Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2431 and yfeY are separated by 95 nucleotides yfeY and b2433 are separated by 60 nucleotides b2433 and b2434 overlap by 14 nucleotides b2434 and amiA are separated by 213 nucleotides
b2431: b2431 - orf, hypothetical protein (VIMSS), at 2,547,668 to 2,548,567
b2431
b2432: yfeY - hypothetical protein (NCBI), at 2,548,663 to 2,549,238
yfeY
b2433: b2433 - orf, hypothetical protein (VIMSS), at 2,549,299 to 2,549,748
b2433
b2434: b2434 - orf, hypothetical protein (VIMSS), at 2,549,735 to 2,550,160
b2434
b2435: amiA - N-acetylmuramoyl-l-alanine amidase I (NCBI), at 2,550,374 to 2,551,243
amiA
Position (kb)
2549
2550 Strain fitness (log2 ratio)
-2
-1
0
1 at 2548.322 kb on - strand, within b2431 at 2548.537 kb on - strand at 2548.537 kb on - strand at 2548.895 kb on + strand, within yfeY at 2549.078 kb on + strand, within yfeY at 2549.078 kb on + strand, within yfeY at 2549.210 kb on + strand at 2549.253 kb on - strand at 2549.266 kb on - strand at 2549.348 kb on + strand, within b2433 at 2549.388 kb on + strand, within b2433 at 2549.645 kb on + strand, within b2433 at 2549.680 kb on + strand, within b2433 at 2549.680 kb on + strand, within b2433 at 2549.746 kb on - strand at 2549.795 kb on + strand, within b2434 at 2549.833 kb on - strand, within b2434 at 2549.864 kb on + strand, within b2434 at 2550.001 kb on + strand, within b2434 at 2550.037 kb on + strand, within b2434 at 2550.357 kb on + strand at 2550.357 kb on + strand at 2550.381 kb on + strand at 2550.597 kb on - strand, within amiA at 2550.631 kb on - strand, within amiA at 2550.747 kb on + strand, within amiA
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 2,548,322 - b2431 b2431 0.73 +0.0 2,548,537 - +0.6 2,548,537 - -0.5 2,548,895 + yfeY b2432 0.40 -0.5 2,549,078 + yfeY b2432 0.72 -1.2 2,549,078 + yfeY b2432 0.72 +0.1 2,549,210 + -0.9 2,549,253 - -0.2 2,549,266 - +0.4 2,549,348 + b2433 b2433 0.11 -0.7 2,549,388 + b2433 b2433 0.20 -0.1 2,549,645 + b2433 b2433 0.77 -0.1 2,549,680 + b2433 b2433 0.85 -2.2 2,549,680 + b2433 b2433 0.85 -0.5 2,549,746 - +0.2 2,549,795 + b2434 b2434 0.14 -0.2 2,549,833 - b2434 b2434 0.23 -0.1 2,549,864 + b2434 b2434 0.30 +0.3 2,550,001 + b2434 b2434 0.62 -0.1 2,550,037 + b2434 b2434 0.71 -0.0 2,550,357 + +1.3 2,550,357 + +0.1 2,550,381 + +1.1 2,550,597 - amiA b2435 0.26 +0.1 2,550,631 - amiA b2435 0.30 -0.6 2,550,747 + amiA b2435 0.43 -0.1
Or see this region's nucleotide sequence