Experiment: No phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0279 and Atu0280 are separated by 141 nucleotides Atu0280 and Atu0281 are separated by 74 nucleotides Atu0281 and Atu0282 are separated by 83 nucleotides Atu0282 and Atu0283 overlap by 4 nucleotides
Atu0279: Atu0279 - transcriptional regulator, TetR family, at 273,790 to 274,368
Atu0279
Atu0280: Atu0280 - hypothetical protein, at 274,510 to 274,998
Atu0280
Atu0281: Atu0281 - GMP synthase, at 275,073 to 276,650
Atu0281
Atu0282: Atu0282 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 276,734 to 277,372
Atu0282
Atu0283: Atu0283 - hypothetical protein, at 277,369 to 277,818
Atu0283
Position (kb) 275
276
277 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.145 kb on + strand, within Atu0279 at 274.208 kb on + strand, within Atu0279 at 274.223 kb on + strand, within Atu0279 at 274.223 kb on + strand, within Atu0279 at 274.248 kb on + strand, within Atu0279 at 274.248 kb on + strand, within Atu0279 at 274.248 kb on + strand, within Atu0279 at 274.278 kb on + strand, within Atu0279 at 274.283 kb on + strand, within Atu0279 at 274.353 kb on + strand at 274.361 kb on - strand at 274.376 kb on - strand at 274.433 kb on - strand at 274.446 kb on + strand at 274.544 kb on - strand at 274.663 kb on - strand, within Atu0280 at 274.791 kb on - strand, within Atu0280 at 274.861 kb on - strand, within Atu0280 at 274.882 kb on + strand, within Atu0280 at 275.019 kb on - strand at 275.019 kb on - strand at 275.023 kb on + strand at 275.023 kb on + strand at 275.035 kb on + strand at 276.752 kb on + strand at 276.775 kb on + strand at 276.896 kb on + strand, within Atu0282 at 276.906 kb on + strand, within Atu0282 at 276.957 kb on + strand, within Atu0282 at 276.963 kb on - strand, within Atu0282 at 276.965 kb on - strand, within Atu0282 at 277.130 kb on + strand, within Atu0282 at 277.273 kb on - strand, within Atu0282 at 277.273 kb on - strand, within Atu0282 at 277.409 kb on - strand at 277.452 kb on + strand, within Atu0283 at 277.452 kb on + strand, within Atu0283 at 277.452 kb on + strand, within Atu0283 at 277.460 kb on - strand, within Atu0283 at 277.460 kb on - strand, within Atu0283 at 277.460 kb on - strand, within Atu0283 at 277.460 kb on - strand, within Atu0283 at 277.548 kb on + strand, within Atu0283 at 277.567 kb on + strand, within Atu0283 at 277.579 kb on + strand, within Atu0283
Per-strain Table
Position Strand Gene LocusTag Fraction No phage control remove 274,145 + Atu0279 0.61 +0.3 274,208 + Atu0279 0.72 -0.5 274,223 + Atu0279 0.75 +0.2 274,223 + Atu0279 0.75 -0.3 274,248 + Atu0279 0.79 -0.3 274,248 + Atu0279 0.79 -0.3 274,248 + Atu0279 0.79 +0.2 274,278 + Atu0279 0.84 +2.0 274,283 + Atu0279 0.85 +0.2 274,353 + +1.0 274,361 - -2.1 274,376 - -0.8 274,433 - -1.7 274,446 + -0.4 274,544 - -1.0 274,663 - Atu0280 0.31 -0.5 274,791 - Atu0280 0.57 -0.0 274,861 - Atu0280 0.72 +2.2 274,882 + Atu0280 0.76 -1.8 275,019 - +0.7 275,019 - +0.5 275,023 + +0.6 275,023 + -0.1 275,035 + -0.5 276,752 + +0.4 276,775 + -0.4 276,896 + Atu0282 0.25 -0.5 276,906 + Atu0282 0.27 +0.8 276,957 + Atu0282 0.35 -0.5 276,963 - Atu0282 0.36 +0.2 276,965 - Atu0282 0.36 +0.2 277,130 + Atu0282 0.62 -0.6 277,273 - Atu0282 0.84 -0.2 277,273 - Atu0282 0.84 +0.7 277,409 - -0.3 277,452 + Atu0283 0.18 -0.4 277,452 + Atu0283 0.18 -0.8 277,452 + Atu0283 0.18 -2.0 277,460 - Atu0283 0.20 +0.8 277,460 - Atu0283 0.20 +1.6 277,460 - Atu0283 0.20 -2.8 277,460 - Atu0283 0.20 -0.6 277,548 + Atu0283 0.40 -0.3 277,567 + Atu0283 0.44 -0.3 277,579 + Atu0283 0.47 +0.8
Or see this region's nucleotide sequence