Strain Fitness in Pseudomonas putida KT2440 around PP_1997

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1996 and PP_1997 overlap by 4 nucleotidesPP_1997 and PP_1998 overlap by 17 nucleotidesPP_1998 and PP_1999 are separated by 196 nucleotides PP_1996: PP_1996 - acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, at 2,264,051 to 2,264,944 _1996 PP_1997: PP_1997 - folypolyglutamate synthase/dihydrofolate synthase, at 2,264,941 to 2,266,251 _1997 PP_1998: PP_1998 - putative cell division protein, at 2,266,235 to 2,266,879 _1998 PP_1999: PP_1999 - membrane protein required for colicin V production, at 2,267,076 to 2,267,633 _1999 Position (kb) 2264 2265 2266 2267Strain fitness (log2 ratio) -2 -1 0 1at 2266.232 kb on + strandat 2266.232 kb on + strandat 2266.232 kb on + strandat 2266.233 kb on - strandat 2266.303 kb on + strand, within PP_1998at 2266.435 kb on + strand, within PP_1998at 2266.436 kb on - strand, within PP_1998at 2266.495 kb on + strand, within PP_1998at 2266.495 kb on + strand, within PP_1998at 2266.717 kb on + strand, within PP_1998at 2266.727 kb on + strand, within PP_1998at 2266.727 kb on + strand, within PP_1998at 2266.727 kb on + strand, within PP_1998at 2266.728 kb on - strand, within PP_1998at 2266.728 kb on - strand, within PP_1998at 2266.728 kb on - strandat 2266.736 kb on + strand, within PP_1998at 2266.736 kb on + strand, within PP_1998at 2266.736 kb on + strand, within PP_1998at 2266.736 kb on + strand, within PP_1998at 2266.737 kb on - strand, within PP_1998at 2266.737 kb on - strand, within PP_1998at 2266.737 kb on - strand, within PP_1998at 2266.738 kb on + strand, within PP_1998at 2266.769 kb on + strand, within PP_1998at 2266.770 kb on - strand, within PP_1998at 2266.931 kb on + strandat 2267.084 kb on + strandat 2267.084 kb on + strandat 2267.232 kb on + strand, within PP_1999at 2267.244 kb on + strand, within PP_1999at 2267.244 kb on + strand, within PP_1999

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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2,266,232 + +0.7
2,266,232 + +0.8
2,266,232 + +0.7
2,266,233 - -0.9
2,266,303 + PP_1998 0.11 -0.1
2,266,435 + PP_1998 0.31 -1.9
2,266,436 - PP_1998 0.31 -1.3
2,266,495 + PP_1998 0.40 -1.1
2,266,495 + PP_1998 0.40 +1.4
2,266,717 + PP_1998 0.75 -0.4
2,266,727 + PP_1998 0.76 -0.3
2,266,727 + PP_1998 0.76 -0.7
2,266,727 + PP_1998 0.76 -0.2
2,266,728 - PP_1998 0.76 -0.3
2,266,728 - PP_1998 0.76 +0.4
2,266,728 - +1.1
2,266,736 + PP_1998 0.78 +0.7
2,266,736 + PP_1998 0.78 -1.5
2,266,736 + PP_1998 0.78 -0.1
2,266,736 + PP_1998 0.78 -0.2
2,266,737 - PP_1998 0.78 +1.0
2,266,737 - PP_1998 0.78 -0.9
2,266,737 - PP_1998 0.78 -0.2
2,266,738 + PP_1998 0.78 -0.5
2,266,769 + PP_1998 0.83 -0.8
2,266,770 - PP_1998 0.83 -0.7
2,266,931 + +0.7
2,267,084 + +1.0
2,267,084 + -0.3
2,267,232 + PP_1999 0.28 +0.8
2,267,244 + PP_1999 0.30 -2.4
2,267,244 + PP_1999 0.30 +0.9

Or see this region's nucleotide sequence