Experiment: AgroPhage_D04
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu8175 and Atu2257 are separated by 1 nucleotides Atu2257 and Atu2258 are separated by 89 nucleotides Atu2258 and Atu2259 are separated by 9 nucleotides
Atu8175: Atu8175 - putative transmembrane protein, RNA-binding region RNP-1 (RNA recognition motif), at 2,226,253 to 2,229,063
Atu8175
Atu2257: Atu2257 - hypothetical protein, at 2,229,065 to 2,231,149
Atu2257
Atu2258: Atu2258 - acetyltransferase, at 2,231,239 to 2,231,652
Atu2258
Atu2259: Atu2259 - MFS permease, at 2,231,662 to 2,232,975
Atu2259
Position (kb)
2229
2230
2231
2232 Strain fitness (log2 ratio)
-1
0
1 at 2229.009 kb on + strand at 2229.017 kb on - strand at 2229.017 kb on - strand at 2229.017 kb on - strand at 2229.050 kb on + strand at 2229.068 kb on + strand at 2231.235 kb on - strand at 2231.407 kb on - strand, within Atu2258 at 2231.481 kb on + strand, within Atu2258 at 2231.589 kb on + strand, within Atu2258 at 2231.615 kb on - strand at 2231.668 kb on - strand at 2231.782 kb on - strand at 2231.900 kb on + strand, within Atu2259 at 2231.976 kb on + strand, within Atu2259
Per-strain Table
Position Strand Gene LocusTag Fraction AgroPhage_D04 remove 2,229,009 + -0.3 2,229,017 - +0.4 2,229,017 - +0.9 2,229,017 - +1.6 2,229,050 + -0.5 2,229,068 + -0.5 2,231,235 - +0.5 2,231,407 - Atu2258 0.41 -0.7 2,231,481 + Atu2258 0.58 -0.2 2,231,589 + Atu2258 0.85 -1.1 2,231,615 - -1.2 2,231,668 - -0.9 2,231,782 - -1.6 2,231,900 + Atu2259 0.18 -0.9 2,231,976 + Atu2259 0.24 +0.3
Or see this region's nucleotide sequence