Experiment: AgroPhage_D04
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5011 and Atu5013 are separated by 692 nucleotides Atu5013 and Atu5014 overlap by 4 nucleotides Atu5014 and Atu5015 are separated by 14 nucleotides
Atu5011: Atu5011 - polyketide biosynthesis dithiol-disulfide isomerase, at 13,082 to 13,723
Atu5011
Atu5013: Atu5013 - hypothetical protein, at 14,416 to 14,664
Atu5013
Atu5014: Atu5014 - plasmid stabilization protein, at 14,661 to 15,074
Atu5014
Atu5015: Atu5015 - phage-related integrase, at 15,089 to 16,129
Atu5015
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 13.859 kb on - strand at 13.865 kb on + strand at 13.870 kb on + strand at 13.870 kb on + strand at 13.883 kb on - strand at 13.971 kb on - strand at 13.976 kb on - strand at 14.631 kb on + strand, within Atu5013 at 14.809 kb on + strand, within Atu5014 at 14.844 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.901 kb on + strand, within Atu5014 at 14.909 kb on - strand, within Atu5014 at 14.958 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.990 kb on + strand, within Atu5014 at 14.998 kb on - strand, within Atu5014 at 14.998 kb on - strand, within Atu5014 at 15.167 kb on - strand at 15.261 kb on + strand, within Atu5015 at 15.269 kb on - strand, within Atu5015 at 15.308 kb on - strand, within Atu5015 at 15.502 kb on + strand, within Atu5015 at 15.502 kb on + strand, within Atu5015 at 15.559 kb on + strand, within Atu5015 at 15.672 kb on - strand, within Atu5015 at 15.752 kb on + strand, within Atu5015 at 15.760 kb on - strand, within Atu5015 at 15.760 kb on - strand, within Atu5015 at 15.760 kb on - strand, within Atu5015 at 15.770 kb on - strand, within Atu5015 at 15.802 kb on + strand, within Atu5015 at 15.993 kb on - strand, within Atu5015 at 15.995 kb on + strand, within Atu5015 at 15.998 kb on - strand, within Atu5015 at 15.998 kb on - strand, within Atu5015 at 16.035 kb on + strand at 16.035 kb on + strand at 16.045 kb on + strand at 16.066 kb on + strand at 16.066 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction AgroPhage_D04 remove 13,859 - +0.8 13,865 + -0.2 13,870 + -1.1 13,870 + -0.4 13,883 - -1.1 13,971 - +0.3 13,976 - +0.2 14,631 + Atu5013 0.86 +0.3 14,809 + Atu5014 0.36 -1.8 14,844 + Atu5014 0.44 -0.4 14,901 + Atu5014 0.58 +1.6 14,901 + Atu5014 0.58 -0.1 14,901 + Atu5014 0.58 +2.8 14,901 + Atu5014 0.58 -1.2 14,909 - Atu5014 0.60 -0.2 14,958 + Atu5014 0.72 -0.1 14,990 + Atu5014 0.79 +1.3 14,990 + Atu5014 0.79 +1.9 14,990 + Atu5014 0.79 -0.5 14,990 + Atu5014 0.79 -1.4 14,998 - Atu5014 0.81 +1.6 14,998 - Atu5014 0.81 +1.4 15,167 - +0.2 15,261 + Atu5015 0.17 -0.1 15,269 - Atu5015 0.17 -1.6 15,308 - Atu5015 0.21 -1.4 15,502 + Atu5015 0.40 -1.6 15,502 + Atu5015 0.40 +0.6 15,559 + Atu5015 0.45 -0.1 15,672 - Atu5015 0.56 -0.6 15,752 + Atu5015 0.64 -0.4 15,760 - Atu5015 0.64 -0.3 15,760 - Atu5015 0.64 -0.4 15,760 - Atu5015 0.64 -0.5 15,770 - Atu5015 0.65 -0.9 15,802 + Atu5015 0.68 -0.2 15,993 - Atu5015 0.87 -0.5 15,995 + Atu5015 0.87 -2.4 15,998 - Atu5015 0.87 -1.1 15,998 - Atu5015 0.87 -0.2 16,035 + -0.2 16,035 + -2.0 16,045 + -0.9 16,066 + +0.3 16,066 + -0.2
Or see this region's nucleotide sequence