Experiment: M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3409 and PP_3410 are separated by 34 nucleotides PP_3410 and PP_3411 are separated by 66 nucleotides PP_3411 and PP_3412 are separated by 221 nucleotides PP_3412 and PP_3413 are separated by 13 nucleotides
PP_3409: PP_3409 - putative Cobalamin biosynthesis protein cobE, at 3,858,743 to 3,859,132
_3409
PP_3410: PP_3410 - Precorrin-4 C(11)-methyltransferase, at 3,859,167 to 3,859,919
_3410
PP_3411: PP_3411 - conserved protein of unknown function, at 3,859,986 to 3,860,228
_3411
PP_3412: PP_3412 - DNA-binding response regulator, LuxR family, at 3,860,450 to 3,861,064
_3412
PP_3413: PP_3413 - Sensor histidine kinase/response regulator, at 3,861,078 to 3,864,359
_3413
Position (kb)
3859
3860
3861 Strain fitness (log2 ratio)
-1
0
1 at 3859.098 kb on + strand at 3859.176 kb on + strand at 3859.176 kb on + strand at 3859.177 kb on - strand at 3859.177 kb on - strand at 3859.177 kb on - strand at 3859.423 kb on - strand, within PP_3410 at 3859.835 kb on - strand, within PP_3410 at 3859.853 kb on + strand at 3859.853 kb on + strand at 3859.878 kb on + strand at 3859.878 kb on + strand at 3859.878 kb on + strand at 3859.878 kb on + strand at 3859.879 kb on - strand at 3859.996 kb on + strand at 3860.140 kb on + strand, within PP_3411 at 3860.200 kb on + strand, within PP_3411 at 3860.288 kb on - strand at 3860.303 kb on + strand at 3860.303 kb on + strand at 3860.304 kb on - strand at 3860.304 kb on - strand at 3860.304 kb on - strand at 3860.304 kb on - strand at 3860.304 kb on - strand at 3860.304 kb on - strand at 3860.328 kb on + strand at 3860.328 kb on + strand at 3860.329 kb on - strand at 3860.363 kb on - strand at 3860.399 kb on + strand at 3860.399 kb on + strand at 3860.400 kb on - strand at 3860.434 kb on + strand at 3860.482 kb on + strand at 3860.482 kb on + strand at 3860.483 kb on - strand at 3860.505 kb on + strand at 3860.572 kb on - strand, within PP_3412 at 3860.595 kb on + strand, within PP_3412 at 3860.596 kb on - strand, within PP_3412 at 3860.711 kb on + strand, within PP_3412 at 3860.711 kb on + strand, within PP_3412 at 3860.711 kb on + strand, within PP_3412 at 3860.711 kb on + strand, within PP_3412 at 3860.711 kb on + strand, within PP_3412 at 3860.712 kb on - strand, within PP_3412 at 3860.740 kb on - strand, within PP_3412 at 3860.810 kb on + strand, within PP_3412 at 3860.811 kb on - strand, within PP_3412 at 3860.981 kb on + strand, within PP_3412 at 3861.059 kb on + strand at 3861.060 kb on - strand at 3861.060 kb on - strand at 3861.060 kb on - strand at 3861.203 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB remove 3,859,098 + -0.3 3,859,176 + -0.5 3,859,176 + -0.5 3,859,177 - +0.0 3,859,177 - -0.6 3,859,177 - +0.4 3,859,423 - PP_3410 0.34 +0.5 3,859,835 - PP_3410 0.89 +0.3 3,859,853 + -0.9 3,859,853 + -0.9 3,859,878 + +0.4 3,859,878 + -0.4 3,859,878 + +0.6 3,859,878 + +0.3 3,859,879 - +0.2 3,859,996 + +1.2 3,860,140 + PP_3411 0.63 -0.2 3,860,200 + PP_3411 0.88 -1.7 3,860,288 - +0.4 3,860,303 + +0.1 3,860,303 + +1.7 3,860,304 - -0.0 3,860,304 - +0.4 3,860,304 - +0.4 3,860,304 - +0.6 3,860,304 - -0.4 3,860,304 - -0.0 3,860,328 + +0.4 3,860,328 + -0.2 3,860,329 - +0.5 3,860,363 - -0.2 3,860,399 + +0.1 3,860,399 + -1.3 3,860,400 - -0.7 3,860,434 + +0.3 3,860,482 + +0.2 3,860,482 + +0.1 3,860,483 - -0.8 3,860,505 + +0.7 3,860,572 - PP_3412 0.20 +0.2 3,860,595 + PP_3412 0.24 +0.5 3,860,596 - PP_3412 0.24 -0.2 3,860,711 + PP_3412 0.42 +0.2 3,860,711 + PP_3412 0.42 +0.8 3,860,711 + PP_3412 0.42 +0.2 3,860,711 + PP_3412 0.42 +0.4 3,860,711 + PP_3412 0.42 -1.1 3,860,712 - PP_3412 0.43 -1.1 3,860,740 - PP_3412 0.47 +0.1 3,860,810 + PP_3412 0.59 -0.5 3,860,811 - PP_3412 0.59 +0.5 3,860,981 + PP_3412 0.86 +0.2 3,861,059 + -1.1 3,861,060 - -1.1 3,861,060 - -0.0 3,861,060 - -0.2 3,861,203 - +0.1
Or see this region's nucleotide sequence