Strain Fitness in Escherichia coli BW25113 around b1601

Experiment: Isobutyl alcohol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdtI and mdtJ overlap by 14 nucleotidesmdtJ and ydgG are separated by 411 nucleotidesydgG and pntB are separated by 24 nucleotides b1599: mdtI - multidrug efflux system transporter (NCBI), at 1,670,844 to 1,671,173 mdtI b1600: mdtJ - multidrug efflux system transporter (NCBI), at 1,671,160 to 1,671,525 mdtJ b1601: ydgG - predicted inner membrane protein (NCBI), at 1,671,937 to 1,672,971 ydgG b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384 pntB Position (kb) 1671 1672 1673Strain fitness (log2 ratio) -1 0 1 2at 1671.156 kb on - strandat 1671.156 kb on - strandat 1671.165 kb on - strandat 1671.182 kb on - strandat 1671.186 kb on + strandat 1671.251 kb on - strand, within mdtJat 1671.253 kb on - strand, within mdtJat 1671.346 kb on + strand, within mdtJat 1671.348 kb on - strand, within mdtJat 1671.348 kb on - strand, within mdtJat 1671.354 kb on - strand, within mdtJat 1671.354 kb on - strand, within mdtJat 1671.355 kb on - strand, within mdtJat 1671.449 kb on - strand, within mdtJat 1671.466 kb on - strand, within mdtJat 1671.466 kb on - strand, within mdtJat 1671.474 kb on - strand, within mdtJat 1671.525 kb on - strandat 1671.595 kb on - strandat 1671.615 kb on - strandat 1671.653 kb on - strandat 1671.653 kb on - strandat 1671.653 kb on - strandat 1671.653 kb on - strandat 1671.665 kb on + strandat 1671.761 kb on - strandat 1671.761 kb on - strandat 1671.761 kb on - strandat 1671.800 kb on - strandat 1671.800 kb on - strandat 1671.927 kb on + strandat 1671.935 kb on + strandat 1671.941 kb on - strandat 1671.943 kb on - strandat 1672.027 kb on + strandat 1672.082 kb on - strand, within ydgGat 1672.082 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.095 kb on - strand, within ydgGat 1672.102 kb on - strand, within ydgGat 1672.106 kb on + strand, within ydgGat 1672.151 kb on - strand, within ydgGat 1672.157 kb on - strand, within ydgGat 1672.291 kb on - strand, within ydgGat 1672.365 kb on + strand, within ydgGat 1672.365 kb on + strand, within ydgGat 1672.413 kb on + strand, within ydgGat 1672.481 kb on + strand, within ydgGat 1672.668 kb on - strand, within ydgGat 1672.698 kb on - strand, within ydgGat 1672.698 kb on - strand, within ydgGat 1672.796 kb on + strand, within ydgGat 1673.042 kb on + strandat 1673.095 kb on - strandat 1673.102 kb on - strandat 1673.145 kb on + strand, within pntBat 1673.501 kb on - strand, within pntBat 1673.579 kb on - strand, within pntBat 1673.579 kb on - strand, within pntBat 1673.667 kb on + strand, within pntBat 1673.672 kb on + strand, within pntBat 1673.672 kb on + strand, within pntBat 1673.878 kb on + strand, within pntBat 1673.878 kb on + strand, within pntB

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Per-strain Table

Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol%
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1,671,156 - +0.2
1,671,156 - -0.0
1,671,165 - -1.2
1,671,182 - +0.2
1,671,186 + +0.9
1,671,251 - mdtJ b1600 0.25 +2.5
1,671,253 - mdtJ b1600 0.25 +1.9
1,671,346 + mdtJ b1600 0.51 +0.9
1,671,348 - mdtJ b1600 0.51 +0.9
1,671,348 - mdtJ b1600 0.51 +0.9
1,671,354 - mdtJ b1600 0.53 -0.1
1,671,354 - mdtJ b1600 0.53 -1.4
1,671,355 - mdtJ b1600 0.53 +2.1
1,671,449 - mdtJ b1600 0.79 +0.9
1,671,466 - mdtJ b1600 0.84 +0.9
1,671,466 - mdtJ b1600 0.84 +1.3
1,671,474 - mdtJ b1600 0.86 +0.9
1,671,525 - +0.9
1,671,595 - -0.6
1,671,615 - -1.4
1,671,653 - -1.1
1,671,653 - -1.1
1,671,653 - +1.3
1,671,653 - -1.1
1,671,665 + +0.9
1,671,761 - +0.8
1,671,761 - +0.7
1,671,761 - -0.6
1,671,800 - -0.3
1,671,800 - -0.6
1,671,927 + +0.9
1,671,935 + +0.9
1,671,941 - -1.4
1,671,943 - +0.9
1,672,027 + +1.7
1,672,082 - ydgG b1601 0.14 +1.3
1,672,082 - ydgG b1601 0.14 +0.1
1,672,095 - ydgG b1601 0.15 +0.9
1,672,095 - ydgG b1601 0.15 +0.2
1,672,095 - ydgG b1601 0.15 -1.4
1,672,102 - ydgG b1601 0.16 -0.8
1,672,106 + ydgG b1601 0.16 +0.9
1,672,151 - ydgG b1601 0.21 +1.0
1,672,157 - ydgG b1601 0.21 +1.2
1,672,291 - ydgG b1601 0.34 -0.1
1,672,365 + ydgG b1601 0.41 +1.1
1,672,365 + ydgG b1601 0.41 +0.6
1,672,413 + ydgG b1601 0.46 -0.2
1,672,481 + ydgG b1601 0.53 +0.4
1,672,668 - ydgG b1601 0.71 +1.6
1,672,698 - ydgG b1601 0.74 +0.6
1,672,698 - ydgG b1601 0.74 -0.3
1,672,796 + ydgG b1601 0.83 +0.4
1,673,042 + +0.5
1,673,095 - -0.1
1,673,102 - +0.2
1,673,145 + pntB b1602 0.11 +0.5
1,673,501 - pntB b1602 0.36 -0.3
1,673,579 - pntB b1602 0.42 +0.7
1,673,579 - pntB b1602 0.42 -1.2
1,673,667 + pntB b1602 0.48 -0.6
1,673,672 + pntB b1602 0.49 -0.3
1,673,672 + pntB b1602 0.49 +0.7
1,673,878 + pntB b1602 0.63 +0.3
1,673,878 + pntB b1602 0.63 -0.8

Or see this region's nucleotide sequence