Experiment: M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4125 and PP_4126 are separated by 11 nucleotides PP_4126 and PP_4127 are separated by 10 nucleotides PP_4127 and PP_4128 are separated by 3 nucleotides PP_4128 and PP_4129 overlap by 4 nucleotides
PP_4125: PP_4125 - NADH-quinone oxidoreductase subunit H, at 4,663,848 to 4,664,855
_4125
PP_4126: PP_4126 - NADH-quinone oxidoreductase subunit I, at 4,664,867 to 4,665,415
_4126
PP_4127: PP_4127 - NADH-quinone oxidoreductase subunit J, at 4,665,426 to 4,665,926
_4127
PP_4128: PP_4128 - NADH-quinone oxidoreductase subunit K, at 4,665,930 to 4,666,238
_4128
PP_4129: PP_4129 - NADH:ubiquinone oxidoreductase, membrane subunit L, at 4,666,235 to 4,668,088
_4129
Position (kb)
4664
4665
4666 Strain fitness (log2 ratio)
-1
0
1 at 4664.313 kb on + strand at 4664.314 kb on - strand, within PP_4125 at 4664.574 kb on + strand, within PP_4125 at 4664.574 kb on + strand, within PP_4125 at 4664.574 kb on + strand, within PP_4125 at 4664.575 kb on - strand, within PP_4125 at 4664.826 kb on + strand at 4664.826 kb on + strand at 4664.826 kb on + strand at 4664.876 kb on + strand at 4664.876 kb on + strand at 4664.876 kb on + strand at 4664.877 kb on - strand at 4664.893 kb on + strand at 4664.894 kb on - strand at 4665.054 kb on + strand, within PP_4126 at 4665.266 kb on + strand, within PP_4126 at 4665.266 kb on + strand, within PP_4126 at 4665.323 kb on + strand, within PP_4126 at 4665.323 kb on + strand at 4665.324 kb on - strand, within PP_4126 at 4665.324 kb on - strand, within PP_4126 at 4665.441 kb on + strand at 4665.441 kb on + strand at 4665.484 kb on + strand, within PP_4127 at 4665.750 kb on + strand, within PP_4127 at 4665.750 kb on + strand, within PP_4127 at 4665.751 kb on - strand, within PP_4127 at 4665.924 kb on + strand at 4666.373 kb on + strand at 4666.373 kb on + strand at 4666.374 kb on - strand at 4666.374 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB remove 4,664,313 + -0.5 4,664,314 - PP_4125 0.46 -0.6 4,664,574 + PP_4125 0.72 -0.9 4,664,574 + PP_4125 0.72 +0.8 4,664,574 + PP_4125 0.72 +0.1 4,664,575 - PP_4125 0.72 -1.4 4,664,826 + +0.2 4,664,826 + -0.6 4,664,826 + -1.3 4,664,876 + +0.2 4,664,876 + +0.8 4,664,876 + +0.8 4,664,877 - +0.5 4,664,893 + +0.3 4,664,894 - -0.5 4,665,054 + PP_4126 0.34 -0.4 4,665,266 + PP_4126 0.73 -0.0 4,665,266 + PP_4126 0.73 -0.8 4,665,323 + PP_4126 0.83 +0.3 4,665,323 + +0.9 4,665,324 - PP_4126 0.83 +0.3 4,665,324 - PP_4126 0.83 +0.1 4,665,441 + -0.2 4,665,441 + -1.5 4,665,484 + PP_4127 0.12 -0.8 4,665,750 + PP_4127 0.65 -0.7 4,665,750 + PP_4127 0.65 -1.6 4,665,751 - PP_4127 0.65 +0.5 4,665,924 + -0.9 4,666,373 + -1.2 4,666,373 + +0.3 4,666,374 - +0.3 4,666,374 - -1.1
Or see this region's nucleotide sequence