Strain Fitness in Escherichia coli BW25113 around b4280

Experiment: Isobutyl alcohol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Isobutyl alcohol 1.25 vol%
4,502,363	+	yjhB	b4279	0.23	+1.7
4,502,794	+	yjhB	b4279	0.59	+1.7
4,503,626	+	yjhC	b4280	0.28	+1.7
4,503,638	+	yjhC	b4280	0.29	+1.7
4,503,647	+	yjhC	b4280	0.30	+0.7
4,503,648	+	yjhC	b4280	0.30	+0.7
4,503,648	+	yjhC	b4280	0.30	+0.7
4,503,814	+	yjhC	b4280	0.45	+0.7
4,503,826	+	yjhC	b4280	0.46	+1.7
4,503,830	+	yjhC	b4280	0.46	+0.7
4,503,900	+	yjhC	b4280	0.53	+0.7
4,503,911	+	yjhC	b4280	0.54	+0.6
4,503,911	+	yjhC	b4280	0.54	+0.7
4,503,985	+	yjhC	b4280	0.60	+1.7
4,503,985	+	yjhC	b4280	0.60	+0.5
4,503,986	+	yjhC	b4280	0.60	+1.7
4,503,990	+	yjhC	b4280	0.61	+0.1
4,503,991	+	yjhC	b4280	0.61	+0.1
4,503,991	+	yjhC	b4280	0.61	+0.1
4,503,999	-	yjhC	b4280	0.62	+1.7
4,504,003	+	yjhC	b4280	0.62	-1.6
4,504,018	+	yjhC	b4280	0.63	+0.6
4,504,084	-	yjhC	b4280	0.69	+1.7
4,504,085	+	yjhC	b4280	0.69	+2.7
4,504,086	+	yjhC	b4280	0.69	-0.2
4,504,086	+	yjhC	b4280	0.69	+1.4
4,504,091	+	yjhC	b4280	0.70	+0.7
4,504,138	+	yjhC	b4280	0.74	+0.8
4,504,159	+	yjhC	b4280	0.76	+0.1
4,504,159	+	yjhC	b4280	0.76	+0.4
4,504,174	+	yjhC	b4280	0.77	+0.4
4,504,184	+	yjhC	b4280	0.78	+0.1
4,504,184	+	yjhC	b4280	0.78	+0.1
4,504,187	+	yjhC	b4280	0.78	+0.3
4,504,187	+	yjhC	b4280	0.78	+0.0
4,504,191	+	yjhC	b4280	0.79	+0.7
4,504,191	+	yjhC	b4280	0.79	-0.2
4,504,191	+	yjhC	b4280	0.79	+1.1
4,504,191	+	yjhC	b4280	0.79	-0.0
4,504,191	+	yjhC	b4280	0.79	+1.7
4,504,221	+	yjhC	b4280	0.81	+0.5
4,504,268	+	yjhC	b4280	0.86	+0.4
4,504,277	-	yjhC	b4280	0.86	+0.7
4,504,316	+	yjhC	b4280	0.90	+0.3
4,504,318	+				-0.5
4,504,318	+				+1.7
4,504,319	+				+0.1
4,504,319	+				-0.6
4,504,319	-				+0.7
4,504,322	-				-0.2
4,504,327	-				+3.3
4,504,334	-				+1.7
4,504,334	-				+1.7
4,504,336	+				-0.2
4,504,346	+				-0.4
4,504,346	+				-0.1
4,504,347	+				-0.2
4,504,348	+				-0.3
4,504,348	+				-0.1
4,504,361	+				+0.0
4,504,362	+				+0.5
4,504,362	+				-0.2
4,504,363	+				-0.3
4,504,370	+				+0.4
4,504,370	+				-1.0
4,504,396	+				+0.1
4,504,396	+				+0.2
4,504,403	+				-0.1
4,504,404	+				+0.1
4,504,413	+				-0.2
4,504,572	-	ythA	b4655	0.80	-0.1
4,504,576	-	ythA	b4655	0.83	-0.6
4,504,576	-	ythA	b4655	0.83	+0.9
4,504,613	-				-0.7
4,504,613	-				-0.9
4,504,613	-				-0.3
4,504,613	-				+0.2
4,504,613	-				+0.4
4,504,626	-				-0.4
4,504,626	-				+0.5
4,504,628	-				+0.0
4,504,629	-				+0.4
4,504,631	+				+1.7
4,504,647	-				-0.1
4,504,680	-	yjhD	b4281	0.13	+0.1
4,504,682	-	yjhD	b4281	0.14	-0.5
4,504,682	-	yjhD	b4281	0.14	+0.1
4,504,682	-	yjhD	b4281	0.14	+0.2
4,504,683	+	yjhD	b4281	0.15	+0.7
4,504,683	-	yjhD	b4281	0.15	-0.4
4,504,691	-	yjhD	b4281	0.18	-0.1
4,504,694	-	yjhD	b4281	0.19	+0.2
4,504,694	-	yjhD	b4281	0.19	-0.0
4,504,706	-	yjhD	b4281	0.25	+1.0
4,504,706	-	yjhD	b4281	0.25	-1.0
4,504,714	+	yjhD	b4281	0.28	+1.7
4,504,714	+	yjhD	b4281	0.28	+1.7
4,504,722	-	yjhD	b4281	0.32	+0.7
4,504,722	-	yjhD	b4281	0.32	-0.4
4,504,736	-	yjhD	b4281	0.38	+0.1
4,504,739	-	yjhD	b4281	0.39	+0.3
4,504,741	-	yjhD	b4281	0.40	+0.3
4,504,749	+	yjhD	b4281	0.43	+1.7
4,504,751	-	yjhD	b4281	0.44	-0.1
4,504,751	-	yjhD	b4281	0.44	-0.3
4,504,758	-	yjhD	b4281	0.47	+0.4
4,504,758	-	yjhD	b4281	0.47	+0.4
4,504,765	-	yjhD	b4281	0.50	-1.6
4,504,793	-	yjhD	b4281	0.62	+0.1
4,504,823	+	yjhD	b4281	0.75	-1.1
4,504,823	+	yjhD	b4281	0.75	+0.7
4,504,823	+	yjhD	b4281	0.75	+0.1
4,504,831	-	yjhD	b4281	0.79	+0.1
4,504,831	-	yjhD	b4281	0.79	+0.8
4,504,831	-	yjhD	b4281	0.79	-1.4
4,504,831	-	yjhD	b4281	0.79	+0.4
4,504,840	-	yjhD	b4281	0.83	-0.4
4,504,840	-	yjhD	b4281	0.83	-0.8
4,504,841	-	yjhD	b4281	0.83	-0.5
4,504,841	-	yjhD	b4281	0.83	-0.9
4,504,841	-	yjhD	b4281	0.83	-0.7
4,504,841	-	yjhD	b4281	0.83	+0.1
4,504,841	-	yjhD	b4281	0.83	-0.3
4,504,842	+	yjhD	b4281	0.84	+0.7
4,504,842	+	yjhD	b4281	0.84	+1.7
4,504,842	+	yjhD	b4281	0.84	+0.4
4,504,850	-	yjhD	b4281	0.87	+0.0
4,504,850	-	yjhD	b4281	0.87	+0.4
4,504,852	+	yjhD	b4281	0.88	+1.0
4,504,852	+	yjhD	b4281	0.88	-1.0
4,504,852	+	yjhD	b4281	0.88	+0.3
4,504,852	-	yjhD	b4281	0.88	-0.3
4,504,854	+	yjhD	b4281	0.89	+1.1
4,504,859	+				-1.5
4,504,859	+				+1.0
4,504,859	-				+0.1
4,504,861	-				-0.5
4,504,864	-				+0.2
4,504,864	-				+0.1
4,504,864	-				-0.0
4,504,866	+				+1.1
4,504,874	+				-0.5
4,504,874	-				-0.2
4,504,874	-				+0.3
4,504,882	-				+0.1
4,504,882	-				-0.1
4,504,882	-				-0.8
4,504,882	-				-0.1
4,504,882	-				+0.0
4,504,889	-				-1.3
4,504,906	-				-0.0
4,504,906	-				-0.2
4,504,906	-				-0.6
4,504,907	-				-0.5
4,504,907	-				-0.7
4,504,911	-	yjhE	b4282	0.11	+0.7
4,504,912	-	yjhE	b4282	0.11	-0.0
4,504,913	+	yjhE	b4282	0.12	-1.1
4,504,913	+	yjhE	b4282	0.12	-1.0
4,504,913	+	yjhE	b4282	0.12	+1.1
4,504,914	+	yjhE	b4282	0.12	+0.4
4,504,921	-	yjhE	b4282	0.15	-1.2
4,504,923	+	yjhE	b4282	0.16	+0.0
4,504,923	+	yjhE	b4282	0.16	-0.6
4,504,923	+	yjhE	b4282	0.16	-0.2
4,504,933	+	yjhE	b4282	0.20	+0.3
4,504,933	+	yjhE	b4282	0.20	+0.2
4,504,973	-	yjhE	b4282	0.36	-0.6
4,505,015	-	yjhE	b4282	0.53	-0.5
4,505,058	+	yjhE	b4282	0.70	+0.5
4,505,058	+	yjhE	b4282	0.70	-0.3
4,505,078	+	yjhE	b4282	0.78	-0.6
4,505,078	+	yjhE	b4282	0.78	-0.5
4,505,097	-	yjhE	b4282	0.86	+0.2
4,505,097	-	yjhE	b4282	0.86	-0.4
4,505,121	-				+0.1
4,505,123	-				+0.4
4,505,124	-				-0.6
4,505,126	-				-0.0
4,505,126	-				+0.6
4,505,144	+				+0.1
4,505,147	-				-0.2
4,505,147	-				-0.5
4,505,152	-				-0.5
4,505,152	-				-0.9
4,505,162	+				+1.1
4,505,162	+				-0.3
4,505,165	-				+1.0
4,505,169	-				-0.6
4,505,181	+				+0.9
4,505,181	+				-0.7
4,505,181	-				-0.5
4,505,181	-				+1.0
4,505,181	-				+0.2
4,505,191	+				+2.7
4,505,191	+				+1.7
4,505,196	+				-2.0
4,505,204	+				+0.1
4,505,204	-				+0.3
4,505,204	-				-0.9
4,505,204	-				+1.0
4,505,206	+				-1.3
4,505,207	-				+0.1
4,505,207	-				-0.9
4,505,209	-				-0.1
4,505,212	-				-0.2
4,505,267	+	insO	b4623	0.18	+0.9
4,505,282	+	insO	b4623	0.24	-1.0
4,505,288	-	insO	b4623	0.27	+0.2
4,505,290	-	insO	b4623	0.27	+0.5
4,505,290	-	insO	b4623	0.27	-0.8
4,505,290	-	insO	b4623	0.27	-0.8
4,505,311	-	insO	b4623	0.36	-0.0
4,505,311	-	insO	b4623	0.36	-0.4
4,505,327	+	insO	b4623	0.42	+0.7
4,505,334	-	insO	b4623	0.45	+0.3
4,505,335	-	insO	b4623	0.45	+0.7
4,505,336	+	insO	b4623	0.45	+0.5
4,505,346	+	insO	b4623	0.49	-0.1
4,505,349	+	insO	b4623	0.51	-0.9
4,505,349	+	insO	b4623	0.51	-1.3
4,505,357	+	insO	b4623	0.54	+1.1
4,505,357	+	insO	b4623	0.54	-0.2
4,505,357	-	insO	b4623	0.54	-1.0
4,505,362	-	insO	b4623	0.56	-0.4
4,505,399	-	insO	b4623	0.70	+0.1