Strain Fitness in Pseudomonas putida KT2440 around PP_2465

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2463 and PP_5525 are separated by 162 nucleotidesPP_5525 and PP_2465 are separated by 338 nucleotidesPP_2465 and PP_2466 are separated by 17 nucleotidesPP_2466 and PP_2467 are separated by 45 nucleotidesPP_2467 and PP_2468 are separated by 27 nucleotides PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787 _2463 PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255 _5525 PP_2465: PP_2465 - Threonine--tRNA ligase, at 2,809,594 to 2,811,516 _2465 PP_2466: PP_2466 - Translation initiation factor IF-3, at 2,811,534 to 2,812,067 _2466 PP_2467: PP_2467 - 50S ribosomal subunit protein L35, at 2,812,113 to 2,812,322 _2467 PP_2468: PP_2468 - 50S ribosomal protein L20, at 2,812,350 to 2,812,706 _2468 Position (kb) 2809 2810 2811 2812Strain fitness (log2 ratio) -1 0 1 2at 2808.693 kb on - strand, within PP_2463at 2808.808 kb on + strandat 2808.809 kb on - strandat 2808.866 kb on + strandat 2808.880 kb on + strandat 2808.880 kb on + strandat 2808.890 kb on + strandat 2808.891 kb on - strandat 2808.944 kb on - strandat 2809.155 kb on + strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.156 kb on - strand, within PP_5525at 2809.263 kb on + strandat 2809.264 kb on - strandat 2809.292 kb on + strandat 2809.292 kb on + strandat 2809.293 kb on - strandat 2809.332 kb on - strandat 2809.332 kb on - strandat 2809.389 kb on + strandat 2809.389 kb on + strandat 2809.430 kb on + strandat 2809.533 kb on + strandat 2812.095 kb on + strandat 2812.095 kb on + strandat 2812.095 kb on + strandat 2812.095 kb on + strandat 2812.095 kb on + strandat 2812.095 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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2,808,693 - PP_2463 0.55 +1.3
2,808,808 + -0.4
2,808,809 - -1.1
2,808,866 + +0.6
2,808,880 + +1.0
2,808,880 + +1.2
2,808,890 + -0.5
2,808,891 - -0.0
2,808,944 - -0.8
2,809,155 + PP_5525 0.67 -0.9
2,809,156 - PP_5525 0.67 -1.4
2,809,156 - PP_5525 0.67 -0.8
2,809,156 - PP_5525 0.67 +0.5
2,809,156 - PP_5525 0.67 -0.6
2,809,156 - PP_5525 0.67 +0.6
2,809,263 + -0.1
2,809,264 - +1.6
2,809,292 + +0.2
2,809,292 + -0.0
2,809,293 - -0.0
2,809,332 - -0.9
2,809,332 - -0.0
2,809,389 + +2.2
2,809,389 + +0.1
2,809,430 + +1.0
2,809,533 + +0.5
2,812,095 + -0.4
2,812,095 + -1.2
2,812,095 + +0.5
2,812,095 + +0.3
2,812,095 + +0.2
2,812,095 + +0.6

Or see this region's nucleotide sequence