Experiment: Caprolactam 7 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frmA and yaiN are separated by 34 nucleotides yaiN and yaiO are separated by 187 nucleotides yaiO and yaiX are separated by 1 nucleotides
b0356: frmA - alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI), at 377,686 to 378,795
frmA
b0357: yaiN - putative alpha helix chain (VIMSS), at 378,830 to 379,105
yaiN
b0358: yaiO - hypothetical protein (NCBI), at 379,293 to 380,066
yaiO
b4579: yaiX - no description, at 380,068 to 380,483
yaiX
Position (kb) 378
379
380 Strain fitness (log2 ratio) -2
-1
0
1
2 at 377.859 kb on - strand, within frmA at 378.034 kb on - strand, within frmA at 378.051 kb on + strand, within frmA at 378.051 kb on + strand, within frmA at 378.135 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.283 kb on - strand, within frmA at 378.383 kb on - strand, within frmA at 378.383 kb on - strand, within frmA at 378.449 kb on + strand, within frmA at 378.479 kb on - strand, within frmA at 378.479 kb on - strand, within frmA at 378.484 kb on - strand, within frmA at 378.523 kb on - strand, within frmA at 378.626 kb on + strand, within frmA at 378.963 kb on - strand, within yaiN at 378.963 kb on - strand, within yaiN at 379.025 kb on + strand, within yaiN at 379.409 kb on - strand, within yaiO at 379.409 kb on - strand, within yaiO at 379.439 kb on - strand, within yaiO at 379.480 kb on - strand, within yaiO at 379.481 kb on - strand, within yaiO at 379.573 kb on + strand, within yaiO at 379.573 kb on + strand, within yaiO at 379.580 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.618 kb on + strand, within yaiO at 379.618 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.625 kb on + strand, within yaiO at 379.625 kb on + strand, within yaiO at 379.628 kb on + strand, within yaiO at 379.628 kb on + strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.770 kb on - strand, within yaiO at 379.779 kb on + strand, within yaiO at 379.779 kb on + strand, within yaiO at 379.787 kb on - strand, within yaiO at 379.787 kb on - strand, within yaiO at 379.788 kb on + strand, within yaiO at 379.798 kb on - strand, within yaiO at 379.798 kb on - strand, within yaiO at 379.814 kb on + strand, within yaiO at 379.825 kb on + strand, within yaiO at 379.831 kb on + strand, within yaiO at 379.831 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.837 kb on - strand, within yaiO at 379.848 kb on + strand, within yaiO at 379.848 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.936 kb on - strand, within yaiO at 379.988 kb on - strand, within yaiO at 379.993 kb on + strand at 379.993 kb on + strand at 379.995 kb on + strand at 380.009 kb on + strand at 380.088 kb on + strand at 380.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Caprolactam 7 vol% remove 377,859 - frmA b0356 0.16 +0.0 378,034 - frmA b0356 0.31 -0.8 378,051 + frmA b0356 0.33 +1.0 378,051 + frmA b0356 0.33 +0.7 378,135 + frmA b0356 0.40 -0.1 378,256 + frmA b0356 0.51 -0.4 378,256 + frmA b0356 0.51 -0.1 378,256 + frmA b0356 0.51 -0.2 378,283 - frmA b0356 0.54 +0.0 378,383 - frmA b0356 0.63 -0.9 378,383 - frmA b0356 0.63 +1.0 378,449 + frmA b0356 0.69 -0.1 378,479 - frmA b0356 0.71 -0.0 378,479 - frmA b0356 0.71 -0.4 378,484 - frmA b0356 0.72 -0.4 378,523 - frmA b0356 0.75 -0.3 378,626 + frmA b0356 0.85 +0.7 378,963 - yaiN b0357 0.48 +0.7 378,963 - yaiN b0357 0.48 +0.3 379,025 + yaiN b0357 0.71 +0.3 379,409 - yaiO b0358 0.15 -0.0 379,409 - yaiO b0358 0.15 +0.2 379,439 - yaiO b0358 0.19 +0.2 379,480 - yaiO b0358 0.24 +1.3 379,481 - yaiO b0358 0.24 +0.3 379,573 + yaiO b0358 0.36 -0.9 379,573 + yaiO b0358 0.36 +0.1 379,580 + yaiO b0358 0.37 +0.1 379,616 + yaiO b0358 0.42 -0.5 379,616 + yaiO b0358 0.42 -1.6 379,616 + yaiO b0358 0.42 -0.1 379,616 + yaiO b0358 0.42 -0.2 379,616 + yaiO b0358 0.42 -0.4 379,616 + yaiO b0358 0.42 +0.7 379,616 + yaiO b0358 0.42 -0.8 379,616 + yaiO b0358 0.42 +0.6 379,618 + yaiO b0358 0.42 +0.2 379,618 + yaiO b0358 0.42 -0.6 379,621 + yaiO b0358 0.42 -0.2 379,621 + yaiO b0358 0.42 -0.4 379,621 + yaiO b0358 0.42 -0.2 379,621 + yaiO b0358 0.42 +0.3 379,621 + yaiO b0358 0.42 +0.2 379,621 + yaiO b0358 0.42 +1.5 379,625 + yaiO b0358 0.43 -0.7 379,625 + yaiO b0358 0.43 +0.2 379,628 + yaiO b0358 0.43 +0.1 379,628 + yaiO b0358 0.43 -0.7 379,629 - yaiO b0358 0.43 +0.3 379,629 - yaiO b0358 0.43 +0.5 379,629 - yaiO b0358 0.43 +0.5 379,629 - yaiO b0358 0.43 +1.2 379,629 - yaiO b0358 0.43 -0.2 379,629 - yaiO b0358 0.43 +0.1 379,770 - yaiO b0358 0.62 -1.8 379,779 + yaiO b0358 0.63 +0.7 379,779 + yaiO b0358 0.63 +0.2 379,787 - yaiO b0358 0.64 +1.5 379,787 - yaiO b0358 0.64 +1.5 379,788 + yaiO b0358 0.64 +0.1 379,798 - yaiO b0358 0.65 -0.7 379,798 - yaiO b0358 0.65 -1.2 379,814 + yaiO b0358 0.67 +0.2 379,825 + yaiO b0358 0.69 +0.1 379,831 + yaiO b0358 0.70 +0.2 379,831 - yaiO b0358 0.70 -0.0 379,833 - yaiO b0358 0.70 +0.0 379,833 - yaiO b0358 0.70 +0.4 379,833 - yaiO b0358 0.70 +1.0 379,833 - yaiO b0358 0.70 +0.8 379,833 - yaiO b0358 0.70 -2.3 379,837 - yaiO b0358 0.70 +0.5 379,848 + yaiO b0358 0.72 -0.1 379,848 + yaiO b0358 0.72 -0.8 379,928 + yaiO b0358 0.82 +0.4 379,928 + yaiO b0358 0.82 +0.0 379,928 + yaiO b0358 0.82 -0.2 379,928 + yaiO b0358 0.82 +0.2 379,936 - yaiO b0358 0.83 -1.1 379,988 - yaiO b0358 0.90 +0.3 379,993 + +0.9 379,993 + +2.1 379,995 + -0.1 380,009 + -0.1 380,088 + -0.1 380,088 + +1.5
Or see this region's nucleotide sequence