Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1470 and PP_1471 are separated by 59 nucleotides PP_1471 and PP_5471 are separated by 130 nucleotides PP_5471 and PP_1473 are separated by 79 nucleotides
PP_1470: PP_1470 - Homoserine dehydrogenase, at 1,674,834 to 1,676,138
_1470
PP_1471: PP_1471 - Threonine synthase, at 1,676,198 to 1,677,607
_1471
PP_5471: PP_5471 - putative response regulator receiver protein, at 1,677,738 to 1,678,157
_5471
PP_1473: PP_1473 - conserved protein of unknown function, at 1,678,237 to 1,678,518
_1473
Position (kb)
1676
1677
1678 Strain fitness (log2 ratio)
-1
0
1
2 at 1675.716 kb on + strand at 1675.716 kb on + strand, within PP_1470 at 1675.733 kb on + strand at 1675.742 kb on + strand at 1675.887 kb on + strand, within PP_1470 at 1675.890 kb on + strand at 1676.143 kb on + strand at 1677.606 kb on - strand at 1677.666 kb on + strand at 1677.666 kb on + strand at 1677.681 kb on - strand at 1677.681 kb on - strand at 1677.681 kb on - strand at 1677.706 kb on - strand at 1677.706 kb on - strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.750 kb on + strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.751 kb on - strand at 1677.788 kb on - strand, within PP_5471 at 1677.788 kb on - strand, within PP_5471 at 1678.204 kb on - strand at 1678.208 kb on + strand at 1678.208 kb on + strand at 1678.208 kb on + strand at 1678.209 kb on - strand at 1678.209 kb on - strand at 1678.209 kb on - strand at 1678.315 kb on - strand, within PP_1473 at 1678.315 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.351 kb on - strand, within PP_1473 at 1678.353 kb on + strand, within PP_1473 at 1678.443 kb on - strand, within PP_1473 at 1678.537 kb on + strand at 1678.537 kb on + strand at 1678.537 kb on + strand at 1678.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,675,716 + +2.2 1,675,716 + PP_1470 0.68 +0.8 1,675,733 + +0.8 1,675,742 + +0.2 1,675,887 + PP_1470 0.81 +0.2 1,675,890 + +1.2 1,676,143 + +0.6 1,677,606 - +0.0 1,677,666 + +0.1 1,677,666 + -0.2 1,677,681 - +0.1 1,677,681 - -0.0 1,677,681 - +0.1 1,677,706 - +0.3 1,677,706 - -1.1 1,677,750 + +0.7 1,677,750 + -0.9 1,677,750 + -0.2 1,677,750 + -0.1 1,677,751 - +0.7 1,677,751 - -0.7 1,677,751 - -0.0 1,677,751 - +0.6 1,677,751 - -1.0 1,677,788 - PP_5471 0.12 +0.1 1,677,788 - PP_5471 0.12 +0.3 1,678,204 - -0.4 1,678,208 + +0.3 1,678,208 + +0.1 1,678,208 + -1.2 1,678,209 - -0.1 1,678,209 - -0.2 1,678,209 - -0.1 1,678,315 - PP_1473 0.28 +0.6 1,678,315 - PP_1473 0.28 +0.4 1,678,351 - PP_1473 0.40 -0.5 1,678,351 - PP_1473 0.40 -0.1 1,678,351 - PP_1473 0.40 -1.6 1,678,353 + PP_1473 0.41 +0.1 1,678,443 - PP_1473 0.73 -0.3 1,678,537 + -0.2 1,678,537 + +0.3 1,678,537 + +0.9 1,678,538 - -0.8
Or see this region's nucleotide sequence