Strain Fitness in Escherichia coli BW25113 around b0530

Experiment: Isobutyl alcohol 1.25 vol%

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntfolD and sfmA are separated by 470 nucleotidessfmA and sfmC are separated by 219 nucleotidessfmC and sfmD are separated by 30 nucleotides b0529: folD - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase (NCBI), at 556,098 to 556,964 folD b0530: sfmA - putative fimbrial-like protein (VIMSS), at 557,435 to 557,977 sfmA b0531: sfmC - pilin chaperone, periplasmic (NCBI), at 558,197 to 558,889 sfmC b0532: sfmD - predicted outer membrane export usher protein (NCBI), at 558,920 to 561,523 sfmD Position (kb) 557 558Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 557.005 kb on + strandat 557.074 kb on + strandat 557.074 kb on + strandat 557.081 kb on + strandat 557.086 kb on + strandat 557.090 kb on + strandat 557.092 kb on - strandat 557.092 kb on - strandat 557.094 kb on + strandat 557.125 kb on + strandat 557.125 kb on + strandat 557.171 kb on + strandat 557.226 kb on + strandat 557.584 kb on + strand, within sfmAat 557.588 kb on + strand, within sfmAat 557.707 kb on + strand, within sfmAat 557.767 kb on + strand, within sfmAat 557.786 kb on + strand, within sfmAat 557.824 kb on + strand, within sfmAat 557.838 kb on + strand, within sfmAat 557.845 kb on + strand, within sfmAat 557.856 kb on + strand, within sfmAat 557.870 kb on + strand, within sfmAat 557.900 kb on + strand, within sfmAat 557.900 kb on + strand, within sfmAat 557.902 kb on + strand, within sfmAat 557.902 kb on + strand, within sfmAat 557.914 kb on + strand, within sfmAat 557.916 kb on + strand, within sfmAat 557.916 kb on + strand, within sfmAat 557.925 kb on + strandat 557.925 kb on + strandat 557.928 kb on + strandat 557.933 kb on + strandat 557.938 kb on + strandat 557.940 kb on + strandat 557.957 kb on + strandat 557.957 kb on + strandat 558.080 kb on + strandat 558.084 kb on + strandat 558.274 kb on - strand, within sfmCat 558.320 kb on - strand, within sfmCat 558.320 kb on - strand, within sfmCat 558.332 kb on - strand, within sfmCat 558.334 kb on + strand, within sfmCat 558.383 kb on + strand, within sfmCat 558.404 kb on - strand, within sfmCat 558.425 kb on - strand, within sfmCat 558.439 kb on - strand, within sfmCat 558.480 kb on - strand, within sfmCat 558.480 kb on - strand, within sfmCat 558.480 kb on - strand, within sfmCat 558.488 kb on - strand, within sfmCat 558.501 kb on - strand, within sfmCat 558.517 kb on + strand, within sfmCat 558.539 kb on + strand, within sfmCat 558.556 kb on - strand, within sfmCat 558.650 kb on - strand, within sfmCat 558.659 kb on - strand, within sfmCat 558.693 kb on - strand, within sfmCat 558.695 kb on - strand, within sfmCat 558.695 kb on - strand, within sfmCat 558.695 kb on - strand, within sfmCat 558.719 kb on - strand, within sfmCat 558.719 kb on - strand, within sfmCat 558.722 kb on + strand, within sfmCat 558.722 kb on + strand, within sfmCat 558.733 kb on - strand, within sfmCat 558.735 kb on - strand, within sfmCat 558.740 kb on - strand, within sfmCat 558.740 kb on - strand, within sfmCat 558.755 kb on - strand, within sfmCat 558.763 kb on + strand, within sfmCat 558.763 kb on + strand, within sfmCat 558.763 kb on + strand, within sfmCat 558.771 kb on - strand, within sfmCat 558.771 kb on - strand, within sfmCat 558.771 kb on - strand, within sfmCat 558.774 kb on + strand, within sfmCat 558.775 kb on + strand, within sfmCat 558.810 kb on - strand, within sfmCat 558.812 kb on + strand, within sfmCat 558.812 kb on + strand, within sfmCat 558.812 kb on + strand, within sfmCat 558.812 kb on + strand, within sfmCat 558.820 kb on - strandat 558.852 kb on + strandat 558.852 kb on + strandat 558.855 kb on + strandat 558.906 kb on + strandat 558.939 kb on - strandat 558.955 kb on + strandat 558.960 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Isobutyl alcohol 1.25 vol%
remove
557,005 + +0.0
557,074 + +0.3
557,074 + -0.1
557,081 + +0.2
557,086 + -0.3
557,090 + -0.3
557,092 - +1.5
557,092 - +1.5
557,094 + +0.2
557,125 + +0.7
557,125 + -0.0
557,171 + -0.1
557,226 + +0.6
557,584 + sfmA b0530 0.27 +0.6
557,588 + sfmA b0530 0.28 +1.5
557,707 + sfmA b0530 0.50 +1.5
557,767 + sfmA b0530 0.61 +1.5
557,786 + sfmA b0530 0.65 +0.5
557,824 + sfmA b0530 0.72 +0.5
557,838 + sfmA b0530 0.74 +1.5
557,845 + sfmA b0530 0.76 +1.5
557,856 + sfmA b0530 0.78 +1.5
557,870 + sfmA b0530 0.80 +4.1
557,900 + sfmA b0530 0.86 +3.1
557,900 + sfmA b0530 0.86 +1.9
557,902 + sfmA b0530 0.86 +1.5
557,902 + sfmA b0530 0.86 +0.1
557,914 + sfmA b0530 0.88 +0.7
557,916 + sfmA b0530 0.89 -0.7
557,916 + sfmA b0530 0.89 +0.7
557,925 + +0.6
557,925 + -3.0
557,928 + -0.6
557,933 + -0.7
557,938 + -0.7
557,940 + -0.1
557,957 + -0.1
557,957 + -0.9
558,080 + +1.5
558,084 + +1.5
558,274 - sfmC b0531 0.11 +1.5
558,320 - sfmC b0531 0.18 +1.5
558,320 - sfmC b0531 0.18 +0.5
558,332 - sfmC b0531 0.19 +1.5
558,334 + sfmC b0531 0.20 +1.5
558,383 + sfmC b0531 0.27 -0.5
558,404 - sfmC b0531 0.30 +1.5
558,425 - sfmC b0531 0.33 +1.5
558,439 - sfmC b0531 0.35 +1.5
558,480 - sfmC b0531 0.41 +0.7
558,480 - sfmC b0531 0.41 +0.2
558,480 - sfmC b0531 0.41 +0.4
558,488 - sfmC b0531 0.42 +1.5
558,501 - sfmC b0531 0.44 +0.3
558,517 + sfmC b0531 0.46 +1.5
558,539 + sfmC b0531 0.49 +1.5
558,556 - sfmC b0531 0.52 +0.9
558,650 - sfmC b0531 0.65 -0.1
558,659 - sfmC b0531 0.67 +0.3
558,693 - sfmC b0531 0.72 -1.8
558,695 - sfmC b0531 0.72 +1.5
558,695 - sfmC b0531 0.72 +1.5
558,695 - sfmC b0531 0.72 +1.5
558,719 - sfmC b0531 0.75 +0.7
558,719 - sfmC b0531 0.75 +1.2
558,722 + sfmC b0531 0.76 +0.5
558,722 + sfmC b0531 0.76 -0.8
558,733 - sfmC b0531 0.77 -0.7
558,735 - sfmC b0531 0.78 -0.7
558,740 - sfmC b0531 0.78 +1.5
558,740 - sfmC b0531 0.78 +0.3
558,755 - sfmC b0531 0.81 +0.1
558,763 + sfmC b0531 0.82 +1.5
558,763 + sfmC b0531 0.82 +1.5
558,763 + sfmC b0531 0.82 +1.5
558,771 - sfmC b0531 0.83 +1.5
558,771 - sfmC b0531 0.83 -0.5
558,771 - sfmC b0531 0.83 +1.5
558,774 + sfmC b0531 0.83 +1.5
558,775 + sfmC b0531 0.83 +1.5
558,810 - sfmC b0531 0.88 -1.7
558,812 + sfmC b0531 0.89 +1.5
558,812 + sfmC b0531 0.89 +1.5
558,812 + sfmC b0531 0.89 +1.5
558,812 + sfmC b0531 0.89 +0.5
558,820 - +0.2
558,852 + -0.1
558,852 + +2.5
558,855 + +1.5
558,906 + +1.5
558,939 - +1.5
558,955 + +1.5
558,960 - +0.5

Or see this region's nucleotide sequence