Experiment: Isobutyl alcohol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafT and ykfM are separated by 136 nucleotides ykfM and yafU are separated by 9 nucleotides yafU and yafF are separated by 3 nucleotides yafF and yafV are separated by 40 nucleotides yafV and ivy are separated by 153 nucleotides
b0217: yafT - predicted aminopeptidase (NCBI), at 237,335 to 238,120
yafT
b4586: ykfM - hypothetical protein, no homologs (RefSeq), at 238,257 to 238,736
ykfM
b0218: yafU - predicted inner membrane protein (RefSeq), at 238,746 to 239,102
yafU
b4503: yafF - no description, at 239,106 to 239,378
yafF
b0219: yafV - predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI), at 239,419 to 240,189
yafV
b0220: ivy - inhibitor of vertebrate C-lysozyme (NCBI), at 240,343 to 240,816
ivy
Position (kb) 239
240 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 238.734 kb on - strand at 238.901 kb on + strand, within yafU at 238.910 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.036 kb on - strand, within yafU at 239.036 kb on - strand, within yafU at 239.163 kb on + strand, within yafF at 239.166 kb on + strand, within yafF at 239.392 kb on - strand at 239.412 kb on + strand at 239.414 kb on - strand at 239.417 kb on + strand at 239.426 kb on + strand at 239.436 kb on - strand at 239.436 kb on - strand at 239.850 kb on - strand, within yafV at 239.850 kb on - strand, within yafV at 239.907 kb on + strand, within yafV at 240.066 kb on + strand, within yafV at 240.072 kb on + strand, within yafV at 240.098 kb on - strand, within yafV at 240.145 kb on + strand at 240.148 kb on + strand at 240.175 kb on + strand at 240.197 kb on - strand at 240.197 kb on - strand at 240.199 kb on - strand at 240.199 kb on - strand at 240.224 kb on + strand at 240.261 kb on - strand at 240.325 kb on + strand at 240.325 kb on + strand at 240.333 kb on + strand at 240.350 kb on + strand at 240.350 kb on + strand at 240.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol% remove 238,734 - +1.6 238,901 + yafU b0218 0.43 +1.6 238,910 - yafU b0218 0.46 -0.4 238,915 - yafU b0218 0.47 -0.6 238,915 - yafU b0218 0.47 +0.4 238,915 - yafU b0218 0.47 -0.4 239,013 + yafU b0218 0.75 -0.0 239,013 + yafU b0218 0.75 +0.6 239,013 + yafU b0218 0.75 +1.6 239,036 - yafU b0218 0.81 -0.4 239,036 - yafU b0218 0.81 +0.3 239,163 + yafF b4503 0.21 -0.4 239,166 + yafF b4503 0.22 +1.4 239,392 - +0.3 239,412 + -2.3 239,414 - +1.4 239,417 + -0.1 239,426 + +0.1 239,436 - -0.3 239,436 - +0.9 239,850 - yafV b0219 0.56 +1.0 239,850 - yafV b0219 0.56 +0.7 239,907 + yafV b0219 0.63 +0.1 240,066 + yafV b0219 0.84 +0.2 240,072 + yafV b0219 0.85 -1.2 240,098 - yafV b0219 0.88 -0.7 240,145 + -1.0 240,148 + +0.1 240,175 + +1.0 240,197 - -0.6 240,197 - -0.2 240,199 - -0.2 240,199 - +3.1 240,224 + -0.1 240,261 - -1.1 240,325 + -0.2 240,325 + -0.6 240,333 + -0.0 240,350 + -1.2 240,350 + -0.2 240,358 - -1.2
Or see this region's nucleotide sequence