Experiment: AgroPhage_E10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0005 and Atu0006 are separated by 98 nucleotides Atu0006 and Atu0007 are separated by 103 nucleotides Atu0007 and Atu0008 are separated by 185 nucleotides Atu0008 and Atu0009 overlap by 4 nucleotides
Atu0005: Atu0005 - DNA polymerase III, epsilon chain, at 3,110 to 3,808
Atu0005
Atu0006: Atu0006 - protein-export protein SECB, at 3,907 to 4,389
Atu0006
Atu0007: Atu0007 - hypothetical protein, at 4,493 to 5,029
Atu0007
Atu0008: Atu0008 - hypothetical protein, at 5,215 to 5,868
Atu0008
Atu0009: Atu0009 - membrane-bound lytic murein transglycosylase, at 5,865 to 6,977
Atu0009
Position (kb) 4
5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 3.844 kb on + strand at 3.928 kb on - strand at 3.928 kb on - strand at 4.192 kb on + strand, within Atu0006 at 4.284 kb on - strand, within Atu0006 at 4.491 kb on + strand at 4.516 kb on + strand at 4.516 kb on + strand at 4.524 kb on - strand at 4.539 kb on - strand at 4.543 kb on + strand at 4.544 kb on + strand at 4.551 kb on - strand, within Atu0007 at 4.557 kb on + strand, within Atu0007 at 4.580 kb on + strand, within Atu0007 at 4.588 kb on + strand, within Atu0007 at 4.605 kb on + strand, within Atu0007 at 4.647 kb on + strand, within Atu0007 at 4.655 kb on - strand, within Atu0007 at 4.687 kb on + strand, within Atu0007 at 4.687 kb on + strand, within Atu0007 at 4.888 kb on + strand, within Atu0007 at 4.906 kb on - strand, within Atu0007 at 4.916 kb on - strand, within Atu0007 at 4.936 kb on + strand, within Atu0007 at 4.936 kb on + strand, within Atu0007 at 4.944 kb on - strand, within Atu0007 at 5.029 kb on + strand at 5.067 kb on + strand at 5.067 kb on + strand at 5.114 kb on + strand at 5.122 kb on - strand at 5.176 kb on - strand at 5.228 kb on - strand at 5.334 kb on + strand, within Atu0008 at 5.335 kb on + strand, within Atu0008 at 5.343 kb on - strand, within Atu0008 at 5.431 kb on - strand, within Atu0008 at 5.431 kb on - strand, within Atu0008 at 5.535 kb on + strand, within Atu0008 at 5.543 kb on - strand, within Atu0008 at 5.543 kb on - strand, within Atu0008 at 5.784 kb on + strand, within Atu0008 at 5.801 kb on - strand, within Atu0008 at 5.830 kb on - strand at 5.842 kb on - strand at 5.875 kb on - strand at 5.909 kb on + strand at 5.990 kb on - strand, within Atu0009 at 6.003 kb on + strand, within Atu0009
Per-strain Table
Position Strand Gene LocusTag Fraction AgroPhage_E10 remove 3,844 + +0.6 3,928 - +0.4 3,928 - +1.5 4,192 + Atu0006 0.59 +3.1 4,284 - Atu0006 0.78 +4.8 4,491 + -1.8 4,516 + +0.2 4,516 + -0.6 4,524 - -0.3 4,539 - -0.1 4,543 + +0.0 4,544 + -0.4 4,551 - Atu0007 0.11 +0.6 4,557 + Atu0007 0.12 +2.5 4,580 + Atu0007 0.16 -1.6 4,588 + Atu0007 0.18 -0.6 4,605 + Atu0007 0.21 -0.1 4,647 + Atu0007 0.29 -1.1 4,655 - Atu0007 0.30 -1.7 4,687 + Atu0007 0.36 -1.1 4,687 + Atu0007 0.36 -1.8 4,888 + Atu0007 0.74 -0.6 4,906 - Atu0007 0.77 -1.6 4,916 - Atu0007 0.79 +1.1 4,936 + Atu0007 0.82 +0.3 4,936 + Atu0007 0.82 -2.6 4,944 - Atu0007 0.84 -0.7 5,029 + +0.5 5,067 + +0.7 5,067 + -0.6 5,114 + +0.6 5,122 - -2.8 5,176 - -1.2 5,228 - -1.5 5,334 + Atu0008 0.18 -1.1 5,335 + Atu0008 0.18 -0.6 5,343 - Atu0008 0.20 +1.8 5,431 - Atu0008 0.33 +0.4 5,431 - Atu0008 0.33 -2.0 5,535 + Atu0008 0.49 -0.6 5,543 - Atu0008 0.50 -0.7 5,543 - Atu0008 0.50 +1.4 5,784 + Atu0008 0.87 -0.7 5,801 - Atu0008 0.90 -0.9 5,830 - +1.4 5,842 - +0.6 5,875 - +0.3 5,909 + -0.9 5,990 - Atu0009 0.11 -0.1 6,003 + Atu0009 0.12 +1.7
Or see this region's nucleotide sequence