + +0.7 1,871,502 - -0.4 1,871,534 + -1.6 1,871,534 + +0.1 1,871,534 + +0.9 1,871,534 + -0.2 1,871,551 + +1.3 1,871,551 + -0.4 1,871,581 + -0.7 1,871,581 + +0.1 1,871,734 + yeaK b1787 0.27 +0.3 1,871,741 - yeaK b1787 0.28 +0.1 1,871,746 - yeaK b1787 0.29 +0.8 1,871,748 + yeaK b1787 0.30 -0.2 1,871,748 + yeaK b1787 0.30 +0.6 1,871,754 - yeaK b1787 0.31 +0.3 1,871,754 - yeaK b1787 0.31 -2.3 1,871,759 + yeaK b1787 0.32 -0.1 1,871,790 - yeaK b1787 0.38 -0.5 1,871,804 - yeaK b1787 0.41 +1.9 1,871,823 - yeaK b1787 0.45 -0.2 1,871,909 - yeaK b1787 0.62 -0.4 1,871,909 - yeaK b1787 0.62 -0.9 1,871,978 - yeaK b1787 0.75 +0.2 1,872,285 - -0.1 1,872,399 - +0.2 1,872,443 - yeaL b1789 0.15 -0.2 1,872,584 + yeaL b1789 0.47 +0.3 1,872,606 - yeaL b1789 0.51 -0.2 1,872,672 - yeaL b1789 0.66 +0.2 1,872,672 - yeaL b1789 0.66 +1.0 1,872,680 + yeaL b1789 0.68 +0.8 1,872,680 + yeaL b1789 0.68 +0.7 1,873,148 + yeaM b1790 0.45 -0.5 1,873,370 + yeaM b1790 0.72 +0.2 1,873,391 - yeaM b1790 0.74 +0.3 1,873,472 + yeaM b1790 0.84 -0.1 1,873,472 + yeaM b1790 0.84 +0.4 1,873,510 + yeaM b1790 0.89 -0.1 1,873,559 - -0.8 1,873,744 - +0.0 1,873,791 + +0.3 1,873,799 - +0.8
Or see this region's nucleotide sequence