Strain Fitness in Escherichia coli BW25113 around b2182

Experiment: Isobutyl alcohol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyejF and yejG are separated by 3 nucleotidesyejG and bcr are separated by 332 nucleotidesbcr and rsuA are separated by 27 nucleotidesrsuA and yejH are separated by 148 nucleotides b2180: yejF - fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI), at 2,274,322 to 2,275,911 yejF b2181: yejG - hypothetical protein (NCBI), at 2,275,915 to 2,276,259 yejG b2182: bcr - bicyclomycin/multidrug efflux system (NCBI), at 2,276,592 to 2,277,782 bcr b2183: rsuA - 16S rRNA pseudouridylate 516 synthase (NCBI), at 2,277,810 to 2,278,505 rsuA b2184: yejH - DNA damage response helicase (yejH or radD) (from data), at 2,278,654 to 2,280,414 yejH Position (kb) 2276 2277 2278Strain fitness (log2 ratio) -1 0 1 2at 2275.673 kb on + strand, within yejFat 2275.814 kb on - strandat 2275.816 kb on - strandat 2275.870 kb on + strandat 2275.870 kb on + strandat 2276.009 kb on + strand, within yejGat 2276.027 kb on - strand, within yejGat 2276.032 kb on + strand, within yejGat 2276.032 kb on + strand, within yejGat 2276.032 kb on + strand, within yejGat 2276.032 kb on - strand, within yejGat 2276.078 kb on + strand, within yejGat 2276.099 kb on + strand, within yejGat 2276.108 kb on - strand, within yejGat 2276.245 kb on - strandat 2276.332 kb on + strandat 2276.374 kb on + strandat 2276.406 kb on - strandat 2276.411 kb on - strandat 2276.411 kb on - strandat 2276.429 kb on - strandat 2276.885 kb on - strand, within bcrat 2276.892 kb on - strand, within bcrat 2276.892 kb on - strand, within bcrat 2276.950 kb on - strand, within bcrat 2276.950 kb on - strand, within bcrat 2277.132 kb on + strand, within bcrat 2277.137 kb on + strand, within bcrat 2277.197 kb on + strand, within bcrat 2277.287 kb on + strand, within bcrat 2277.287 kb on + strand, within bcrat 2277.352 kb on - strand, within bcrat 2277.352 kb on - strand, within bcrat 2277.352 kb on - strand, within bcrat 2277.422 kb on - strand, within bcrat 2277.445 kb on + strand, within bcrat 2277.573 kb on + strand, within bcrat 2277.625 kb on - strand, within bcrat 2277.628 kb on - strand, within bcrat 2277.641 kb on + strand, within bcrat 2277.695 kb on + strandat 2277.695 kb on + strandat 2277.696 kb on + strandat 2277.759 kb on + strandat 2277.767 kb on + strandat 2278.002 kb on - strand, within rsuAat 2278.018 kb on - strand, within rsuAat 2278.126 kb on - strand, within rsuAat 2278.173 kb on + strand, within rsuAat 2278.266 kb on - strand, within rsuAat 2278.285 kb on - strand, within rsuAat 2278.462 kb on - strandat 2278.560 kb on - strandat 2278.678 kb on - strandat 2278.720 kb on + strandat 2278.720 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol%
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2,275,673 + yejF b2180 0.85 -0.1
2,275,814 - +0.2
2,275,816 - +0.3
2,275,870 + +0.0
2,275,870 + -0.7
2,276,009 + yejG b2181 0.27 +0.0
2,276,027 - yejG b2181 0.32 -0.2
2,276,032 + yejG b2181 0.34 +0.7
2,276,032 + yejG b2181 0.34 -1.4
2,276,032 + yejG b2181 0.34 +2.7
2,276,032 - yejG b2181 0.34 +0.1
2,276,078 + yejG b2181 0.47 -0.4
2,276,099 + yejG b2181 0.53 -0.5
2,276,108 - yejG b2181 0.56 -0.3
2,276,245 - -0.3
2,276,332 + +0.5
2,276,374 + -0.9
2,276,406 - -0.9
2,276,411 - +0.5
2,276,411 - -1.1
2,276,429 - -0.6
2,276,885 - bcr b2182 0.25 +0.7
2,276,892 - bcr b2182 0.25 +0.6
2,276,892 - bcr b2182 0.25 +0.2
2,276,950 - bcr b2182 0.30 +1.7
2,276,950 - bcr b2182 0.30 +1.5
2,277,132 + bcr b2182 0.45 +0.7
2,277,137 + bcr b2182 0.46 +0.2
2,277,197 + bcr b2182 0.51 +0.1
2,277,287 + bcr b2182 0.58 +1.2
2,277,287 + bcr b2182 0.58 +0.2
2,277,352 - bcr b2182 0.64 -0.6
2,277,352 - bcr b2182 0.64 -0.9
2,277,352 - bcr b2182 0.64 +0.0
2,277,422 - bcr b2182 0.70 +1.0
2,277,445 + bcr b2182 0.72 -0.1
2,277,573 + bcr b2182 0.82 +0.1
2,277,625 - bcr b2182 0.87 -0.4
2,277,628 - bcr b2182 0.87 -0.1
2,277,641 + bcr b2182 0.88 +0.1
2,277,695 + +0.3
2,277,695 + -0.1
2,277,696 + +2.0
2,277,759 + -0.5
2,277,767 + +0.2
2,278,002 - rsuA b2183 0.28 +0.6
2,278,018 - rsuA b2183 0.30 -1.0
2,278,126 - rsuA b2183 0.45 -1.2
2,278,173 + rsuA b2183 0.52 +1.0
2,278,266 - rsuA b2183 0.66 -0.6
2,278,285 - rsuA b2183 0.68 +0.2
2,278,462 - -0.1
2,278,560 - +0.1
2,278,678 - +0.1
2,278,720 + -0.3
2,278,720 + +0.3

Or see this region's nucleotide sequence