Experiment: Isobutyl alcohol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflK and hflC are separated by 2 nucleotides hflC and yjeT are separated by 81 nucleotides yjeT and purA are separated by 103 nucleotides
b4174: hflK - modulator for HflB protease specific for phage lambda cII repressor (NCBI), at 4,400,061 to 4,401,320
hflK
b4175: hflC - modulator for HflB protease specific for phage lambda cII repressor (NCBI), at 4,401,323 to 4,402,327
hflC
b4176: yjeT - conserved inner membrane protein (NCBI), at 4,402,409 to 4,402,606
yjeT
b4177: purA - adenylosuccinate synthetase (NCBI), at 4,402,710 to 4,404,008
purA
Position (kb)
4401
4402
4403 Strain fitness (log2 ratio)
-2
-1
0
1 at 4400.356 kb on + strand, within hflK at 4400.385 kb on - strand, within hflK at 4400.385 kb on - strand, within hflK at 4400.411 kb on + strand, within hflK at 4400.416 kb on + strand, within hflK at 4400.416 kb on + strand, within hflK at 4400.429 kb on - strand, within hflK at 4400.476 kb on - strand, within hflK at 4400.502 kb on - strand, within hflK at 4400.534 kb on - strand, within hflK at 4400.546 kb on - strand, within hflK at 4400.555 kb on - strand, within hflK at 4400.582 kb on + strand, within hflK at 4400.586 kb on + strand, within hflK at 4400.586 kb on + strand, within hflK at 4400.620 kb on - strand, within hflK at 4400.643 kb on + strand, within hflK at 4400.654 kb on + strand, within hflK at 4400.704 kb on - strand, within hflK at 4400.704 kb on - strand, within hflK at 4400.709 kb on - strand, within hflK at 4400.716 kb on + strand, within hflK at 4400.716 kb on + strand, within hflK at 4400.747 kb on + strand, within hflK at 4400.749 kb on + strand, within hflK at 4400.767 kb on - strand, within hflK at 4400.779 kb on - strand, within hflK at 4400.779 kb on - strand, within hflK at 4400.860 kb on + strand, within hflK at 4400.875 kb on - strand, within hflK at 4400.908 kb on - strand, within hflK at 4400.932 kb on + strand, within hflK at 4400.933 kb on + strand, within hflK at 4400.942 kb on + strand, within hflK at 4400.942 kb on + strand, within hflK at 4400.942 kb on - strand, within hflK at 4400.951 kb on - strand, within hflK at 4400.951 kb on - strand, within hflK at 4400.952 kb on + strand, within hflK at 4400.952 kb on + strand, within hflK at 4400.952 kb on + strand, within hflK at 4400.966 kb on + strand, within hflK at 4400.983 kb on + strand, within hflK at 4400.991 kb on - strand, within hflK at 4401.008 kb on + strand, within hflK at 4401.010 kb on - strand, within hflK at 4401.073 kb on - strand, within hflK at 4401.091 kb on - strand, within hflK at 4401.091 kb on - strand, within hflK at 4401.218 kb on + strand at 4401.238 kb on - strand at 4401.238 kb on - strand at 4401.252 kb on - strand at 4401.259 kb on + strand at 4401.310 kb on + strand at 4401.362 kb on + strand at 4401.368 kb on + strand at 4401.368 kb on + strand at 4401.408 kb on + strand at 4401.408 kb on + strand at 4401.408 kb on + strand at 4401.408 kb on + strand at 4401.426 kb on + strand, within hflC at 4401.436 kb on + strand, within hflC at 4401.467 kb on - strand, within hflC at 4401.526 kb on + strand, within hflC at 4401.605 kb on - strand, within hflC at 4401.628 kb on + strand, within hflC at 4401.639 kb on - strand, within hflC at 4401.643 kb on - strand, within hflC at 4401.756 kb on - strand, within hflC at 4401.764 kb on - strand, within hflC at 4401.764 kb on - strand, within hflC at 4401.792 kb on - strand, within hflC at 4401.792 kb on - strand, within hflC at 4401.910 kb on + strand, within hflC at 4401.912 kb on + strand, within hflC at 4401.912 kb on + strand, within hflC at 4401.912 kb on + strand, within hflC at 4401.912 kb on + strand, within hflC at 4401.920 kb on - strand, within hflC at 4401.920 kb on - strand, within hflC at 4401.920 kb on - strand, within hflC at 4401.920 kb on - strand, within hflC at 4401.927 kb on + strand, within hflC at 4401.958 kb on + strand, within hflC at 4401.986 kb on - strand, within hflC at 4401.986 kb on - strand, within hflC at 4401.997 kb on - strand, within hflC at 4401.997 kb on - strand, within hflC at 4402.038 kb on + strand, within hflC at 4402.038 kb on + strand, within hflC at 4402.126 kb on - strand, within hflC at 4402.126 kb on - strand, within hflC at 4402.138 kb on - strand, within hflC at 4402.166 kb on - strand, within hflC at 4402.191 kb on - strand, within hflC at 4402.220 kb on - strand, within hflC at 4402.220 kb on - strand, within hflC at 4402.253 kb on - strand at 4402.253 kb on - strand at 4402.258 kb on + strand at 4402.263 kb on - strand at 4402.263 kb on - strand at 4402.263 kb on - strand at 4402.263 kb on - strand at 4402.326 kb on + strand at 4402.336 kb on + strand at 4402.336 kb on + strand at 4402.341 kb on + strand at 4402.373 kb on + strand at 4402.441 kb on + strand, within yjeT at 4402.449 kb on - strand, within yjeT at 4402.484 kb on - strand, within yjeT at 4402.497 kb on - strand, within yjeT at 4402.930 kb on - strand, within purA at 4403.242 kb on - strand, within purA
Per-strain Table
Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol% remove 4,400,356 + hflK b4174 0.23 +0.8 4,400,385 - hflK b4174 0.26 -0.3 4,400,385 - hflK b4174 0.26 +1.4 4,400,411 + hflK b4174 0.28 -0.1 4,400,416 + hflK b4174 0.28 -2.3 4,400,416 + hflK b4174 0.28 +0.5 4,400,429 - hflK b4174 0.29 +0.3 4,400,476 - hflK b4174 0.33 -0.1 4,400,502 - hflK b4174 0.35 -1.1 4,400,534 - hflK b4174 0.38 -0.9 4,400,546 - hflK b4174 0.38 -0.0 4,400,555 - hflK b4174 0.39 +0.0 4,400,582 + hflK b4174 0.41 +1.1 4,400,586 + hflK b4174 0.42 +0.8 4,400,586 + hflK b4174 0.42 -0.4 4,400,620 - hflK b4174 0.44 -1.3 4,400,643 + hflK b4174 0.46 +0.3 4,400,654 + hflK b4174 0.47 -0.3 4,400,704 - hflK b4174 0.51 +0.1 4,400,704 - hflK b4174 0.51 +1.3 4,400,709 - hflK b4174 0.51 -0.2 4,400,716 + hflK b4174 0.52 -0.7 4,400,716 + hflK b4174 0.52 -0.5 4,400,747 + hflK b4174 0.54 +0.3 4,400,749 + hflK b4174 0.55 +0.5 4,400,767 - hflK b4174 0.56 -0.1 4,400,779 - hflK b4174 0.57 +0.4 4,400,779 - hflK b4174 0.57 -0.3 4,400,860 + hflK b4174 0.63 -0.8 4,400,875 - hflK b4174 0.65 +0.8 4,400,908 - hflK b4174 0.67 +1.0 4,400,932 + hflK b4174 0.69 -0.8 4,400,933 + hflK b4174 0.69 +0.7 4,400,942 + hflK b4174 0.70 +0.7 4,400,942 + hflK b4174 0.70 +1.0 4,400,942 - hflK b4174 0.70 +1.0 4,400,951 - hflK b4174 0.71 -0.3 4,400,951 - hflK b4174 0.71 -1.1 4,400,952 + hflK b4174 0.71 +0.9 4,400,952 + hflK b4174 0.71 -0.6 4,400,952 + hflK b4174 0.71 -0.5 4,400,966 + hflK b4174 0.72 -0.3 4,400,983 + hflK b4174 0.73 -0.7 4,400,991 - hflK b4174 0.74 +0.2 4,401,008 + hflK b4174 0.75 -0.5 4,401,010 - hflK b4174 0.75 -0.4 4,401,073 - hflK b4174 0.80 +0.6 4,401,091 - hflK b4174 0.82 -0.0 4,401,091 - hflK b4174 0.82 -0.4 4,401,218 + -0.3 4,401,238 - -0.6 4,401,238 - -0.4 4,401,252 - +0.0 4,401,259 + -0.0 4,401,310 + -0.5 4,401,362 + +1.3 4,401,368 + +0.4 4,401,368 + +1.0 4,401,408 + -0.0 4,401,408 + +0.3 4,401,408 + +0.1 4,401,408 + +0.1 4,401,426 + hflC b4175 0.10 -0.3 4,401,436 + hflC b4175 0.11 +1.0 4,401,467 - hflC b4175 0.14 +0.9 4,401,526 + hflC b4175 0.20 -0.1 4,401,605 - hflC b4175 0.28 -0.1 4,401,628 + hflC b4175 0.30 +0.7 4,401,639 - hflC b4175 0.31 +0.2 4,401,643 - hflC b4175 0.32 -0.2 4,401,756 - hflC b4175 0.43 +0.5 4,401,764 - hflC b4175 0.44 -0.2 4,401,764 - hflC b4175 0.44 -0.3 4,401,792 - hflC b4175 0.47 +0.3 4,401,792 - hflC b4175 0.47 +1.4 4,401,910 + hflC b4175 0.58 +0.9 4,401,912 + hflC b4175 0.59 +0.1 4,401,912 + hflC b4175 0.59 +0.1 4,401,912 + hflC b4175 0.59 +0.3 4,401,912 + hflC b4175 0.59 +0.6 4,401,920 - hflC b4175 0.59 +0.9 4,401,920 - hflC b4175 0.59 +0.2 4,401,920 - hflC b4175 0.59 +0.5 4,401,920 - hflC b4175 0.59 -0.3 4,401,927 + hflC b4175 0.60 -0.3 4,401,958 + hflC b4175 0.63 +1.0 4,401,986 - hflC b4175 0.66 -0.2 4,401,986 - hflC b4175 0.66 +0.8 4,401,997 - hflC b4175 0.67 +0.3 4,401,997 - hflC b4175 0.67 +0.5 4,402,038 + hflC b4175 0.71 -0.6 4,402,038 + hflC b4175 0.71 +0.6 4,402,126 - hflC b4175 0.80 +0.2 4,402,126 - hflC b4175 0.80 -0.1 4,402,138 - hflC b4175 0.81 +0.1 4,402,166 - hflC b4175 0.84 +0.7 4,402,191 - hflC b4175 0.86 +1.1 4,402,220 - hflC b4175 0.89 +0.6 4,402,220 - hflC b4175 0.89 +0.7 4,402,253 - +0.0 4,402,253 - +0.3 4,402,258 + +0.3 4,402,263 - +0.2 4,402,263 - -0.8 4,402,263 - -0.2 4,402,263 - +0.7 4,402,326 + -0.3 4,402,336 + -0.2 4,402,336 + -0.2 4,402,341 + -0.0 4,402,373 + -0.6 4,402,441 + yjeT b4176 0.16 -0.1 4,402,449 - yjeT b4176 0.20 -0.8 4,402,484 - yjeT b4176 0.38 +0.1 4,402,497 - yjeT b4176 0.44 +0.4 4,402,930 - purA b4177 0.17 +1.3 4,403,242 - purA b4177 0.41 -1.6
Or see this region's nucleotide sequence