Experiment: M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5188 and PP_5189 are separated by 126 nucleotides PP_5189 and PP_5190 are separated by 107 nucleotides PP_5190 and PP_5191 are separated by 88 nucleotides PP_5191 and PP_5192 are separated by 65 nucleotides
PP_5188: PP_5188 - putative transcriptional regulator (AsnC/Lrp family), at 5,918,405 to 5,918,875
_5188
PP_5189: PP_5189 - conserved protein of unknown function, at 5,919,002 to 5,919,307
_5189
PP_5190: PP_5190 - Type II secretion system protein, at 5,919,415 to 5,921,088
_5190
PP_5191: PP_5191 - conserved exported protein of unknown function, at 5,921,177 to 5,921,491
_5191
PP_5192: PP_5192 - glycine dehydrogenase, at 5,921,557 to 5,924,430
_5192
Position (kb)
5919
5920
5921
5922 Strain fitness (log2 ratio)
-1
0
1 at 5918.425 kb on + strand at 5918.518 kb on - strand, within PP_5188 at 5918.577 kb on + strand, within PP_5188 at 5918.577 kb on + strand, within PP_5188 at 5918.577 kb on + strand, within PP_5188 at 5918.577 kb on + strand, within PP_5188 at 5918.578 kb on - strand, within PP_5188 at 5918.578 kb on - strand, within PP_5188 at 5918.578 kb on - strand, within PP_5188 at 5918.578 kb on - strand, within PP_5188 at 5918.578 kb on - strand, within PP_5188 at 5918.578 kb on - strand, within PP_5188 at 5918.622 kb on + strand, within PP_5188 at 5918.877 kb on - strand at 5918.906 kb on + strand at 5918.998 kb on + strand at 5918.998 kb on + strand at 5918.998 kb on + strand at 5918.998 kb on + strand at 5918.998 kb on + strand at 5918.998 kb on + strand at 5918.999 kb on - strand at 5919.001 kb on + strand at 5919.653 kb on - strand, within PP_5190 at 5919.653 kb on - strand, within PP_5190 at 5919.653 kb on - strand, within PP_5190 at 5919.845 kb on + strand, within PP_5190 at 5920.017 kb on + strand, within PP_5190 at 5920.039 kb on + strand, within PP_5190 at 5920.040 kb on - strand, within PP_5190 at 5920.040 kb on - strand, within PP_5190 at 5920.135 kb on + strand, within PP_5190 at 5920.135 kb on + strand, within PP_5190 at 5920.136 kb on - strand, within PP_5190 at 5920.723 kb on - strand, within PP_5190 at 5920.911 kb on - strand, within PP_5190 at 5921.103 kb on + strand at 5921.245 kb on + strand, within PP_5191 at 5921.278 kb on + strand, within PP_5191 at 5921.295 kb on - strand, within PP_5191 at 5921.322 kb on + strand, within PP_5191 at 5921.525 kb on + strand at 5921.565 kb on - strand at 5921.610 kb on - strand at 5921.621 kb on - strand at 5921.642 kb on + strand at 5921.643 kb on - strand at 5921.643 kb on - strand at 5921.643 kb on - strand at 5921.643 kb on - strand at 5921.692 kb on - strand at 5921.693 kb on + strand at 5921.693 kb on + strand at 5921.694 kb on - strand at 5921.694 kb on - strand at 5921.694 kb on - strand at 5921.730 kb on + strand at 5921.731 kb on - strand at 5921.782 kb on + strand at 5921.782 kb on + strand at 5921.782 kb on + strand at 5921.782 kb on + strand at 5921.783 kb on - strand at 5921.783 kb on - strand at 5921.783 kb on - strand at 5921.918 kb on + strand, within PP_5192 at 5921.918 kb on + strand, within PP_5192 at 5921.918 kb on + strand, within PP_5192 at 5921.919 kb on - strand, within PP_5192 at 5922.026 kb on + strand, within PP_5192 at 5922.066 kb on - strand, within PP_5192 at 5922.066 kb on - strand, within PP_5192 at 5922.066 kb on - strand, within PP_5192
Per-strain Table
Position Strand Gene LocusTag Fraction M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB remove 5,918,425 + +0.2 5,918,518 - PP_5188 0.24 +0.1 5,918,577 + PP_5188 0.37 -0.2 5,918,577 + PP_5188 0.37 +0.4 5,918,577 + PP_5188 0.37 +0.7 5,918,577 + PP_5188 0.37 +0.4 5,918,578 - PP_5188 0.37 +0.2 5,918,578 - PP_5188 0.37 -0.1 5,918,578 - PP_5188 0.37 -0.0 5,918,578 - PP_5188 0.37 -0.1 5,918,578 - PP_5188 0.37 +0.1 5,918,578 - PP_5188 0.37 -0.3 5,918,622 + PP_5188 0.46 +0.4 5,918,877 - +0.2 5,918,906 + +0.3 5,918,998 + -0.2 5,918,998 + +1.2 5,918,998 + -0.1 5,918,998 + -0.5 5,918,998 + -0.2 5,918,998 + +0.2 5,918,999 - -0.1 5,919,001 + -0.4 5,919,653 - PP_5190 0.14 +0.7 5,919,653 - PP_5190 0.14 +0.6 5,919,653 - PP_5190 0.14 +0.9 5,919,845 + PP_5190 0.26 -0.5 5,920,017 + PP_5190 0.36 +0.3 5,920,039 + PP_5190 0.37 -0.3 5,920,040 - PP_5190 0.37 +1.3 5,920,040 - PP_5190 0.37 +0.1 5,920,135 + PP_5190 0.43 +0.6 5,920,135 + PP_5190 0.43 +0.2 5,920,136 - PP_5190 0.43 +0.6 5,920,723 - PP_5190 0.78 -0.1 5,920,911 - PP_5190 0.89 -0.5 5,921,103 + -0.7 5,921,245 + PP_5191 0.22 -0.3 5,921,278 + PP_5191 0.32 +0.4 5,921,295 - PP_5191 0.37 +0.3 5,921,322 + PP_5191 0.46 -0.3 5,921,525 + -1.5 5,921,565 - -0.9 5,921,610 - -0.4 5,921,621 - +0.2 5,921,642 + -0.1 5,921,643 - +0.1 5,921,643 - -0.0 5,921,643 - +0.0 5,921,643 - -0.2 5,921,692 - +0.2 5,921,693 + +0.1 5,921,693 + +0.0 5,921,694 - -0.0 5,921,694 - -0.4 5,921,694 - -0.6 5,921,730 + -0.2 5,921,731 - -0.4 5,921,782 + +0.7 5,921,782 + -0.6 5,921,782 + +0.6 5,921,782 + +0.4 5,921,783 - +0.5 5,921,783 - +0.8 5,921,783 - +0.1 5,921,918 + PP_5192 0.13 -0.8 5,921,918 + PP_5192 0.13 -0.8 5,921,918 + PP_5192 0.13 +1.2 5,921,919 - PP_5192 0.13 +0.1 5,922,026 + PP_5192 0.16 +0.0 5,922,066 - PP_5192 0.18 +0.4 5,922,066 - PP_5192 0.18 -0.2 5,922,066 - PP_5192 0.18 +1.4
Or see this region's nucleotide sequence