Experiment: M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1588 and PP_1589 are separated by 30 nucleotides PP_1589 and PP_1590 are separated by 41 nucleotides
PP_1588: PP_1588 - N-succinyl-L,L-diaminopimelate aminotransferase alternative, at 1,780,147 to 1,781,343
_1588
PP_1589: PP_1589 - uridylyltransferase, at 1,781,374 to 1,784,076
_1589
PP_1590: PP_1590 - methionine aminopeptidase, at 1,784,118 to 1,784,900
_1590
Position (kb)
1781
1782
1783
1784
1785 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1780.422 kb on - strand, within PP_1588 at 1780.454 kb on - strand, within PP_1588 at 1780.454 kb on - strand, within PP_1588 at 1780.523 kb on + strand, within PP_1588 at 1780.523 kb on + strand, within PP_1588 at 1780.706 kb on + strand, within PP_1588 at 1780.706 kb on + strand, within PP_1588 at 1780.706 kb on + strand, within PP_1588 at 1780.707 kb on - strand, within PP_1588 at 1780.807 kb on - strand, within PP_1588 at 1780.913 kb on + strand, within PP_1588 at 1780.914 kb on - strand, within PP_1588 at 1780.914 kb on - strand, within PP_1588 at 1780.952 kb on + strand, within PP_1588 at 1780.953 kb on - strand, within PP_1588 at 1780.953 kb on - strand, within PP_1588 at 1780.961 kb on + strand, within PP_1588 at 1780.962 kb on - strand, within PP_1588 at 1782.245 kb on + strand, within PP_1589 at 1782.245 kb on + strand, within PP_1589 at 1782.246 kb on - strand, within PP_1589 at 1782.246 kb on - strand, within PP_1589 at 1782.322 kb on + strand, within PP_1589 at 1782.322 kb on + strand, within PP_1589 at 1782.322 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.326 kb on + strand, within PP_1589 at 1782.327 kb on - strand, within PP_1589 at 1782.327 kb on - strand, within PP_1589 at 1782.327 kb on - strand, within PP_1589 at 1782.327 kb on - strand, within PP_1589 at 1782.327 kb on - strand, within PP_1589 at 1782.327 kb on - strand, within PP_1589 at 1782.332 kb on + strand, within PP_1589 at 1782.332 kb on + strand, within PP_1589 at 1782.333 kb on - strand, within PP_1589 at 1782.560 kb on + strand, within PP_1589 at 1782.561 kb on - strand, within PP_1589 at 1782.576 kb on - strand, within PP_1589 at 1782.647 kb on + strand, within PP_1589 at 1782.647 kb on + strand, within PP_1589 at 1782.648 kb on - strand, within PP_1589 at 1782.648 kb on - strand, within PP_1589 at 1782.685 kb on - strand, within PP_1589 at 1782.685 kb on - strand, within PP_1589 at 1782.699 kb on + strand, within PP_1589 at 1782.699 kb on + strand, within PP_1589 at 1782.700 kb on - strand, within PP_1589 at 1782.700 kb on - strand at 1782.700 kb on - strand, within PP_1589 at 1782.701 kb on + strand, within PP_1589 at 1782.701 kb on + strand, within PP_1589 at 1782.701 kb on + strand, within PP_1589 at 1782.702 kb on - strand, within PP_1589 at 1782.702 kb on - strand, within PP_1589 at 1782.846 kb on + strand, within PP_1589 at 1782.931 kb on + strand at 1783.020 kb on - strand, within PP_1589 at 1784.957 kb on - strand at 1784.957 kb on - strand at 1785.007 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 minimal media_noCarbon with D-Glucose 10 mM; 30C; then outgrowth in LB remove 1,780,422 - PP_1588 0.23 -0.1 1,780,454 - PP_1588 0.26 -0.3 1,780,454 - PP_1588 0.26 -0.4 1,780,523 + PP_1588 0.31 -1.3 1,780,523 + PP_1588 0.31 -0.3 1,780,706 + PP_1588 0.47 +0.1 1,780,706 + PP_1588 0.47 +0.2 1,780,706 + PP_1588 0.47 +0.8 1,780,707 - PP_1588 0.47 -0.1 1,780,807 - PP_1588 0.55 -2.1 1,780,913 + PP_1588 0.64 -0.3 1,780,914 - PP_1588 0.64 -0.7 1,780,914 - PP_1588 0.64 +1.3 1,780,952 + PP_1588 0.67 +0.5 1,780,953 - PP_1588 0.67 +0.7 1,780,953 - PP_1588 0.67 -0.1 1,780,961 + PP_1588 0.68 -0.5 1,780,962 - PP_1588 0.68 +0.5 1,782,245 + PP_1589 0.32 +0.5 1,782,245 + PP_1589 0.32 +0.2 1,782,246 - PP_1589 0.32 -1.7 1,782,246 - PP_1589 0.32 -1.2 1,782,322 + PP_1589 0.35 -1.6 1,782,322 + PP_1589 0.35 -0.5 1,782,322 + PP_1589 0.35 -0.2 1,782,326 + PP_1589 0.35 -0.7 1,782,326 + PP_1589 0.35 -0.6 1,782,326 + PP_1589 0.35 -1.4 1,782,326 + PP_1589 0.35 -0.4 1,782,326 + PP_1589 0.35 -0.2 1,782,326 + PP_1589 0.35 -1.4 1,782,326 + PP_1589 0.35 -1.1 1,782,327 - PP_1589 0.35 +0.5 1,782,327 - PP_1589 0.35 +0.1 1,782,327 - PP_1589 0.35 -0.5 1,782,327 - PP_1589 0.35 -0.4 1,782,327 - PP_1589 0.35 -1.0 1,782,327 - PP_1589 0.35 -0.6 1,782,332 + PP_1589 0.35 -1.3 1,782,332 + PP_1589 0.35 -1.2 1,782,333 - PP_1589 0.35 -0.2 1,782,560 + PP_1589 0.44 -0.2 1,782,561 - PP_1589 0.44 -1.6 1,782,576 - PP_1589 0.44 -0.8 1,782,647 + PP_1589 0.47 +0.0 1,782,647 + PP_1589 0.47 -0.3 1,782,648 - PP_1589 0.47 -3.0 1,782,648 - PP_1589 0.47 -0.3 1,782,685 - PP_1589 0.49 +0.0 1,782,685 - PP_1589 0.49 -0.7 1,782,699 + PP_1589 0.49 -2.3 1,782,699 + PP_1589 0.49 -1.1 1,782,700 - PP_1589 0.49 -0.4 1,782,700 - +0.6 1,782,700 - PP_1589 0.49 -0.1 1,782,701 + PP_1589 0.49 -1.7 1,782,701 + PP_1589 0.49 -0.1 1,782,701 + PP_1589 0.49 -2.9 1,782,702 - PP_1589 0.49 -0.5 1,782,702 - PP_1589 0.49 -3.0 1,782,846 + PP_1589 0.54 -1.0 1,782,931 + -0.4 1,783,020 - PP_1589 0.61 +0.1 1,784,957 - -0.3 1,784,957 - -1.0 1,785,007 + +0.1
Or see this region's nucleotide sequence