Experiment: Caprolactam 7 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcW and yncL are separated by 96 nucleotides yncL and b1445 are separated by 194 nucleotides b1445 and ydcY are separated by 85 nucleotides ydcY and ydcZ are separated by 0 nucleotides ydcZ and yncA overlap by 4 nucleotides
b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026
ydcW
b4598: yncL - hypothetical protein (RefSeq), at 1,515,123 to 1,515,218
yncL
b1445: b1445 - orf, hypothetical protein (VIMSS), at 1,515,413 to 1,515,586
b1445
b1446: ydcY - hypothetical protein (NCBI), at 1,515,672 to 1,515,905
ydcY
b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355
ydcZ
b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870
yncA
Position (kb)
1515
1516 Strain fitness (log2 ratio)
-1
0
1 at 1514.706 kb on + strand, within ydcW at 1514.920 kb on + strand at 1515.090 kb on + strand at 1515.199 kb on - strand, within yncL at 1515.247 kb on + strand at 1515.249 kb on + strand at 1515.252 kb on - strand at 1515.257 kb on - strand at 1515.257 kb on - strand at 1515.259 kb on + strand at 1515.608 kb on + strand at 1515.670 kb on - strand at 1515.791 kb on + strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.892 kb on + strand at 1515.892 kb on + strand at 1516.613 kb on - strand, within yncA at 1516.789 kb on - strand, within yncA at 1516.831 kb on + strand at 1516.870 kb on + strand at 1516.878 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Caprolactam 7 vol% remove 1,514,706 + ydcW b1444 0.77 -0.0 1,514,920 + +0.2 1,515,090 + +1.5 1,515,199 - yncL b4598 0.79 +0.3 1,515,247 + +1.3 1,515,249 + -0.4 1,515,252 - +0.0 1,515,257 - -0.1 1,515,257 - -0.9 1,515,259 + +0.2 1,515,608 + +0.1 1,515,670 - -0.4 1,515,791 + ydcY b1446 0.51 +1.2 1,515,839 - ydcY b1446 0.71 +0.3 1,515,839 - ydcY b1446 0.71 +0.1 1,515,892 + +0.3 1,515,892 + -0.4 1,516,613 - yncA b1448 0.50 -0.1 1,516,789 - yncA b1448 0.84 +0.1 1,516,831 + -0.0 1,516,870 + -0.0 1,516,878 - +0.1
Or see this region's nucleotide sequence