Experiment: AgroPhage_F04
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1244 and Atu1245 are separated by 70 nucleotides Atu1245 and Atu1246 are separated by 131 nucleotides Atu1246 and Atu1247 are separated by 2 nucleotides
Atu1243: Atu1243 - streptomycin resistance protein, at 1,233,104 to 1,233,949
Atu1243
Atu1244: Atu1244 - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,233,946 to 1,234,878
Atu1244
Atu1245: Atu1245 - agmatinase, at 1,234,949 to 1,235,902
Atu1245
Atu1246: Atu1246 - 30S ribosomal protein S9, at 1,236,034 to 1,236,501
Atu1246
Atu1247: Atu1247 - 50S ribosomal protein L13, at 1,236,504 to 1,236,968
Atu1247
Position (kb)
1234
1235
1236 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1234.989 kb on - strand at 1234.994 kb on - strand at 1234.996 kb on + strand at 1235.002 kb on - strand at 1235.094 kb on - strand, within Atu1245 at 1235.109 kb on - strand, within Atu1245 at 1235.211 kb on + strand, within Atu1245 at 1235.305 kb on + strand, within Atu1245 at 1235.374 kb on + strand, within Atu1245 at 1235.428 kb on - strand, within Atu1245 at 1235.503 kb on + strand, within Atu1245 at 1235.577 kb on - strand, within Atu1245 at 1235.683 kb on + strand, within Atu1245 at 1235.683 kb on - strand, within Atu1245 at 1235.781 kb on + strand, within Atu1245 at 1235.789 kb on - strand, within Atu1245 at 1235.816 kb on + strand at 1235.833 kb on + strand at 1235.875 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction AgroPhage_F04 remove 1,234,989 - -1.4 1,234,994 - -1.0 1,234,996 + +1.5 1,235,002 - +0.5 1,235,094 - Atu1245 0.15 -2.1 1,235,109 - Atu1245 0.17 -0.4 1,235,211 + Atu1245 0.27 +2.0 1,235,305 + Atu1245 0.37 +0.1 1,235,374 + Atu1245 0.45 -3.4 1,235,428 - Atu1245 0.50 +0.9 1,235,503 + Atu1245 0.58 +1.5 1,235,577 - Atu1245 0.66 +0.1 1,235,683 + Atu1245 0.77 +0.7 1,235,683 - Atu1245 0.77 -0.3 1,235,781 + Atu1245 0.87 -0.0 1,235,789 - Atu1245 0.88 -0.0 1,235,816 + -0.1 1,235,833 + -0.8 1,235,875 + +0.2
Or see this region's nucleotide sequence