Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2463 and PP_5525 are separated by 162 nucleotides PP_5525 and PP_2465 are separated by 338 nucleotides PP_2465 and PP_2466 are separated by 17 nucleotides PP_2466 and PP_2467 are separated by 45 nucleotides PP_2467 and PP_2468 are separated by 27 nucleotides
PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787
_2463
PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255
_5525
PP_2465: PP_2465 - Threonine--tRNA ligase, at 2,809,594 to 2,811,516
_2465
PP_2466: PP_2466 - Translation initiation factor IF-3, at 2,811,534 to 2,812,067
_2466
PP_2467: PP_2467 - 50S ribosomal subunit protein L35, at 2,812,113 to 2,812,322
_2467
PP_2468: PP_2468 - 50S ribosomal protein L20, at 2,812,350 to 2,812,706
_2468
Position (kb)
2809
2810
2811
2812 Strain fitness (log2 ratio)
-1
0
1
2 at 2808.693 kb on - strand, within PP_2463 at 2808.808 kb on + strand at 2808.809 kb on - strand at 2808.866 kb on + strand at 2808.880 kb on + strand at 2808.880 kb on + strand at 2808.890 kb on + strand at 2808.891 kb on - strand at 2808.944 kb on - strand at 2809.155 kb on + strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.156 kb on - strand, within PP_5525 at 2809.263 kb on + strand at 2809.264 kb on - strand at 2809.292 kb on + strand at 2809.292 kb on + strand at 2809.293 kb on - strand at 2809.332 kb on - strand at 2809.332 kb on - strand at 2809.389 kb on + strand at 2809.389 kb on + strand at 2809.430 kb on + strand at 2809.533 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand at 2812.095 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,808,693 - PP_2463 0.55 +1.3 2,808,808 + -0.4 2,808,809 - -1.1 2,808,866 + +0.6 2,808,880 + +1.0 2,808,880 + +1.2 2,808,890 + -0.5 2,808,891 - -0.0 2,808,944 - -0.8 2,809,155 + PP_5525 0.67 -0.9 2,809,156 - PP_5525 0.67 -1.4 2,809,156 - PP_5525 0.67 -0.8 2,809,156 - PP_5525 0.67 +0.5 2,809,156 - PP_5525 0.67 -0.6 2,809,156 - PP_5525 0.67 +0.6 2,809,263 + -0.1 2,809,264 - +1.6 2,809,292 + +0.2 2,809,292 + -0.0 2,809,293 - -0.0 2,809,332 - -0.9 2,809,332 - -0.0 2,809,389 + +2.2 2,809,389 + +0.1 2,809,430 + +1.0 2,809,533 + +0.5 2,812,095 + -0.4 2,812,095 + -1.2 2,812,095 + +0.5 2,812,095 + +0.3 2,812,095 + +0.2 2,812,095 + +0.6
Or see this region's nucleotide sequence