Experiment: Isobutyl alcohol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhC and sdhD overlap by 7 nucleotides sdhD and sdhA overlap by 1 nucleotides sdhA and sdhB are separated by 15 nucleotides
b0721: sdhC - succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI), at 754,400 to 754,789
sdhC
b0722: sdhD - succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI), at 754,783 to 755,130
sdhD
b0723: sdhA - succinate dehydrogenase flavoprotein subunit (NCBI), at 755,130 to 756,896
sdhA
b0724: sdhB - succinate dehydrogenase, FeS subunit (NCBI), at 756,912 to 757,628
sdhB
Position (kb) 755
756
757 Strain fitness (log2 ratio) -1
0
1
2 at 754.174 kb on + strand at 754.209 kb on + strand at 754.305 kb on + strand at 754.305 kb on + strand at 754.385 kb on + strand at 754.477 kb on - strand, within sdhC at 754.507 kb on - strand, within sdhC at 754.512 kb on + strand, within sdhC at 754.550 kb on + strand, within sdhC at 754.636 kb on - strand, within sdhC at 754.663 kb on - strand, within sdhC at 754.715 kb on + strand, within sdhC at 754.759 kb on - strand at 754.775 kb on - strand at 754.802 kb on + strand at 754.809 kb on + strand at 754.909 kb on - strand, within sdhD at 754.950 kb on + strand, within sdhD at 754.966 kb on - strand, within sdhD at 754.977 kb on + strand, within sdhD at 754.990 kb on + strand, within sdhD at 755.014 kb on - strand, within sdhD at 755.019 kb on + strand, within sdhD at 755.045 kb on + strand, within sdhD at 755.083 kb on - strand, within sdhD at 755.098 kb on + strand at 755.115 kb on + strand at 755.115 kb on + strand at 755.116 kb on - strand at 755.290 kb on + strand at 755.301 kb on + strand at 755.320 kb on + strand, within sdhA at 755.323 kb on - strand, within sdhA at 755.344 kb on - strand, within sdhA at 755.353 kb on + strand, within sdhA at 755.366 kb on - strand, within sdhA at 755.368 kb on - strand, within sdhA at 755.382 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.481 kb on - strand, within sdhA at 755.491 kb on - strand, within sdhA at 755.554 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.564 kb on + strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.572 kb on + strand, within sdhA at 755.625 kb on + strand, within sdhA at 755.626 kb on - strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.663 kb on + strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.697 kb on - strand, within sdhA at 755.711 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.800 kb on + strand, within sdhA at 755.839 kb on + strand at 755.842 kb on - strand, within sdhA at 755.961 kb on - strand, within sdhA at 756.184 kb on + strand, within sdhA at 756.390 kb on - strand, within sdhA at 756.390 kb on - strand, within sdhA at 756.455 kb on - strand, within sdhA at 756.455 kb on - strand, within sdhA at 756.500 kb on + strand, within sdhA at 756.500 kb on + strand, within sdhA at 756.642 kb on + strand, within sdhA at 756.677 kb on + strand, within sdhA at 756.680 kb on - strand, within sdhA at 756.685 kb on - strand at 756.839 kb on + strand at 756.856 kb on - strand at 757.053 kb on + strand, within sdhB at 757.138 kb on - strand, within sdhB at 757.143 kb on - strand, within sdhB at 757.184 kb on - strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.229 kb on - strand, within sdhB at 757.233 kb on + strand, within sdhB at 757.241 kb on - strand, within sdhB at 757.381 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.488 kb on + strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.536 kb on + strand at 757.536 kb on + strand, within sdhB at 757.536 kb on - strand, within sdhB at 757.544 kb on + strand, within sdhB at 757.544 kb on - strand, within sdhB at 757.566 kb on - strand at 757.566 kb on - strand at 757.673 kb on + strand at 757.681 kb on - strand at 757.703 kb on + strand at 757.713 kb on - strand at 757.713 kb on - strand at 757.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol% remove 754,174 + +0.8 754,209 + +1.1 754,305 + +0.4 754,305 + -0.9 754,385 + +1.1 754,477 - sdhC b0721 0.20 -0.2 754,507 - sdhC b0721 0.27 +1.1 754,512 + sdhC b0721 0.29 +0.4 754,550 + sdhC b0721 0.38 +0.7 754,636 - sdhC b0721 0.61 +1.8 754,663 - sdhC b0721 0.67 +0.9 754,715 + sdhC b0721 0.81 +0.7 754,759 - +1.4 754,775 - +1.1 754,802 + +0.4 754,809 + +0.9 754,909 - sdhD b0722 0.36 +1.2 754,950 + sdhD b0722 0.48 +0.4 754,966 - sdhD b0722 0.53 +0.7 754,977 + sdhD b0722 0.56 -0.4 754,990 + sdhD b0722 0.59 -0.3 755,014 - sdhD b0722 0.66 +0.4 755,019 + sdhD b0722 0.68 +0.4 755,045 + sdhD b0722 0.75 +0.4 755,083 - sdhD b0722 0.86 +0.8 755,098 + -0.3 755,115 + +0.1 755,115 + +0.5 755,116 - +1.6 755,290 + +0.7 755,301 + +0.9 755,320 + sdhA b0723 0.11 +0.8 755,323 - sdhA b0723 0.11 +1.5 755,344 - sdhA b0723 0.12 +1.6 755,353 + sdhA b0723 0.13 +0.9 755,366 - sdhA b0723 0.13 +0.4 755,368 - sdhA b0723 0.13 +0.7 755,382 + sdhA b0723 0.14 +1.2 755,424 + sdhA b0723 0.17 +0.9 755,424 + sdhA b0723 0.17 +0.7 755,461 - sdhA b0723 0.19 +1.5 755,461 - sdhA b0723 0.19 +1.1 755,481 - sdhA b0723 0.20 +0.5 755,491 - sdhA b0723 0.20 +0.8 755,554 + sdhA b0723 0.24 +1.3 755,562 + sdhA b0723 0.24 +0.6 755,562 + sdhA b0723 0.24 +0.9 755,562 + sdhA b0723 0.24 +0.8 755,564 + sdhA b0723 0.25 +1.0 755,570 - sdhA b0723 0.25 +1.3 755,570 - sdhA b0723 0.25 +1.4 755,570 - sdhA b0723 0.25 +0.8 755,572 + sdhA b0723 0.25 +1.3 755,625 + sdhA b0723 0.28 +1.0 755,626 - sdhA b0723 0.28 +1.1 755,658 + sdhA b0723 0.30 +0.8 755,658 + sdhA b0723 0.30 +1.3 755,663 + sdhA b0723 0.30 +1.9 755,676 - sdhA b0723 0.31 +1.8 755,676 - sdhA b0723 0.31 +1.8 755,676 - sdhA b0723 0.31 +1.3 755,697 - sdhA b0723 0.32 +0.3 755,711 + sdhA b0723 0.33 +0.5 755,759 + sdhA b0723 0.36 +0.2 755,759 + sdhA b0723 0.36 -0.3 755,764 + sdhA b0723 0.36 +1.3 755,764 + sdhA b0723 0.36 +1.4 755,800 + sdhA b0723 0.38 +0.8 755,839 + +0.5 755,842 - sdhA b0723 0.40 +1.1 755,961 - sdhA b0723 0.47 +0.8 756,184 + sdhA b0723 0.60 +0.5 756,390 - sdhA b0723 0.71 +1.1 756,390 - sdhA b0723 0.71 +0.6 756,455 - sdhA b0723 0.75 +1.0 756,455 - sdhA b0723 0.75 +0.5 756,500 + sdhA b0723 0.78 +0.4 756,500 + sdhA b0723 0.78 +0.4 756,642 + sdhA b0723 0.86 -0.2 756,677 + sdhA b0723 0.88 +0.9 756,680 - sdhA b0723 0.88 +0.4 756,685 - -0.7 756,839 + -0.4 756,856 - +1.4 757,053 + sdhB b0724 0.20 +0.6 757,138 - sdhB b0724 0.32 +1.8 757,143 - sdhB b0724 0.32 -0.4 757,184 - sdhB b0724 0.38 +1.2 757,198 + sdhB b0724 0.40 +0.4 757,198 + sdhB b0724 0.40 +0.2 757,229 - sdhB b0724 0.44 +1.0 757,233 + sdhB b0724 0.45 +0.6 757,241 - sdhB b0724 0.46 +1.1 757,381 - sdhB b0724 0.65 +0.6 757,392 - sdhB b0724 0.67 +1.6 757,392 - sdhB b0724 0.67 +0.9 757,488 + sdhB b0724 0.80 +0.1 757,517 - sdhB b0724 0.84 +0.6 757,517 - sdhB b0724 0.84 +1.8 757,536 + -0.7 757,536 + sdhB b0724 0.87 +0.1 757,536 - sdhB b0724 0.87 +0.7 757,544 + sdhB b0724 0.88 +0.4 757,544 - sdhB b0724 0.88 +0.8 757,566 - +2.0 757,566 - +1.1 757,673 + -0.9 757,681 - +0.9 757,703 + +0.2 757,713 - +0.9 757,713 - +0.9 757,791 + +0.2
Or see this region's nucleotide sequence