Strain Fitness in Escherichia coli BW25113 around b2477

Experiment: Isobutyl alcohol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurC and nlpB are separated by 212 nucleotidesnlpB and dapA are separated by 16 nucleotides b2476: purC - phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI), at 2,594,927 to 2,595,640 purC b2477: nlpB - lipoprotein-34 (VIMSS), at 2,595,853 to 2,596,887 nlpB b2478: dapA - dihydrodipicolinate synthase (NCBI), at 2,596,904 to 2,597,782 dapA Position (kb) 2595 2596 2597Strain fitness (log2 ratio) -2 -1 0 1at 2594.872 kb on - strandat 2594.943 kb on - strandat 2594.980 kb on + strandat 2595.179 kb on - strandat 2595.179 kb on - strand, within purCat 2595.364 kb on - strand, within purCat 2595.494 kb on + strand, within purCat 2595.574 kb on + strandat 2595.593 kb on + strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.601 kb on - strandat 2595.625 kb on - strandat 2595.837 kb on + strandat 2595.877 kb on - strandat 2595.937 kb on + strandat 2595.937 kb on + strandat 2595.944 kb on - strandat 2595.944 kb on - strandat 2595.991 kb on + strand, within nlpBat 2595.991 kb on + strand, within nlpBat 2596.206 kb on + strand, within nlpBat 2596.295 kb on - strand, within nlpBat 2596.349 kb on - strand, within nlpBat 2596.439 kb on - strand, within nlpBat 2596.441 kb on + strand, within nlpBat 2596.792 kb on + strandat 2596.792 kb on + strandat 2596.883 kb on - strandat 2596.883 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol%
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2,594,872 - -2.1
2,594,943 - -0.3
2,594,980 + -2.4
2,595,179 - -0.2
2,595,179 - purC b2476 0.35 +1.4
2,595,364 - purC b2476 0.61 -0.5
2,595,494 + purC b2476 0.79 +0.5
2,595,574 + +0.3
2,595,593 + -0.4
2,595,601 - +0.2
2,595,601 - +0.4
2,595,601 - -0.9
2,595,601 - +0.0
2,595,601 - +0.6
2,595,625 - +0.1
2,595,837 + -0.2
2,595,877 - +0.2
2,595,937 + -2.0
2,595,937 + +0.1
2,595,944 - -0.5
2,595,944 - +1.4
2,595,991 + nlpB b2477 0.13 +0.8
2,595,991 + nlpB b2477 0.13 +1.0
2,596,206 + nlpB b2477 0.34 +0.8
2,596,295 - nlpB b2477 0.43 +0.2
2,596,349 - nlpB b2477 0.48 +0.0
2,596,439 - nlpB b2477 0.57 -0.2
2,596,441 + nlpB b2477 0.57 -0.4
2,596,792 + -1.5
2,596,792 + -0.3
2,596,883 - +0.4
2,596,883 - +0.3

Or see this region's nucleotide sequence