Strain Fitness in Escherichia coli BW25113 around b0739

Experiment: Isobutyl alcohol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolQ and tolR are separated by 3 nucleotidestolR and tolA are separated by 64 nucleotidestolA and tolB are separated by 132 nucleotides b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068 tolQ b0738: tolR - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 775,072 to 775,500 tolR b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830 tolA b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255 tolB Position (kb) 775 776 777Strain fitness (log2 ratio) -2 -1 0 1 2 3at 774.571 kb on + strand, within tolQat 774.649 kb on + strand, within tolQat 774.668 kb on - strand, within tolQat 774.723 kb on + strand, within tolQat 774.723 kb on + strand, within tolQat 774.809 kb on - strand, within tolQat 774.813 kb on - strand, within tolQat 774.818 kb on - strand, within tolQat 774.839 kb on + strand, within tolQat 774.905 kb on + strand, within tolQat 774.956 kb on + strand, within tolQat 775.063 kb on + strandat 775.063 kb on + strandat 775.070 kb on - strandat 775.071 kb on - strandat 775.071 kb on - strandat 775.091 kb on - strandat 775.121 kb on + strand, within tolRat 775.121 kb on + strand, within tolRat 775.121 kb on + strand, within tolRat 775.146 kb on + strand, within tolRat 775.152 kb on - strand, within tolRat 775.194 kb on - strand, within tolRat 775.258 kb on + strand, within tolRat 775.265 kb on + strand, within tolRat 775.275 kb on + strand, within tolRat 775.280 kb on + strand, within tolRat 775.288 kb on - strand, within tolRat 775.288 kb on - strand, within tolRat 775.298 kb on - strand, within tolRat 775.333 kb on + strand, within tolRat 775.333 kb on + strand, within tolRat 775.357 kb on - strand, within tolRat 775.526 kb on - strandat 775.533 kb on + strandat 775.565 kb on + strandat 775.628 kb on + strandat 775.651 kb on + strandat 775.659 kb on - strandat 775.672 kb on + strandat 775.748 kb on + strand, within tolAat 775.753 kb on + strand, within tolAat 775.840 kb on + strand, within tolAat 775.930 kb on - strand, within tolAat 775.932 kb on + strand, within tolAat 775.947 kb on + strand, within tolAat 776.098 kb on - strand, within tolAat 776.124 kb on + strand, within tolAat 776.146 kb on + strand, within tolAat 776.265 kb on - strand, within tolAat 776.265 kb on - strand, within tolAat 776.464 kb on + strand, within tolAat 776.475 kb on - strand, within tolAat 776.601 kb on - strand, within tolAat 776.625 kb on - strand, within tolAat 776.640 kb on - strand, within tolAat 776.640 kb on - strand, within tolAat 776.657 kb on - strand, within tolAat 776.713 kb on - strandat 776.725 kb on + strandat 776.779 kb on - strandat 776.943 kb on + strandat 776.968 kb on - strandat 776.994 kb on + strandat 776.994 kb on + strandat 777.012 kb on + strandat 777.014 kb on + strandat 777.135 kb on + strand, within tolBat 777.146 kb on + strand, within tolBat 777.260 kb on + strand, within tolBat 777.278 kb on - strand, within tolBat 777.299 kb on - strand, within tolBat 777.303 kb on - strand, within tolBat 777.393 kb on - strand, within tolBat 777.398 kb on - strand, within tolBat 777.444 kb on - strand, within tolBat 777.513 kb on + strand, within tolBat 777.522 kb on - strand, within tolBat 777.602 kb on - strand, within tolBat 777.602 kb on - strand, within tolBat 777.692 kb on + strand, within tolBat 777.700 kb on - strand, within tolBat 777.720 kb on - strand, within tolBat 777.770 kb on - strand, within tolBat 777.792 kb on - strand, within tolB

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Per-strain Table

Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol%
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774,571 + tolQ b0737 0.28 +1.3
774,649 + tolQ b0737 0.39 -2.3
774,668 - tolQ b0737 0.42 +0.3
774,723 + tolQ b0737 0.50 +1.8
774,723 + tolQ b0737 0.50 +0.7
774,809 - tolQ b0737 0.62 +0.7
774,813 - tolQ b0737 0.63 +0.1
774,818 - tolQ b0737 0.64 +1.3
774,839 + tolQ b0737 0.67 +1.3
774,905 + tolQ b0737 0.76 -1.2
774,956 + tolQ b0737 0.84 +0.3
775,063 + +0.2
775,063 + +0.2
775,070 - -0.5
775,071 - +0.1
775,071 - -0.2
775,091 - +1.3
775,121 + tolR b0738 0.11 +1.1
775,121 + tolR b0738 0.11 +2.1
775,121 + tolR b0738 0.11 +1.3
775,146 + tolR b0738 0.17 -0.4
775,152 - tolR b0738 0.19 -0.3
775,194 - tolR b0738 0.28 +2.3
775,258 + tolR b0738 0.43 +0.3
775,265 + tolR b0738 0.45 +0.3
775,275 + tolR b0738 0.47 +1.3
775,280 + tolR b0738 0.48 -1.5
775,288 - tolR b0738 0.50 +0.3
775,288 - tolR b0738 0.50 +0.9
775,298 - tolR b0738 0.53 -0.5
775,333 + tolR b0738 0.61 +0.7
775,333 + tolR b0738 0.61 -0.5
775,357 - tolR b0738 0.66 -0.3
775,526 - +0.5
775,533 + +0.4
775,565 + -0.7
775,628 + +0.3
775,651 + +0.3
775,659 - +1.3
775,672 + -1.3
775,748 + tolA b0739 0.14 +0.6
775,753 + tolA b0739 0.15 +1.3
775,840 + tolA b0739 0.22 +1.0
775,930 - tolA b0739 0.29 +0.9
775,932 + tolA b0739 0.29 +0.6
775,947 + tolA b0739 0.30 +2.3
776,098 - tolA b0739 0.42 -0.3
776,124 + tolA b0739 0.44 +1.9
776,146 + tolA b0739 0.46 -0.7
776,265 - tolA b0739 0.55 +1.8
776,265 - tolA b0739 0.55 +0.7
776,464 + tolA b0739 0.71 -1.4
776,475 - tolA b0739 0.72 +1.1
776,601 - tolA b0739 0.82 +0.3
776,625 - tolA b0739 0.84 +1.3
776,640 - tolA b0739 0.85 +1.3
776,640 - tolA b0739 0.85 -0.5
776,657 - tolA b0739 0.86 -0.7
776,713 - -0.3
776,725 + -0.3
776,779 - -0.5
776,943 + -0.2
776,968 - -0.4
776,994 + -2.2
776,994 + +0.3
777,012 + +1.9
777,014 + -0.3
777,135 + tolB b0740 0.13 -0.0
777,146 + tolB b0740 0.14 +0.6
777,260 + tolB b0740 0.23 +3.1
777,278 - tolB b0740 0.24 -0.3
777,299 - tolB b0740 0.26 +0.4
777,303 - tolB b0740 0.26 -2.3
777,393 - tolB b0740 0.33 +1.3
777,398 - tolB b0740 0.34 +0.3
777,444 - tolB b0740 0.37 -0.4
777,513 + tolB b0740 0.43 +1.3
777,522 - tolB b0740 0.43 +0.3
777,602 - tolB b0740 0.49 -0.7
777,602 - tolB b0740 0.49 -2.5
777,692 + tolB b0740 0.56 -0.7
777,700 - tolB b0740 0.57 +2.1
777,720 - tolB b0740 0.59 -1.4
777,770 - tolB b0740 0.62 -2.0
777,792 - tolB b0740 0.64 +0.5

Or see this region's nucleotide sequence