Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfH and b1591 are separated by 42 nucleotides b1591 and b1592 are separated by 194 nucleotides b1592 and b1593 overlap by 48 nucleotides b1593 and dgsA are separated by 124 nucleotides
b1590: ynfH - oxidoreductase, membrane subunit (NCBI), at 1,661,633 to 1,662,487
ynfH
b1591: b1591 - putative oxidoreductase component (VIMSS), at 1,662,530 to 1,663,144
b1591
b1592: b1592 - putative chloride channel (VIMSS), at 1,663,339 to 1,664,595
b1592
b1593: b1593 - orf, hypothetical protein (VIMSS), at 1,664,548 to 1,665,243
b1593
b1594: dgsA - DNA-binding transcriptional repressor (NCBI), at 1,665,368 to 1,666,588
dgsA
Position (kb)
1663
1664
1665 Strain fitness (log2 ratio)
-1
0
1
2 at 1662.405 kb on + strand at 1662.482 kb on + strand at 1662.522 kb on + strand at 1662.589 kb on + strand at 1662.796 kb on + strand, within b1591 at 1662.877 kb on + strand, within b1591 at 1663.574 kb on + strand, within b1592 at 1663.574 kb on + strand, within b1592 at 1663.876 kb on - strand, within b1592 at 1663.921 kb on - strand, within b1592 at 1663.990 kb on - strand, within b1592 at 1663.990 kb on - strand, within b1592 at 1664.007 kb on + strand, within b1592 at 1664.007 kb on + strand, within b1592 at 1664.013 kb on + strand, within b1592 at 1664.279 kb on + strand, within b1592 at 1664.301 kb on + strand, within b1592 at 1664.341 kb on + strand, within b1592 at 1664.343 kb on - strand, within b1592 at 1664.359 kb on + strand, within b1592 at 1664.448 kb on + strand, within b1592 at 1664.448 kb on + strand, within b1592 at 1664.579 kb on - strand at 1664.586 kb on + strand at 1664.586 kb on + strand at 1664.976 kb on + strand, within b1593 at 1664.983 kb on + strand, within b1593 at 1664.983 kb on + strand, within b1593 at 1664.991 kb on - strand, within b1593 at 1664.991 kb on - strand, within b1593 at 1664.991 kb on - strand, within b1593 at 1665.407 kb on - strand at 1665.479 kb on - strand at 1665.479 kb on - strand at 1665.479 kb on - strand at 1665.479 kb on - strand at 1665.493 kb on - strand, within dgsA
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 1,662,405 + +0.4 1,662,482 + +0.8 1,662,522 + -0.0 1,662,589 + +0.2 1,662,796 + b1591 b1591 0.43 +0.8 1,662,877 + b1591 b1591 0.56 +0.3 1,663,574 + b1592 b1592 0.19 +0.5 1,663,574 + b1592 b1592 0.19 +1.1 1,663,876 - b1592 b1592 0.43 -0.1 1,663,921 - b1592 b1592 0.46 +0.8 1,663,990 - b1592 b1592 0.52 +0.3 1,663,990 - b1592 b1592 0.52 +0.4 1,664,007 + b1592 b1592 0.53 +0.2 1,664,007 + b1592 b1592 0.53 +0.8 1,664,013 + b1592 b1592 0.54 +1.3 1,664,279 + b1592 b1592 0.75 +0.6 1,664,301 + b1592 b1592 0.77 +1.0 1,664,341 + b1592 b1592 0.80 +0.3 1,664,343 - b1592 b1592 0.80 +0.2 1,664,359 + b1592 b1592 0.81 -0.1 1,664,448 + b1592 b1592 0.88 +0.9 1,664,448 + b1592 b1592 0.88 -0.8 1,664,579 - +0.3 1,664,586 + +0.7 1,664,586 + +0.7 1,664,976 + b1593 b1593 0.61 +0.9 1,664,983 + b1593 b1593 0.62 -0.5 1,664,983 + b1593 b1593 0.62 +2.7 1,664,991 - b1593 b1593 0.64 +0.7 1,664,991 - b1593 b1593 0.64 +0.0 1,664,991 - b1593 b1593 0.64 +0.3 1,665,407 - -1.2 1,665,479 - +0.1 1,665,479 - -0.2 1,665,479 - -1.2 1,665,479 - -0.1 1,665,493 - dgsA b1594 0.10 -0.3
Or see this region's nucleotide sequence