Experiment: M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1508 and PP_1509 are separated by 3 nucleotides PP_1509 and PP_1510 are separated by 28 nucleotides PP_1510 and PP_1511 overlap by 1 nucleotides PP_1511 and PP_1512 are separated by 2 nucleotides
PP_1508: PP_1508 - conserved protein of unknown function, at 1,713,833 to 1,714,153
_1508
PP_1509: PP_1509 - conserved protein of unknown function, at 1,714,157 to 1,715,026
_1509
PP_1510: PP_1510 - putative Carboxyvinyl-carboxyphosphonate phosphorylmutase, at 1,715,055 to 1,715,885
_1510
PP_1511: PP_1511 - conserved protein of unknown function, at 1,715,885 to 1,716,574
_1511
PP_1512: PP_1512 - conserved protein of unknown function, at 1,716,577 to 1,717,197
_1512
Position (kb)
1715
1716 Strain fitness (log2 ratio)
-1
0
1 at 1714.088 kb on + strand, within PP_1508 at 1714.088 kb on + strand, within PP_1508 at 1714.089 kb on - strand, within PP_1508 at 1714.089 kb on - strand, within PP_1508 at 1714.145 kb on + strand at 1714.178 kb on + strand at 1714.223 kb on + strand at 1714.223 kb on + strand at 1714.224 kb on - strand at 1714.224 kb on - strand at 1714.269 kb on - strand, within PP_1509 at 1714.310 kb on + strand, within PP_1509 at 1714.311 kb on - strand, within PP_1509 at 1714.480 kb on - strand, within PP_1509 at 1714.488 kb on - strand, within PP_1509 at 1714.488 kb on - strand, within PP_1509 at 1714.660 kb on + strand, within PP_1509 at 1714.660 kb on + strand, within PP_1509 at 1714.661 kb on - strand, within PP_1509 at 1714.661 kb on - strand, within PP_1509 at 1714.694 kb on - strand, within PP_1509 at 1714.828 kb on + strand, within PP_1509 at 1714.880 kb on + strand, within PP_1509 at 1714.880 kb on + strand, within PP_1509 at 1714.880 kb on + strand, within PP_1509 at 1714.880 kb on + strand, within PP_1509 at 1714.881 kb on - strand, within PP_1509 at 1714.881 kb on - strand, within PP_1509 at 1714.881 kb on - strand, within PP_1509 at 1714.881 kb on - strand, within PP_1509 at 1714.881 kb on - strand, within PP_1509 at 1714.882 kb on + strand, within PP_1509 at 1714.882 kb on + strand, within PP_1509 at 1714.883 kb on - strand, within PP_1509 at 1714.883 kb on - strand, within PP_1509 at 1715.020 kb on - strand at 1715.095 kb on + strand at 1715.187 kb on - strand, within PP_1510 at 1715.433 kb on + strand, within PP_1510 at 1715.433 kb on + strand, within PP_1510 at 1715.434 kb on + strand, within PP_1510 at 1715.434 kb on - strand, within PP_1510 at 1715.580 kb on + strand, within PP_1510 at 1715.581 kb on - strand, within PP_1510 at 1715.581 kb on - strand, within PP_1510 at 1715.608 kb on - strand, within PP_1510 at 1715.790 kb on + strand, within PP_1510 at 1715.791 kb on - strand, within PP_1510 at 1715.994 kb on + strand, within PP_1511 at 1715.994 kb on + strand, within PP_1511 at 1715.995 kb on - strand, within PP_1511 at 1716.040 kb on - strand, within PP_1511 at 1716.144 kb on + strand, within PP_1511 at 1716.145 kb on - strand, within PP_1511 at 1716.215 kb on + strand, within PP_1511 at 1716.320 kb on + strand, within PP_1511 at 1716.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 minimal media_noCarbon with Trisodium citrate 10 mM; 30C; then outgrowth in LB remove 1,714,088 + PP_1508 0.79 +0.3 1,714,088 + PP_1508 0.79 +0.0 1,714,089 - PP_1508 0.80 +0.4 1,714,089 - PP_1508 0.80 +0.7 1,714,145 + +0.3 1,714,178 + -0.8 1,714,223 + -0.8 1,714,223 + +0.0 1,714,224 - +0.3 1,714,224 - -0.2 1,714,269 - PP_1509 0.13 +0.7 1,714,310 + PP_1509 0.18 +0.7 1,714,311 - PP_1509 0.18 +0.2 1,714,480 - PP_1509 0.37 -0.0 1,714,488 - PP_1509 0.38 -0.9 1,714,488 - PP_1509 0.38 +0.8 1,714,660 + PP_1509 0.58 +0.4 1,714,660 + PP_1509 0.58 +0.0 1,714,661 - PP_1509 0.58 -0.4 1,714,661 - PP_1509 0.58 +1.2 1,714,694 - PP_1509 0.62 +0.1 1,714,828 + PP_1509 0.77 -0.2 1,714,880 + PP_1509 0.83 +0.7 1,714,880 + PP_1509 0.83 +0.7 1,714,880 + PP_1509 0.83 -0.0 1,714,880 + PP_1509 0.83 +0.9 1,714,881 - PP_1509 0.83 +0.5 1,714,881 - PP_1509 0.83 -0.5 1,714,881 - PP_1509 0.83 +0.2 1,714,881 - PP_1509 0.83 -0.2 1,714,881 - PP_1509 0.83 -0.5 1,714,882 + PP_1509 0.83 -0.3 1,714,882 + PP_1509 0.83 -0.1 1,714,883 - PP_1509 0.83 +0.4 1,714,883 - PP_1509 0.83 +0.0 1,715,020 - -0.2 1,715,095 + -0.1 1,715,187 - PP_1510 0.16 +0.5 1,715,433 + PP_1510 0.45 +0.3 1,715,433 + PP_1510 0.45 -0.2 1,715,434 + PP_1510 0.46 +0.9 1,715,434 - PP_1510 0.46 +0.2 1,715,580 + PP_1510 0.63 +0.4 1,715,581 - PP_1510 0.63 +0.4 1,715,581 - PP_1510 0.63 +0.6 1,715,608 - PP_1510 0.67 -0.0 1,715,790 + PP_1510 0.88 -0.2 1,715,791 - PP_1510 0.89 -0.0 1,715,994 + PP_1511 0.16 -0.5 1,715,994 + PP_1511 0.16 -0.7 1,715,995 - PP_1511 0.16 +0.3 1,716,040 - PP_1511 0.22 -0.3 1,716,144 + PP_1511 0.38 +0.2 1,716,145 - PP_1511 0.38 -0.4 1,716,215 + PP_1511 0.48 +0.4 1,716,320 + PP_1511 0.63 -0.2 1,716,512 + +0.2
Or see this region's nucleotide sequence