Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recD and recB overlap by 1 nucleotides recB and ptr overlap by 8 nucleotides
b2819: recD - exonuclease V (RecBCD complex), alpha chain (NCBI), at 2,948,657 to 2,950,483
recD
b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025
recB
b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906
ptr
Position (kb)
2950
2951
2952
2953
2954
2955 Strain fitness (log2 ratio)
-1
0
1 at 2949.559 kb on - strand, within recD at 2949.564 kb on - strand, within recD at 2949.648 kb on + strand, within recD at 2949.648 kb on - strand, within recD at 2949.648 kb on - strand, within recD at 2949.658 kb on + strand, within recD at 2949.691 kb on - strand, within recD at 2949.691 kb on - strand, within recD at 2949.911 kb on - strand, within recD at 2950.492 kb on + strand at 2950.492 kb on + strand at 2950.500 kb on - strand at 2951.352 kb on - strand, within recB at 2951.941 kb on + strand, within recB at 2952.471 kb on - strand, within recB at 2952.820 kb on + strand, within recB at 2953.040 kb on - strand, within recB at 2953.978 kb on + strand at 2953.978 kb on + strand at 2954.006 kb on - strand at 2954.072 kb on + strand at 2954.072 kb on + strand at 2954.201 kb on - strand at 2954.359 kb on + strand, within ptr at 2954.508 kb on + strand, within ptr at 2954.516 kb on + strand, within ptr at 2954.587 kb on - strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.713 kb on - strand, within ptr at 2954.724 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.777 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 2,949,559 - recD b2819 0.49 -0.3 2,949,564 - recD b2819 0.50 +0.4 2,949,648 + recD b2819 0.54 -0.9 2,949,648 - recD b2819 0.54 -0.1 2,949,648 - recD b2819 0.54 -0.4 2,949,658 + recD b2819 0.55 -0.2 2,949,691 - recD b2819 0.57 +0.2 2,949,691 - recD b2819 0.57 -1.1 2,949,911 - recD b2819 0.69 -0.1 2,950,492 + +0.2 2,950,492 + +1.0 2,950,500 - -0.7 2,951,352 - recB b2820 0.25 -1.3 2,951,941 + recB b2820 0.41 +0.3 2,952,471 - recB b2820 0.56 +0.3 2,952,820 + recB b2820 0.66 -1.0 2,953,040 - recB b2820 0.72 -1.7 2,953,978 + -0.2 2,953,978 + +0.3 2,954,006 - -0.7 2,954,072 + -0.3 2,954,072 + -0.4 2,954,201 - +0.2 2,954,359 + ptr b2821 0.12 -0.2 2,954,508 + ptr b2821 0.17 +0.8 2,954,516 + ptr b2821 0.17 +0.4 2,954,587 - ptr b2821 0.20 +0.6 2,954,617 + ptr b2821 0.21 -0.6 2,954,617 + ptr b2821 0.21 +0.4 2,954,626 - ptr b2821 0.21 +0.2 2,954,626 - ptr b2821 0.21 -1.2 2,954,713 - ptr b2821 0.24 -0.7 2,954,724 - ptr b2821 0.24 -0.1 2,954,759 - ptr b2821 0.26 +0.2 2,954,759 - ptr b2821 0.26 +0.0 2,954,771 - ptr b2821 0.26 +0.5 2,954,771 - ptr b2821 0.26 +0.3 2,954,777 - ptr b2821 0.26 +0.4 2,954,782 - ptr b2821 0.26 +0.6 2,954,782 - ptr b2821 0.26 -0.5 2,954,857 + ptr b2821 0.29 -0.3 2,954,857 + ptr b2821 0.29 -1.3 2,954,905 + ptr b2821 0.31 +0.1 2,954,905 + ptr b2821 0.31 +0.3 2,954,985 + ptr b2821 0.33 +0.2 2,954,985 + ptr b2821 0.33 +0.0
Or see this region's nucleotide sequence