Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzyE and rffM are separated by 2 nucleotides rffM and yifK are separated by 190 nucleotides
b3793: wzyE - putative enterobacterial common antigen polymerase (NCBI), at 3,976,624 to 3,977,976
wzyE
b3794: rffM - putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI), at 3,977,979 to 3,978,719
rffM
b3795: yifK - predicted transporter (NCBI), at 3,978,910 to 3,980,295
yifK
Position (kb)
3977
3978
3979 Strain fitness (log2 ratio)
-2
-1
0
1 at 3978.002 kb on + strand at 3978.004 kb on + strand at 3978.004 kb on + strand at 3978.004 kb on + strand at 3978.012 kb on - strand at 3978.017 kb on - strand at 3978.017 kb on - strand at 3978.024 kb on + strand at 3978.056 kb on - strand, within rffM at 3978.066 kb on - strand, within rffM at 3978.066 kb on - strand, within rffM at 3978.083 kb on + strand, within rffM at 3978.090 kb on - strand, within rffM at 3978.138 kb on - strand, within rffM at 3978.138 kb on - strand, within rffM at 3978.140 kb on - strand, within rffM at 3978.167 kb on - strand, within rffM at 3978.199 kb on + strand, within rffM at 3978.199 kb on + strand, within rffM at 3978.202 kb on - strand, within rffM at 3978.249 kb on - strand, within rffM at 3978.274 kb on + strand, within rffM at 3978.274 kb on + strand, within rffM at 3978.291 kb on + strand, within rffM at 3978.314 kb on - strand, within rffM at 3978.314 kb on - strand, within rffM at 3978.323 kb on + strand, within rffM at 3978.323 kb on + strand, within rffM at 3978.472 kb on + strand, within rffM at 3978.472 kb on + strand, within rffM at 3978.540 kb on + strand, within rffM at 3978.540 kb on + strand, within rffM at 3978.542 kb on + strand, within rffM at 3978.542 kb on + strand, within rffM at 3978.542 kb on + strand, within rffM at 3978.550 kb on - strand, within rffM at 3978.570 kb on + strand, within rffM at 3978.618 kb on + strand, within rffM at 3978.618 kb on + strand, within rffM at 3978.693 kb on - strand at 3978.693 kb on - strand at 3978.698 kb on + strand at 3978.709 kb on - strand at 3978.753 kb on - strand at 3978.858 kb on - strand at 3978.858 kb on - strand at 3978.863 kb on + strand at 3978.886 kb on + strand at 3978.886 kb on + strand at 3978.896 kb on - strand at 3978.928 kb on + strand at 3978.944 kb on - strand at 3978.956 kb on + strand at 3978.956 kb on + strand at 3978.970 kb on + strand at 3978.970 kb on + strand at 3978.978 kb on - strand at 3978.978 kb on - strand at 3978.978 kb on - strand at 3978.979 kb on - strand at 3978.999 kb on + strand at 3979.011 kb on + strand at 3979.024 kb on - strand at 3979.028 kb on - strand at 3979.045 kb on - strand at 3979.045 kb on - strand at 3979.047 kb on - strand at 3979.047 kb on - strand at 3979.058 kb on - strand, within yifK at 3979.063 kb on - strand, within yifK at 3979.153 kb on + strand, within yifK at 3979.176 kb on - strand, within yifK at 3979.185 kb on + strand, within yifK at 3979.186 kb on - strand, within yifK at 3979.186 kb on - strand, within yifK at 3979.199 kb on + strand, within yifK at 3979.212 kb on - strand, within yifK at 3979.281 kb on + strand, within yifK at 3979.281 kb on + strand, within yifK at 3979.309 kb on - strand, within yifK at 3979.344 kb on - strand, within yifK at 3979.344 kb on - strand, within yifK at 3979.344 kb on - strand, within yifK at 3979.344 kb on - strand, within yifK at 3979.472 kb on + strand, within yifK at 3979.479 kb on - strand, within yifK at 3979.527 kb on + strand, within yifK at 3979.527 kb on + strand, within yifK at 3979.553 kb on - strand, within yifK at 3979.599 kb on + strand, within yifK at 3979.696 kb on + strand, within yifK at 3979.696 kb on + strand, within yifK at 3979.717 kb on - strand, within yifK
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 3,978,002 + +0.1 3,978,004 + -2.0 3,978,004 + -0.3 3,978,004 + -0.2 3,978,012 - +0.5 3,978,017 - -0.6 3,978,017 - -0.3 3,978,024 + -0.7 3,978,056 - rffM b3794 0.10 +0.2 3,978,066 - rffM b3794 0.12 +0.1 3,978,066 - rffM b3794 0.12 -0.7 3,978,083 + rffM b3794 0.14 -0.6 3,978,090 - rffM b3794 0.15 -0.3 3,978,138 - rffM b3794 0.21 -0.5 3,978,138 - rffM b3794 0.21 -1.2 3,978,140 - rffM b3794 0.22 -0.7 3,978,167 - rffM b3794 0.25 +0.3 3,978,199 + rffM b3794 0.30 -0.4 3,978,199 + rffM b3794 0.30 -0.8 3,978,202 - rffM b3794 0.30 -0.4 3,978,249 - rffM b3794 0.36 -1.7 3,978,274 + rffM b3794 0.40 +0.9 3,978,274 + rffM b3794 0.40 -0.6 3,978,291 + rffM b3794 0.42 -0.3 3,978,314 - rffM b3794 0.45 -0.0 3,978,314 - rffM b3794 0.45 -0.5 3,978,323 + rffM b3794 0.46 -0.4 3,978,323 + rffM b3794 0.46 -1.6 3,978,472 + rffM b3794 0.67 -1.0 3,978,472 + rffM b3794 0.67 -0.2 3,978,540 + rffM b3794 0.76 -1.0 3,978,540 + rffM b3794 0.76 +0.0 3,978,542 + rffM b3794 0.76 -1.6 3,978,542 + rffM b3794 0.76 -0.6 3,978,542 + rffM b3794 0.76 -0.9 3,978,550 - rffM b3794 0.77 -0.5 3,978,570 + rffM b3794 0.80 +0.5 3,978,618 + rffM b3794 0.86 -0.1 3,978,618 + rffM b3794 0.86 -0.6 3,978,693 - -0.5 3,978,693 - +1.1 3,978,698 + +0.3 3,978,709 - -0.1 3,978,753 - -0.0 3,978,858 - +0.6 3,978,858 - +0.8 3,978,863 + +0.4 3,978,886 + -0.5 3,978,886 + +0.2 3,978,896 - -0.9 3,978,928 + -0.4 3,978,944 - +0.9 3,978,956 + +0.4 3,978,956 + -0.3 3,978,970 + -0.3 3,978,970 + +0.5 3,978,978 - -0.1 3,978,978 - +0.4 3,978,978 - +0.0 3,978,979 - +0.6 3,978,999 + +0.0 3,979,011 + +0.0 3,979,024 - -0.6 3,979,028 - +0.4 3,979,045 - +0.6 3,979,045 - -0.4 3,979,047 - +0.5 3,979,047 - +0.3 3,979,058 - yifK b3795 0.11 -0.6 3,979,063 - yifK b3795 0.11 -0.2 3,979,153 + yifK b3795 0.18 +0.3 3,979,176 - yifK b3795 0.19 -0.0 3,979,185 + yifK b3795 0.20 +0.3 3,979,186 - yifK b3795 0.20 -0.3 3,979,186 - yifK b3795 0.20 +0.8 3,979,199 + yifK b3795 0.21 +0.3 3,979,212 - yifK b3795 0.22 -0.4 3,979,281 + yifK b3795 0.27 +0.5 3,979,281 + yifK b3795 0.27 +0.4 3,979,309 - yifK b3795 0.29 -1.1 3,979,344 - yifK b3795 0.31 -0.3 3,979,344 - yifK b3795 0.31 +0.4 3,979,344 - yifK b3795 0.31 -1.9 3,979,344 - yifK b3795 0.31 +0.2 3,979,472 + yifK b3795 0.41 -0.0 3,979,479 - yifK b3795 0.41 -0.3 3,979,527 + yifK b3795 0.45 -0.4 3,979,527 + yifK b3795 0.45 +0.3 3,979,553 - yifK b3795 0.46 +0.1 3,979,599 + yifK b3795 0.50 -0.3 3,979,696 + yifK b3795 0.57 +0.3 3,979,696 + yifK b3795 0.57 -0.3 3,979,717 - yifK b3795 0.58 +0.7
Or see this region's nucleotide sequence