Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and b1762 are separated by 116 nucleotides b1762 and topB are separated by 127 nucleotides
b1761: gdhA - glutamate dehydrogenase (NCBI), at 1,840,395 to 1,841,738
gdhA
b1762: b1762 - orf, hypothetical protein (VIMSS), at 1,841,855 to 1,842,895
b1762
b1763: topB - DNA topoisomerase III (NCBI), at 1,843,023 to 1,844,984
topB
Position (kb)
1841
1842
1843 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1840.876 kb on - strand, within gdhA at 1840.886 kb on + strand, within gdhA at 1840.886 kb on + strand, within gdhA at 1840.971 kb on + strand, within gdhA at 1840.971 kb on + strand, within gdhA at 1841.012 kb on - strand, within gdhA at 1841.111 kb on + strand, within gdhA at 1841.171 kb on - strand, within gdhA at 1841.321 kb on + strand, within gdhA at 1841.335 kb on - strand, within gdhA at 1841.335 kb on - strand, within gdhA at 1841.369 kb on - strand, within gdhA at 1841.369 kb on - strand, within gdhA at 1841.374 kb on - strand, within gdhA at 1841.458 kb on + strand, within gdhA at 1841.468 kb on + strand, within gdhA at 1841.468 kb on + strand, within gdhA at 1841.613 kb on - strand at 1841.618 kb on - strand at 1841.635 kb on - strand at 1841.653 kb on - strand at 1841.750 kb on - strand at 1841.750 kb on - strand at 1841.854 kb on + strand at 1841.854 kb on + strand at 1841.875 kb on + strand at 1841.892 kb on + strand at 1841.892 kb on + strand at 1841.900 kb on - strand at 1841.916 kb on + strand at 1841.922 kb on - strand at 1842.093 kb on - strand, within b1762 at 1842.109 kb on + strand, within b1762 at 1842.109 kb on - strand, within b1762 at 1842.174 kb on + strand, within b1762 at 1842.219 kb on + strand, within b1762 at 1842.285 kb on - strand, within b1762 at 1842.285 kb on - strand, within b1762 at 1842.285 kb on - strand, within b1762 at 1842.298 kb on - strand, within b1762 at 1842.331 kb on + strand, within b1762 at 1842.362 kb on - strand, within b1762 at 1842.362 kb on - strand, within b1762 at 1842.376 kb on - strand, within b1762 at 1842.378 kb on + strand, within b1762 at 1842.378 kb on + strand, within b1762 at 1842.380 kb on + strand, within b1762 at 1842.386 kb on - strand, within b1762 at 1842.390 kb on + strand, within b1762 at 1842.499 kb on + strand, within b1762 at 1842.541 kb on + strand, within b1762 at 1842.550 kb on + strand, within b1762 at 1842.574 kb on + strand, within b1762 at 1842.938 kb on - strand at 1842.938 kb on - strand at 1843.030 kb on - strand at 1843.030 kb on - strand at 1843.036 kb on - strand at 1843.180 kb on + strand at 1843.182 kb on + strand at 1843.714 kb on - strand, within topB
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 1,840,876 - gdhA b1761 0.36 -0.2 1,840,886 + gdhA b1761 0.37 -0.9 1,840,886 + gdhA b1761 0.37 -0.9 1,840,971 + gdhA b1761 0.43 -0.4 1,840,971 + gdhA b1761 0.43 +0.4 1,841,012 - gdhA b1761 0.46 -0.0 1,841,111 + gdhA b1761 0.53 +0.3 1,841,171 - gdhA b1761 0.58 -0.2 1,841,321 + gdhA b1761 0.69 -0.1 1,841,335 - gdhA b1761 0.70 -2.3 1,841,335 - gdhA b1761 0.70 -0.9 1,841,369 - gdhA b1761 0.72 -0.3 1,841,369 - gdhA b1761 0.72 +0.5 1,841,374 - gdhA b1761 0.73 +0.5 1,841,458 + gdhA b1761 0.79 -0.1 1,841,468 + gdhA b1761 0.80 +0.4 1,841,468 + gdhA b1761 0.80 -0.0 1,841,613 - -1.1 1,841,618 - +1.2 1,841,635 - +0.1 1,841,653 - +0.2 1,841,750 - +0.9 1,841,750 - +1.7 1,841,854 + -0.2 1,841,854 + -0.3 1,841,875 + +1.1 1,841,892 + +1.2 1,841,892 + +0.3 1,841,900 - +0.1 1,841,916 + +2.3 1,841,922 - +0.3 1,842,093 - b1762 b1762 0.23 +0.3 1,842,109 + b1762 b1762 0.24 -0.1 1,842,109 - b1762 b1762 0.24 -0.2 1,842,174 + b1762 b1762 0.31 +1.3 1,842,219 + b1762 b1762 0.35 -2.3 1,842,285 - b1762 b1762 0.41 -0.3 1,842,285 - b1762 b1762 0.41 +0.7 1,842,285 - b1762 b1762 0.41 -0.8 1,842,298 - b1762 b1762 0.43 +0.7 1,842,331 + b1762 b1762 0.46 +0.7 1,842,362 - b1762 b1762 0.49 +0.4 1,842,362 - b1762 b1762 0.49 -0.3 1,842,376 - b1762 b1762 0.50 -0.3 1,842,378 + b1762 b1762 0.50 -1.0 1,842,378 + b1762 b1762 0.50 +0.7 1,842,380 + b1762 b1762 0.50 +0.7 1,842,386 - b1762 b1762 0.51 +0.7 1,842,390 + b1762 b1762 0.51 +0.7 1,842,499 + b1762 b1762 0.62 +0.3 1,842,541 + b1762 b1762 0.66 +1.5 1,842,550 + b1762 b1762 0.67 -0.3 1,842,574 + b1762 b1762 0.69 +0.7 1,842,938 - +1.7 1,842,938 - +0.2 1,843,030 - -0.2 1,843,030 - -0.1 1,843,036 - -0.1 1,843,180 + +1.0 1,843,182 + -0.7 1,843,714 - topB b1763 0.35 -0.1
Or see this region's nucleotide sequence