Strain Fitness in Escherichia coli BW25113 around b3462

Experiment: Isobutyl alcohol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoH and ftsX are separated by 244 nucleotidesftsX and ftsE overlap by 8 nucleotidesftsE and ftsY are separated by 2 nucleotides b3461: rpoH - RNA polymerase sigma factor (NCBI), at 3,597,952 to 3,598,806 rpoH b3462: ftsX - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 3,599,051 to 3,600,109 ftsX b3463: ftsE - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 3,600,102 to 3,600,770 ftsE b3464: ftsY - fused Signal Recognition Particle (SRP) receptor: membrane binding protein/conserved protein (NCBI), at 3,600,773 to 3,602,266 ftsY Position (kb) 3599 3600 3601Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3598.735 kb on - strandat 3598.802 kb on + strandat 3598.802 kb on + strandat 3599.058 kb on + strandat 3599.058 kb on + strandat 3599.066 kb on - strandat 3599.093 kb on - strandat 3599.093 kb on - strandat 3599.283 kb on - strand, within ftsXat 3599.294 kb on - strand, within ftsXat 3599.340 kb on - strand, within ftsXat 3599.655 kb on + strand, within ftsXat 3599.655 kb on + strand, within ftsXat 3599.720 kb on - strand, within ftsXat 3599.722 kb on - strand, within ftsXat 3599.795 kb on + strand, within ftsXat 3599.832 kb on + strand, within ftsXat 3599.840 kb on - strand, within ftsXat 3599.945 kb on - strand, within ftsXat 3599.945 kb on - strand, within ftsXat 3599.972 kb on - strand, within ftsXat 3600.020 kb on + strandat 3600.042 kb on + strandat 3600.042 kb on + strandat 3600.106 kb on - strandat 3600.117 kb on + strandat 3600.126 kb on - strandat 3600.126 kb on - strandat 3600.145 kb on - strandat 3600.147 kb on - strandat 3600.147 kb on - strandat 3600.157 kb on - strandat 3600.184 kb on - strand, within ftsEat 3600.286 kb on + strand, within ftsEat 3600.304 kb on + strand, within ftsEat 3600.309 kb on + strand, within ftsEat 3600.362 kb on + strand, within ftsEat 3600.392 kb on - strand, within ftsEat 3600.412 kb on + strand, within ftsEat 3600.451 kb on + strand, within ftsEat 3600.485 kb on - strand, within ftsEat 3600.490 kb on - strand, within ftsEat 3600.604 kb on + strand, within ftsEat 3600.612 kb on - strand, within ftsEat 3600.692 kb on + strand, within ftsEat 3600.692 kb on + strand, within ftsEat 3600.719 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol%
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3,598,735 - +0.0
3,598,802 + -2.5
3,598,802 + -1.3
3,599,058 + +0.4
3,599,058 + -0.2
3,599,066 - +0.1
3,599,093 - +0.2
3,599,093 - +1.6
3,599,283 - ftsX b3462 0.22 +1.8
3,599,294 - ftsX b3462 0.23 +0.8
3,599,340 - ftsX b3462 0.27 +1.8
3,599,655 + ftsX b3462 0.57 +1.8
3,599,655 + ftsX b3462 0.57 +1.8
3,599,720 - ftsX b3462 0.63 +1.8
3,599,722 - ftsX b3462 0.63 +1.8
3,599,795 + ftsX b3462 0.70 +1.8
3,599,832 + ftsX b3462 0.74 +0.8
3,599,840 - ftsX b3462 0.75 +1.8
3,599,945 - ftsX b3462 0.84 +0.4
3,599,945 - ftsX b3462 0.84 +0.4
3,599,972 - ftsX b3462 0.87 +0.6
3,600,020 + +1.8
3,600,042 + +0.0
3,600,042 + +0.9
3,600,106 - +0.1
3,600,117 + +1.8
3,600,126 - +0.7
3,600,126 - +0.1
3,600,145 - +0.4
3,600,147 - +0.2
3,600,147 - +2.8
3,600,157 - +1.8
3,600,184 - ftsE b3463 0.12 +1.8
3,600,286 + ftsE b3463 0.28 +1.8
3,600,304 + ftsE b3463 0.30 +1.8
3,600,309 + ftsE b3463 0.31 +0.8
3,600,362 + ftsE b3463 0.39 +1.8
3,600,392 - ftsE b3463 0.43 +1.8
3,600,412 + ftsE b3463 0.46 +1.8
3,600,451 + ftsE b3463 0.52 +1.8
3,600,485 - ftsE b3463 0.57 +1.8
3,600,490 - ftsE b3463 0.58 +1.8
3,600,604 + ftsE b3463 0.75 +1.8
3,600,612 - ftsE b3463 0.76 +0.8
3,600,692 + ftsE b3463 0.88 +1.8
3,600,692 + ftsE b3463 0.88 +1.8
3,600,719 - +1.8

Or see this region's nucleotide sequence