Experiment: AgroPhage_F04
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0122 and Atu0123 are separated by 306 nucleotides Atu0123 and Atu0124 are separated by 221 nucleotides Atu0124 and Atu0125 are separated by 72 nucleotides Atu0125 and Atu0126 are separated by 213 nucleotides
Atu0122: Atu0122 - DNAK Protein, at 126,205 to 128,106
Atu0122
Atu0123: Atu0123 - tRNA-Ser, at 128,413 to 128,502
Atu0123
Atu0124: Atu0124 - hypothetical protein, at 128,724 to 129,041
Atu0124
Atu0125: Atu0125 - peptide methionine sulfoxide reductase, at 129,114 to 129,764
Atu0125
Atu0126: Atu0126 - membrane lipoprotein, at 129,978 to 130,970
Atu0126
Position (kb)
128
129
130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 128.235 kb on + strand at 128.235 kb on + strand at 128.243 kb on - strand at 128.243 kb on - strand at 128.243 kb on - strand at 128.281 kb on - strand at 128.291 kb on - strand at 128.393 kb on - strand at 128.407 kb on + strand at 128.820 kb on + strand, within Atu0124 at 128.862 kb on - strand, within Atu0124 at 128.873 kb on - strand, within Atu0124 at 128.987 kb on - strand, within Atu0124 at 128.987 kb on - strand, within Atu0124 at 129.026 kb on - strand at 129.060 kb on - strand at 129.067 kb on + strand at 129.083 kb on - strand at 129.094 kb on - strand at 129.239 kb on + strand, within Atu0125 at 129.239 kb on + strand, within Atu0125 at 129.247 kb on - strand, within Atu0125 at 129.416 kb on + strand, within Atu0125 at 129.424 kb on - strand, within Atu0125 at 129.425 kb on + strand, within Atu0125 at 129.461 kb on + strand, within Atu0125 at 129.461 kb on + strand, within Atu0125 at 129.469 kb on - strand, within Atu0125 at 129.485 kb on + strand, within Atu0125 at 129.521 kb on + strand, within Atu0125 at 129.611 kb on + strand, within Atu0125 at 129.611 kb on + strand, within Atu0125 at 129.619 kb on - strand, within Atu0125 at 129.629 kb on - strand, within Atu0125 at 129.718 kb on - strand at 129.741 kb on + strand at 129.753 kb on + strand at 129.963 kb on + strand at 129.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction AgroPhage_F04 remove 128,235 + -0.1 128,235 + +0.2 128,243 - -1.8 128,243 - +0.7 128,243 - -0.3 128,281 - +0.2 128,291 - -1.7 128,393 - +1.0 128,407 + +0.0 128,820 + Atu0124 0.30 +0.9 128,862 - Atu0124 0.43 +1.1 128,873 - Atu0124 0.47 +2.5 128,987 - Atu0124 0.83 +1.0 128,987 - Atu0124 0.83 +0.5 129,026 - +0.5 129,060 - -1.6 129,067 + -1.2 129,083 - -1.9 129,094 - +0.0 129,239 + Atu0125 0.19 +0.3 129,239 + Atu0125 0.19 +1.0 129,247 - Atu0125 0.20 +1.1 129,416 + Atu0125 0.46 -1.0 129,424 - Atu0125 0.48 +0.9 129,425 + Atu0125 0.48 +0.5 129,461 + Atu0125 0.53 -0.6 129,461 + Atu0125 0.53 -0.2 129,469 - Atu0125 0.55 +0.1 129,485 + Atu0125 0.57 -0.3 129,521 + Atu0125 0.63 -1.3 129,611 + Atu0125 0.76 +0.1 129,611 + Atu0125 0.76 +0.9 129,619 - Atu0125 0.78 +0.0 129,629 - Atu0125 0.79 -0.4 129,718 - -1.4 129,741 + +1.2 129,753 + +0.5 129,963 + +0.9 129,976 - +0.4
Or see this region's nucleotide sequence