Experiment: AgroPhage_F10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0380 and Atu0381 are separated by 47 nucleotides Atu0381 and Atu0382 are separated by 167 nucleotides Atu0382 and Atu0383 are separated by 12 nucleotides Atu0383 and Atu0384 overlap by 4 nucleotides
Atu0380: Atu0380 - acetylglutamate kinase, at 373,783 to 374,667
Atu0380
Atu0381: Atu0381 - hypothetical protein, at 374,715 to 374,855
Atu0381
Atu0382: Atu0382 - hypothetical protein, at 375,023 to 375,400
Atu0382
Atu0383: Atu0383 - GTP-binding protein, at 375,413 to 376,066
Atu0383
Atu0384: Atu0384 - preprotein translocase 60 kD membrane subunit, at 376,063 to 377,862
Atu0384
Position (kb) 375
376
377 Strain fitness (log2 ratio) -2
-1
0
1 at 374.721 kb on - strand at 374.767 kb on + strand, within Atu0381 at 374.767 kb on + strand, within Atu0381 at 374.768 kb on - strand, within Atu0381 at 374.921 kb on - strand at 374.944 kb on + strand at 374.988 kb on - strand at 375.238 kb on + strand, within Atu0382 at 375.249 kb on + strand, within Atu0382 at 375.252 kb on - strand, within Atu0382 at 375.257 kb on - strand, within Atu0382 at 375.372 kb on - strand at 375.382 kb on + strand at 375.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction AgroPhage_F10 remove 374,721 - +0.5 374,767 + Atu0381 0.37 +0.5 374,767 + Atu0381 0.37 -0.2 374,768 - Atu0381 0.38 +1.1 374,921 - -0.3 374,944 + -0.5 374,988 - -0.2 375,238 + Atu0382 0.57 -0.2 375,249 + Atu0382 0.60 -2.7 375,252 - Atu0382 0.61 +0.4 375,257 - Atu0382 0.62 -1.3 375,372 - -1.4 375,382 + -2.6 375,382 + +1.1
Or see this region's nucleotide sequence